Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
40 Poster Abstracts
P037
Treatment of Uterine Infections in Non Cycling Cows with
Cloprostenol
Fernandes, C 1-2 *; Figueiredo, A 1-2 ; Alves, B 2 ; Oliveira, E 2 ; Viana, J 4 , Gioso, M
1; Oba, E 4
1Faculty of Veterinary Medicine, University of Alfenas, Brazil; 2 Biotran LTDA,
Brazil; 3 Embrapa-Gado de Leite, Brazil; 4 FMVZ-Unesp – Botucatu, Brazil
Recent findings on the mechanisms of uterine defense show the
eicosanoids as the main substances that modulate this activity. This
observation open an excellent chance for the treatment of uterine
infections, once the PGF2α and its analogous have activity on the
production of these substances in the uterus. Moreover, they would
provoke reduction in the progesterone (P4). P4 produces substances
that inhibit the uterine defense mechanisms and partially block the
production of eicosanoids. The application of PGF analogous was
capable of stimulating the PGF2 and other related substances
production in the uterus, as Leucotriene B4, which activate some
leucocitary functions, associated to the neutrophile, the most
important cell in the uterus defense mechanism. The aims of this
study were to evaluate and compare the efficiency of protocols of
Cloprostenol administration for the treatment of clinical endometritis
in dairy cows (Holstein) without luteal ovarian activity. Cows of six
herds were used, all presenting clinical endometritis, associated with a
involuted uterus (30-118 days post-partum) and absence of corpus
luteum. The diagnosis and classification of the endometritis was based
on abnormal uterine discharge on vaginoscopic examination. In
accordance with the infection degree (mucopurulent or purulent) the
animals had been randomized in four groups, and received IM
treatments: T1 (n=22): 2ml of saline solution; T2 (n=41): only one
dose of 0.530mg of Cloprostenol (2ml Ciosin Schering Plough-
Brazil); T3 (n=43): 2 doses of 0.530mg of Cloprostenol 24 hours apart
and T4 (n=40): 2 doses 0.530mg of Cloprostenol with interval of 48
hours. The cows were evaluated by vaginoscopy 20 to 30 days later.
The efficiency between the treatments was compared by the χ2 test.
There was no herd effect in treatment results. The efficiency was
18.18a; 39.02b, 71.42c and 47.50%b for groups T1, T2, T3 and T4,
respectively. The treatment with better efficiency was T3, (P5 mm), and number of follicles ≥4 mm on D7 were
analyzed by logistic regression using Glimmix procedure. Daily size
of follicles was analyzed by MIXED procedure. Surprisingly,
treatment with EB did not delay emergence of the follicular wave
(1.40±0.24 vs. 1.40±0.24 days, P=1.00) or alter largest follicle size at
any day post-treatment. In addition, there was no difference between
control and EB-treated cows in growth rate of the largest follicle from
D1 to D7 (1.50±0.05 vs. 1.21±0.16 mm/d; P=0.34) or D4 to D7
(1.20±0.17 vs. 1.40±0.16 mm/d; P=0.53); but, EB decreased growth
of the largest follicle from D1 to D4 (1.25±0.15 vs. 0.83±0.13 mm/d;
P=0.03). Unexpectedly, EB treatment increased number of follicles
≥4mm on Days 5, 6, or 7 (D7; Control=7.2±0.8 vs. EB=21.2±5.3
follicles; P=0.03). Thus, a dose of EB that normally delays follicular
wave emergence by ~4d was not effective in delaying emergence or
growth of the dominant follicle after follicular aspiration perhaps due
to absence of follicular products such as inhibin. However, EB
treatment following follicular aspiration produced a dramatic increase
in the numbers of small follicles even in the presence of a dominant
follicle.
P039
Ovarian Cysts Treatment with Fertirelin Associated or not
to Cloprostenol
Fernandes, C 1-2 ; Figueiredo, A 1-2 *; Alves, B 2 ; Oliveira, E 2 ; Viana, J 4 , Gioso,
M 1 ; Oba, E 4
1Faculty of Veterinary Medicine, University of Alfenas, Brazil; 2 Biotran LTDA,
Brazil; 3 Embrapa-Gado de Leite, Brazil; 4 FMVZ-Unesp – Botucatu, Brazil
The ovarian cysts was a of high occurrence pathology and for causes
significant alterations in the reproductive performance of the animals.
Its treatment must be effective, in the direction to minimize the
reproductive losses. The result of the successful treatment would be
the regression of the cystic structure and formation of a luteal mass
(corpus luteum) and the fast return to the cyclical luteal ovarian
activity and manifestation of regular estrous cycles. Braun et al.
(2000) shows considerable occurrence of cystic structures with
partially luteinized wall in dairy cows, and cite the possibility of
beneficial effect in the application of prostaglandins associated to the
analogous ones of the GnRH for treatment. The objective of this study
was to evaluate and to compare the efficiency of the Fertirelin Acetate
in association or not with the Cloprostenol for treatment of ovarian
cysts in dairy cows. 133 holstein cows between 30 and 90 days postpartum
had been used in four Farms. The cyst diagnosis was made by
ultrasonography (Esaote-Falco), considering as cyst a anecoic
structure above of 20mm beyond the absence of luteal mass in the two
ovaries. The animals had been randomized into two groups that had
received the following treatments, by IM way in only dose: G1
(n=51): 0,1mg of Fertirelin Acetate (Fertigen Schering Plough-
Brazil); G2 (n=49): 0,1mg of Fertirelin Acetate + 0,530mg of
Cloprostenol (Ciosin Schering Plough-Brazil) at the same time,
and G3 (n=35): Control Group (non treated). All the animals again
had been evaluated of 12 the 18 days later. The treatment was
considered efficient where in the second ultrasonography evaluation it
was detected the absence of the cystic structure and presence of luteal
mass. The efficiency of the treatments was evaluated by the χ2 test.
The results of the two treatments had been 54.91; 81.63 and 17.14%,
for the groups G1; G2 and G3, respectively (P