Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 37<br />
Botu-Bov exten<strong>de</strong>rs. Eighteen ejaculates of bulls from Alta Genetics –<br />
Brazil were divi<strong>de</strong>d <strong>in</strong> two fractions and each one was frozen either<br />
with Andromed (M<strong>in</strong>itub) or Botu-Bov (Biotech Botucatu) exten<strong>de</strong>rs,<br />
accord<strong>in</strong>g to the manufacturer recommendation. Andromed: <strong>in</strong>itial<br />
dilution 1:1 (v:v), 10 m<strong>in</strong>utes at 37 o C, dilution aga<strong>in</strong> until f<strong>in</strong>al<br />
concentration, ma<strong>in</strong>tenance for 1 hour at 18 o C, pack<strong>in</strong>g <strong>in</strong> 0.25mL<br />
straws, stabiliz<strong>in</strong>g at 5 o C for 4 hours and freez<strong>in</strong>g <strong>in</strong> the mach<strong>in</strong>e <strong>in</strong> a<br />
curve of -30 o C/m<strong>in</strong>ute. Botu-Bov: total dilution at room temperature,<br />
pack<strong>in</strong>g <strong>in</strong> 0.25mL straws, stabiliz<strong>in</strong>g for 4 hours at 5 o C and freez<strong>in</strong>g<br />
by the same method. Straws were thawed at 37 o C for 30 second <strong>in</strong><br />
water bath, then ma<strong>in</strong>ta<strong>in</strong>ed <strong>in</strong> dry block at 37 o C for 4 hours. Motility<br />
characteristics were evaluated at 10 m<strong>in</strong>utes and 4 hours after thaw<strong>in</strong>g<br />
by CASA (IVOS 12). Integrity of plasma and acrosomal membranes<br />
was evaluated 10 m<strong>in</strong>utes after thaw<strong>in</strong>g by epifluorescence with<br />
Propidium Iodi<strong>de</strong>, FITC-PSA and JC1. At 10 m<strong>in</strong>utes after thaw<strong>in</strong>g,<br />
Botu-Bov exten<strong>de</strong>r was statistically superior (P0.05) between exten<strong>de</strong>rs <strong>in</strong> progressive motility and<br />
acrosomal <strong>in</strong>tegrity at this moment. At 4 hours after thaw<strong>in</strong>g, there<br />
were no differences (P>0.05) <strong>in</strong> total motility (57 vs 64%),<br />
progressive motility (25 vs 30%) and l<strong>in</strong>earity (67 vs 75%) between<br />
Botu-Bov and Andromed, respectively, except for straightness (42 vs<br />
50%, P