Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
Poster Abstracts 37
Botu-Bov extenders. Eighteen ejaculates of bulls from Alta Genetics –
Brazil were divided in two fractions and each one was frozen either
with Andromed (Minitub) or Botu-Bov (Biotech Botucatu) extenders,
according to the manufacturer recommendation. Andromed: initial
dilution 1:1 (v:v), 10 minutes at 37 o C, dilution again until final
concentration, maintenance for 1 hour at 18 o C, packing in 0.25mL
straws, stabilizing at 5 o C for 4 hours and freezing in the machine in a
curve of -30 o C/minute. Botu-Bov: total dilution at room temperature,
packing in 0.25mL straws, stabilizing for 4 hours at 5 o C and freezing
by the same method. Straws were thawed at 37 o C for 30 second in
water bath, then maintained in dry block at 37 o C for 4 hours. Motility
characteristics were evaluated at 10 minutes and 4 hours after thawing
by CASA (IVOS 12). Integrity of plasma and acrosomal membranes
was evaluated 10 minutes after thawing by epifluorescence with
Propidium Iodide, FITC-PSA and JC1. At 10 minutes after thawing,
Botu-Bov extender was statistically superior (P0.05) between extenders in progressive motility and
acrosomal integrity at this moment. At 4 hours after thawing, there
were no differences (P>0.05) in total motility (57 vs 64%),
progressive motility (25 vs 30%) and linearity (67 vs 75%) between
Botu-Bov and Andromed, respectively, except for straightness (42 vs
50%, P