Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 36 Poster Abstracts cows were given a CIDR for 10 d and 500 μg cloprostenol (PGF; Estrumate, Schering-Plough Animal Health) at CIDR removal. Estrus detection (Estrus=D0) was done 3x/d for 5 d after CIDR removal. On D7-8, cows received 2 PGF treatments (12 h apart) and randomly assigned to receive 100 μg GnRH at 36 (Control; n=6), 36 and 38 (n=7) or 36 and 40 h (n=7) after first PGF. Ovulation was determined by ultrasonography. Blood samples (n=15/animal) were collected and plasma analyzed for LH as in Expt 1. Blood samples were also collected every second d from ovulation until 14 d after for plasma P4 concentrations. Data were analyzed using Proc MIXED and Kruskal- Wallis test. In Expt 1, mean (± SE) LH (ng/mL) concentration was affected by treatment (P
16 t h International Congress on Animal Reproduction Poster Abstracts 37 Botu-Bov extenders. Eighteen ejaculates of bulls from Alta Genetics – Brazil were divided in two fractions and each one was frozen either with Andromed (Minitub) or Botu-Bov (Biotech Botucatu) extenders, according to the manufacturer recommendation. Andromed: initial dilution 1:1 (v:v), 10 minutes at 37 o C, dilution again until final concentration, maintenance for 1 hour at 18 o C, packing in 0.25mL straws, stabilizing at 5 o C for 4 hours and freezing in the machine in a curve of -30 o C/minute. Botu-Bov: total dilution at room temperature, packing in 0.25mL straws, stabilizing for 4 hours at 5 o C and freezing by the same method. Straws were thawed at 37 o C for 30 second in water bath, then maintained in dry block at 37 o C for 4 hours. Motility characteristics were evaluated at 10 minutes and 4 hours after thawing by CASA (IVOS 12). Integrity of plasma and acrosomal membranes was evaluated 10 minutes after thawing by epifluorescence with Propidium Iodide, FITC-PSA and JC1. At 10 minutes after thawing, Botu-Bov extender was statistically superior (P0.05) between extenders in progressive motility and acrosomal integrity at this moment. At 4 hours after thawing, there were no differences (P>0.05) in total motility (57 vs 64%), progressive motility (25 vs 30%) and linearity (67 vs 75%) between Botu-Bov and Andromed, respectively, except for straightness (42 vs 50%, P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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