Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 34 Poster Abstracts purpose cows. An important observation was the presence of vagina infection on the sponge treatment group. In conclusion, the use of progesterone devices can improve overall conception rate in double purpose herd. Therefore, the timed AI protocols can be used as tool for reproductive management in tropical farms. P020 The effect of high levels of proximal cytoplasmic droplets on sperm functional competence. Carreira, J 1 *; Koivisto, M 1 ; Mingoti, G 1 ; Leite, J 1 ; Perri, S 1 ; Rodrigues, L 2 1São Paulo State University, Araçatuba, Brazil; 2 Lagoa da Serra Artificial Insemination Centre, Sertãozinho, Brazil Introduction Semen conventional analysis may not adequately represent the diverse number of biological properties that this highspecialized cell can express (Hum. Repr.18:1023-1028,2003). The use of more objective techniques, such as fluorescent probes to assess the integrity of membranes and mitochondrial function of the sperm may provide data on the occurrence of cryoinjuries (Acta Sci.Vet.33:327- 327, 2005). Frozen-thawed ejaculates with high levels of proximal cytoplasmic droplets (PCD) produces low fertility rates (J.Repr.Fert.51:109-116, 1997). The goal of this study was to evaluate plasma membrane integrity (PL), acrossome status (ACR) and mitocondrial function (MIT) of bovine semen with high levels of PCD after cryopreservation, comparing to samples with normal parameters. Methods Three batches of five (control group G1: with ≤ 15% of total defects) and eight Bos indicus bulls (G2: ≥15% PCD) were analyzed. The following tests were carried out: post thaw motility (MOT), vigor (VI), concentration (CO) and morphology. The samples were centrifuged (750rpm/ 5 min) twice with 500μL of TALP-Semen. CO was adjusted to 25x10 6 sptz/mL. To an 30μL aliquot, 2μL of PI (0,2mg/mL), 1,6 μL of JC-1 (0,5mg/mL) and 10 μL FITC-PSA (100μg/mL) was added, after incubation at 38.5°C/8 min, 8μL was evaluated by epifluorescent microscopy. Data were analyzed with ANOVA. Results MOT, VI and CO were nor significant in both groups (MOT: 45.42 vs 40.31; VI: 4.47 vs 4.03; CO: 29.18 vs 31.23 x10 6 sptz/mL for G1 and G2, respectively). The percentage of PCD (G1= 0.51%; G2=24.35%), major defects (4.98% G1; 38.9% G2), and total defects (G1 10.32%; G2 48.38%) were significantly higher in G1 when compared to G2. Minor defects were not different between groups (G1= 5.38%; G2= 9.48%). G1 had significant higher results for ACR (38.48% vs 29.70% G2), PL (38.16% vs 21.39%) and also MIT (41.90% vs 23.22%) for G1 and G2, respectively. Conclusion The results indicate that semen with high levels of PCD may be functionally deficient and more susceptible to cryoinjuries as show the lower levels of acrossome, membrane integrity and mitochondrial potential. P021 Evaluation of slow-release parenteral natural progesterone and its effects in a modified Ovsynch protocol in Holstein dairy heifers Cavestany, D 1,2 *; Sanchez, A 3 ; Fernandez, D 3 ; Salazar, E 3 ; Leyton, L 4 Crespi, D 2 , Meikle, A 5 1National Institute of Agricultural Research (INIA) Uruguay; 2 Department of Reproduction, Veterinary Faculty, Uruguay; 3 Private veterinarians, Uruguay; 4Faculty of Agronomy, University of El Salvador, San Salvador; 5 Laboratory of Nuclear Techniques, Faculty of Veterinary, Uruguay The efficiency of the ovulation synchronisation (Ovsynch/TAI protocol) can be improved by progesterone (P4) supplementation and this increases the ovulation synchrony and pregnancy rate. The most common P4 sources are intra-vaginal devices impregnated by natural P4 and/or oral progestagens. In this experiment a slow-release parenteral natural P4 was tested in the above-mentioned protocol. Three trials were conducted. In trial 1, three multiparous milking Holstein-Friesian (HF) cows (511±28 kg) and 3 HF heifers (372±21 kg) received three prostaglandin (PG) treatments at weekly intervals, after which they received 400 mg of progesterone (4-pregnano-3.20- dione) (Laboratory Rio de Janeiro, Santa Fé, Argentina) subcutaneously and were bled frequently for 5 days to check plasma P4 levels. Peak P4 levels were achieved 4 hours post treatment (10.2±0.8 & 6.2±0.7 ng/mL, P0.1) for cows and heifers. In trial 2, one-hundred-eleven heifers (374±4 kg) were allocated into 2 groups (control: Ovsych and treatment: Ovsynch + 400 mg P4 SC at the time of the GnRH treatment). Oestrus detection was done from day 5, and heifers in heat were inseminated. While synchrony of ovulations improved in the treated group, 78% vs. 52% (P
16 t h International Congress on Animal Reproduction Poster Abstracts 35 0.06) for being higher than those of treated recipients without aCL (6.8 ± 0.8 ng/ml, respectively). In conclusion, luteal hCG treatment enhanced the survival of lowviability embryos (half-embryos) in low endogenous P4 concentrations recipients (high-yielding lactating dairy cows). This effect was associated to the increased plasma P4 concentrations in recipients with induced aCL. Acknowledgements – This experiment was funded by the grant CIISA 48 Embrio-ovárica. P023 A successful estrus synchronization method (Doublesynch) for timed artificial insemination in anestrous dairy cows Öztürk, ÖA; Cirit, Ü*; Baran, A; Ak, K Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey Recently, Cirit et al. (2007) have developed a new synchronization method (Doublesynch) by injecting an additional PGF 2α two d before the Ovsynch protocol. Doublesynch program increased pregnancy rates 22.2 percentage units (50.0% vs. 72.2%), however, statistical significance (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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