Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 30 Poster Abstracts suckling restriction (TSR) practice with bypass fat supplementation (BFS) on the duration of the open days, and on pregnancy at day 100 days in a commercial beef cattle production in Colombia. Materials and methods 32 commercial zebu cows were used and three groups were designed: group I control (n=10) cow and calf in permanent contact, group II TSR (n=10) cows with a mean of 65.7 days postpartum and TSR during 72 hours with heat detection, group III TSR + BFS (n=12), cows supplemented from the 32 postpartum day with BFS and TSR of 72 hours in 62 postpartum day with heat detection. A chi-square test was realized to establish association between the different groups and pregnancy at day 100 of the experiment and a Kruskall-Wallis test was realized to establish differences between the groups in the open days number. These analyses were performed using SAS ver 8.0. Results Although there was no association between the different groups and pregnancy at day 100 of the experiment (X 2 = 4.097, P- value = 0.1289), we found a tendency in the proportion of pregnant cows in group II TSR and group III TSR + BFS. Besides, we found statistical significative differences between the groups in the open days number (Kruskall-Wallis test, test value = 8,70259, p-value = 0,0128901). In the group III TSR + BFS we found less open days (83.3) followed by group II TSR (97.1) and group I control (136.3) respectively. Conclusions We recommend the use of bypass fat supplementation with temporal suckling restriction as an alternative to decrease the open days period in Colombian beef cattle production. P008 Color-Doppler ultrasonography: a non-invasive method to assess luteolysis in cattle Araujo, RR 1,2 *, Ginther, OJ 1 , Ferreira, JC 3 , Siqueira, LGB 4 , Beg, MA 1 , Wiltbank, MC 5 1Pathobiological Sciences, University of Wisconsin - Madison, United States; 2Dairy Science, University of Wisconsin - Madison, United States; 3 Eutheria Foundation - Cross Plains - Wi, United States; 4 Wcvm - Lacs, University of Saskatchewan, United States; 5 Dairy Science, University of Wisconsin - Madison, United States Many studies have shown the relationship of corpus luteum (CL) echotexture with functional status using B-mode ultrasonography. Recently, Doppler ultrasonography was introduced as an additional method for evaluating the structural and functional attributes of the CL. Two ultrasound technologies (B-mode and Color-Doppler) were used for comparing CL morphology with systemic progesterone concentrations. Holstein heifers (n=14) were scanned using a duplex B-mode and pulsed-wave color-Doppler ultrasound instrument (Aloka SSD 3500; Aloka America, Wallingford, CT) equipped with a lineararray 7.5 MHz transducer. The scanning was performed daily starting 9 days after ovulation until the next ovulation. Real-time B- mode/color-Doppler images of the continuous scans were recorded with an online digital videotaping system. During each scanning, percentage of CL area with color-Doppler signals for blood flow was estimated. Blood samples were collected once a day for plasma progesterone assay. Computer-assisted analyses of B-mode images for mean pixel value, based on gray scale (0=black to 255=white), were performed using a custom-developed software. Luteal blood flow, mean pixel value and plasma progesterone were analyzed by SAS MIXED procedure with a REPEATED measures statement and differences among days were determined by paired t-tests. Pearson’s correlation coefficients were calculated between luteal blood flow, mean pixel value, and plasma progesterone. Data were normalized to the examination in each heifer when the decreasing plasma progesterone concentrations reached
16 t h International Congress on Animal Reproduction Poster Abstracts 31 PGF2α[PG]-56h-GnRH[G2]-18h-AI). Ovsynch+FSH cows received 20 mg of FSH at time of PG. Ovarian ultrasound was performed at G1, PG, G2 and 6d after G2. Blood samples were collected at G1, PG, G2 and 13d after G2 to determine circulating P4 and/or E2. Luteolysis was defined as P4
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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