Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 29<br />
hyperimmune serum were produced aga<strong>in</strong>st Arcanobacterium<br />
pyogenes and E. coli that were isolated <strong>in</strong> Iranian dairy farms. Cows<br />
<strong>in</strong> group oxytetracycl<strong>in</strong>e (n=39) were treated with one <strong>in</strong>trauter<strong>in</strong>e<br />
<strong>in</strong>fusion of 5gr oxytetracycl<strong>in</strong>e (10%). Cows that did not show any<br />
cl<strong>in</strong>ical signs of postpartum endometritis were regar<strong>de</strong>d as healthy<br />
control group (HC, n =91). In group hyperimmunue serum and<br />
oxytetracycl<strong>in</strong>e (OTC), all cows were re-exam<strong>in</strong>ed 39–49 DIM. In<br />
group hyperimmunue serum and OTC, cows were re-treated with<br />
hyperimmunue serum if signs of endometritis were found. Cure rate<br />
after the first treatment, <strong>de</strong>f<strong>in</strong>ed as the absence of vag<strong>in</strong>al discharge at<br />
the re-exam<strong>in</strong>ations, was 64.3 % and 61.5% <strong>in</strong> groups hyperimmunue<br />
Serum and OTC (P > 0.05). Reproductive performance measures<br />
showed no significant differences (P > 0.05) between the two<br />
treatment groups. There was no significant difference (P > 0.05)<br />
Service rate between treatments groups hyperimmunue Serum and<br />
OTC, compared to HC. Conception rates to all services and<br />
percentages of cows pregnant by 180 DIM were 90.48, 86.49 and<br />
92.13 <strong>in</strong> groups hyperimmunue Serum, OTC and HC, respectively (P<br />
> 0.05). In both treatment groups, cure rate and reproductive<br />
performance measures were better for cows categorized E1 or E2,<br />
than for cows categorized E3 but the differences were not significant.<br />
Conception rate to all services for cows with endometritis (category<br />
E1, E2 and E3) was 52.94 <strong>in</strong> group hyperimmunue Serum and 57.14<br />
<strong>in</strong> group OTC compared 66.67 <strong>in</strong> HC (P > 0.05). The results of this<br />
field trial suggest that hyperimmunue serum could be the alternative<br />
no antibiotic treatment of choice for postpartum endometritis <strong>in</strong> dairy<br />
cattle.<br />
P005<br />
GnRH-<strong>in</strong>duced LH and ovulatory responses <strong>in</strong> dairy<br />
heifers dur<strong>in</strong>g diestrus and proestrus<br />
Ambrose, DJ 1 *, Colazo, MG 1 , Bhuwanee, A 2 , Kumpula, B 2 , Lamont, AL 2 ,<br />
Salmon, F 2 , Suddaby, P 2 , Terletski, S 2<br />
1Agriculture Research Division, Alberta Agriculture and Food, Canada;<br />
2Agricultural Food and Nutritional Science, University of Alberta, Canada<br />
The objective was to <strong>de</strong>term<strong>in</strong>e whether pituitary LH response to<br />
GnRH <strong>in</strong> Holste<strong>in</strong> heifers differed between diestrous and proestrous<br />
stages of the estrous cycle. Fifteen postpuberal heifers (13 to 14 mo of<br />
age, 404 to 479 kg body weight) were given 25 mg of d<strong>in</strong>oprost<br />
trometham<strong>in</strong>e (PGF; Lutalyse, Pfizer Animal Health) im. Estrus<br />
<strong>de</strong>tection was done twice daily for 4 d after PGF treatment and<br />
ovulation (Day 0) confirmed by ultrasonography. On Day (Mean ±<br />
SE) 8.5 ± 0.6 after ovulation, 100 µg of gonadorel<strong>in</strong> acetate (GnRH;<br />
Fertil<strong>in</strong>e, Vetoqu<strong>in</strong>ol Canada Inc.) was given im to all heifers<br />
(Diestrous stage treatment). Blood samples (n=15/heifer; from 15 m<strong>in</strong><br />
before to 8 h after GnRH) were collected and plasma analyzed for LH<br />
concentration. One blood sample taken prior to GnRH was analyzed<br />
for progesterone. Ovulation was <strong>de</strong>term<strong>in</strong>ed by ultrasonography 48 h<br />
follow<strong>in</strong>g GnRH treatment and heifers received an <strong>in</strong>travag<strong>in</strong>al<br />
progesterone (P4; 1.9 g) <strong>in</strong>sert (CIDR; Pfizer Animal Health) for 7 d,<br />
with PGF adm<strong>in</strong>istered at CIDR removal. Twenty-four h after CIDR<br />
removal (Day 15.9 ± 0.6), GnRH was given im (Proestrous stage<br />
treatment). Ovulatory response, P4 and LH concentrations were<br />
<strong>de</strong>term<strong>in</strong>ed as <strong>in</strong> Diestrus. Data were analyzed by Proc MIXED,<br />
ANOVA, and Chi-square. Two heifers did not respond to PGF<br />
treatment; both were exclu<strong>de</strong>d from the study. Plasma P4<br />
concentration (ng/mL) at GnRH treatment was higher (P < 0.01) <strong>in</strong><br />
heifers treated dur<strong>in</strong>g diestrus (5.7 ± 0.7) than dur<strong>in</strong>g proestrus (1.0 ±<br />
0.2). The ovulatory response to GnRH treatment was higher dur<strong>in</strong>g<br />
proestrus than diestrus (P < 0.02; 61.5 vs 15.4%). GnRH-<strong>in</strong>duced LH<br />
peak was lower (P < 0.04; 5.7 ± 0.8 vs 13.6 ± 3.0 ng/mL) and of<br />
shorter duration (P < 0.01; 5.4 ± 0.3 vs 6.8 ± 0.3 h) <strong>in</strong> heifers treated<br />
dur<strong>in</strong>g diestrus than dur<strong>in</strong>g proestrus. Regardless of the stage of the<br />
estrous cycle at treatment, heifers that ovulated after GnRH had a<br />
significantly longer <strong>in</strong>terval from GnRH treatment to LH peak than<br />
those that did not ovulate (P < 0.01; 1.7 ± 0.1 vs 1.0 ± 0.1 h).<br />
However, <strong>in</strong> heifers treated with GnRH dur<strong>in</strong>g proestrus, LH<br />
concentrations did not differ (P = 0.60) between those that ovulated<br />
and those that did not. In conclusion, heifers treated with GnRH<br />
dur<strong>in</strong>g diestrus had lower peak LH concentrations, <strong>de</strong>layed atta<strong>in</strong>ment<br />
of LH peak, shorter duration of elevated LH concentrations, and lower<br />
ovulatory responses than those treated dur<strong>in</strong>g proestrus.<br />
P006<br />
The Effect of Add<strong>in</strong>g Cholesterol, Vitam<strong>in</strong> A, Cod Liver or<br />
Flax Oil Loa<strong>de</strong>d Cyclo<strong>de</strong>xtr<strong>in</strong> on Bull Sperm Cryosurvival<br />
Amorim, EAM 1,2 *, Graham, J 2 , Spizziri, B 2 , Meyers, M 2 , Amorim, LS 1,2 , Torres,<br />
CAA 1<br />
1Department of Animal Science, Fe<strong>de</strong>ral University of Vicosa, Brazil;<br />
2Department of Biomedical Sciences, Colorado State University, USA<br />
Introduction Damage occurr<strong>in</strong>g to spermatozoa dur<strong>in</strong>g<br />
cryopreservation results <strong>in</strong> a loss of motile cells and cells that are<br />
functionally normal, compared to fresh sperm samples. Treat<strong>in</strong>g bull<br />
sperm with cholesterol-loa<strong>de</strong>d cyclo<strong>de</strong>xtr<strong>in</strong>s (CLCs) prior to<br />
cryopreservation results <strong>in</strong> <strong>in</strong>creased sperm cryosurvival, however,<br />
add<strong>in</strong>g cholesterol to bull sperm membranes alters their ability to<br />
capacitate <strong>in</strong> vitro. This study compared the effect of add<strong>in</strong>g four<br />
compounds, which should <strong>in</strong>corporate <strong>in</strong>to and <strong>in</strong>crease membrane<br />
fluidity at low temperatures thereby <strong>in</strong>creas<strong>in</strong>g cryosurvival.<br />
Method Twenty-five ejaculates from four bulls were collected us<strong>in</strong>g<br />
artificial vag<strong>in</strong>a and exten<strong>de</strong>d with Tris, and then sub-divi<strong>de</strong>d <strong>in</strong>to 11<br />
treatments: No additive (negative control); 1.5 mg CLC/120 million<br />
sperm (positive control); and 0.75 mg, 1.5 mg and 3.0 mg/120 million<br />
sperm of cyclo<strong>de</strong>xtr<strong>in</strong> pre-loa<strong>de</strong>d with vitam<strong>in</strong> A or Cod Liver oil or<br />
Flax oil. Methyl-β-cyclo<strong>de</strong>xtr<strong>in</strong> was preloa<strong>de</strong>d with cholesterol as<br />
<strong>de</strong>scribed by Purdy and Graham (2004). Spermatozoa were <strong>in</strong>cubated<br />
for 15 m<strong>in</strong> at 22 o C. Then, samples were diluted 1:1 (v:v) <strong>in</strong> Tris with<br />
20% Egg Yolk (EY) and cooled to 5 o C. After dilution 1:1 (v:v) with<br />
Tris conta<strong>in</strong><strong>in</strong>g 10% EY and 16% glycerol, samples were allowed to<br />
equilibrate for 15 m<strong>in</strong> before packag<strong>in</strong>g <strong>in</strong>to 0.5 ml straws, freez<strong>in</strong>g <strong>in</strong><br />
static liquid nitrogen vapor (4.5 cm above the liquid nitrogen) for 20<br />
m<strong>in</strong> and plung<strong>in</strong>g <strong>in</strong>to liquid nitrogen for storage. Straws were thawed<br />
<strong>in</strong> a water bath at 37ºC for 30 sec and the percentages of total motile<br />
<strong>in</strong> each sample were <strong>de</strong>term<strong>in</strong>ed us<strong>in</strong>g a computer-assisted sperm<br />
analysis (Hamilton Thorne Motility Analyser).<br />
Results The percentages of motile total sperm cells were ma<strong>in</strong>ta<strong>in</strong>ed<br />
after thaw<strong>in</strong>g for bull sperm treated with CLC (45%) compared to all<br />
other treatments (26-41%; P0.05).<br />
Conclusion Therefore, <strong>in</strong>creas<strong>in</strong>g membrane cholesterol levels by<br />
add<strong>in</strong>g CLCs to bull sperm, prior to freez<strong>in</strong>g, is a simple technology<br />
that improved cell cryosurvival, whereas treatments with<br />
cyclo<strong>de</strong>xtr<strong>in</strong>s preloa<strong>de</strong>d with other molecules did not.<br />
Acknowledgments Supported by Colorado State University<br />
Experiment Station and CNPq/Brazil.<br />
P007<br />
Bypass fat supplementation <strong>in</strong> zebu cows (Bos <strong>in</strong>dicus)<br />
<strong>in</strong> the early postpartum: an alternative to <strong>de</strong>crease the<br />
open days period<br />
Mesa, C 1 ; Mahecha, L 2 ; Ruiz, ZT 1 ; Gallo, J 3 ; Angulo, J 1,2 *; Olivera-Angel, M 1<br />
1Grupo <strong>de</strong> Reproducción - Fisiología y Biotecnología, <strong>Facultad</strong> <strong>de</strong> <strong>Ciencias</strong><br />
Agrarias, Universidad <strong>de</strong> Antioquia, Colombia; 2 Grupo Grica, <strong>Facultad</strong> <strong>de</strong><br />
<strong>Ciencias</strong> Agrarias, Universidad <strong>de</strong> Antioquia, Colombia; 3 Soluciones<br />
Nutricionales S.A., Me<strong>de</strong>llín, Colombia<br />
Introduction Traditional beef cattle production <strong>in</strong> tropical South<br />
America is extensive and based primarily on Bos <strong>in</strong>dicus cattle and its<br />
crosses. This productive system <strong>in</strong>volves the permanent contact of the<br />
cow with its calf, feed<strong>in</strong>g with low nutritional value grass and the<br />
highly seasonal supply of forage. These characteristics are the ma<strong>in</strong><br />
causes of postpartum anoestrus and the long calv<strong>in</strong>g to conception<br />
period (open days). Nowadays, there are some management strategies<br />
which help to <strong>de</strong>crease the postpartum anoestrus and the open days.<br />
The use of bypass fat <strong>in</strong> or<strong>de</strong>r to <strong>in</strong>crease the energetic contribution of<br />
the diet, and the restricted suckl<strong>in</strong>g practices, are some of them.<br />
However, these practices are usually used <strong>in</strong> a separated way. The<br />
objective of this study was to evaluate the effect of mix<strong>in</strong>g a temporal