Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 28 Poster Abstracts POSTER ABSTRACTS Poster 01 - Bovine Reproduction P001 New Trends for Estrus Synchronization in Lactating Dairy Cows Amer, HA 1 , Abaza, F 2 * 1Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University; 2 Department of Military Veterinary Services, Ministry of Defense, Egypt This study was conducted to through the light on estrus synchronization in dairy cows. Four treatments were performed on sixty Holstein Friesian cows. In treatment 1 (PP), cows received 2 injections of PGF2α on days 0 and 11 (n=14). In treatment 2 (PGP), cows injected twice PGF2α on days 0 and 11 and 100 ug of GnRH on day 3 after 1st injection (n=14). In treatment 3 (PGPE-0), cows treated with PGP and 1 mg of estradiol cypionate (ECP) at same time of 2nd PGF2α dose (n=16). In treatment 4 (PGPE-1), cows were treated with PGP and 1 mg ECP one day after 2nd PGF2α injection (n=16). Cows were examined rectally and ultrasonographically by B-mode System [Pie-Medical Scanner-240] with a 6-8 MHz linear probe. Every cow was blood sampled at selected intervals for progesterone and estradiol assay and inseminated at estrus. The results showed a higher percentage of GnRH-treated cows (PGP) ovulated after 1 st PGF2α injection than non treated (PP) cows (64.3% vs 50%; P
16 t h International Congress on Animal Reproduction Poster Abstracts 29 hyperimmune serum were produced against Arcanobacterium pyogenes and E. coli that were isolated in Iranian dairy farms. Cows in group oxytetracycline (n=39) were treated with one intrauterine infusion of 5gr oxytetracycline (10%). Cows that did not show any clinical signs of postpartum endometritis were regarded as healthy control group (HC, n =91). In group hyperimmunue serum and oxytetracycline (OTC), all cows were re-examined 39–49 DIM. In group hyperimmunue serum and OTC, cows were re-treated with hyperimmunue serum if signs of endometritis were found. Cure rate after the first treatment, defined as the absence of vaginal discharge at the re-examinations, was 64.3 % and 61.5% in groups hyperimmunue Serum and OTC (P > 0.05). Reproductive performance measures showed no significant differences (P > 0.05) between the two treatment groups. There was no significant difference (P > 0.05) Service rate between treatments groups hyperimmunue Serum and OTC, compared to HC. Conception rates to all services and percentages of cows pregnant by 180 DIM were 90.48, 86.49 and 92.13 in groups hyperimmunue Serum, OTC and HC, respectively (P > 0.05). In both treatment groups, cure rate and reproductive performance measures were better for cows categorized E1 or E2, than for cows categorized E3 but the differences were not significant. Conception rate to all services for cows with endometritis (category E1, E2 and E3) was 52.94 in group hyperimmunue Serum and 57.14 in group OTC compared 66.67 in HC (P > 0.05). The results of this field trial suggest that hyperimmunue serum could be the alternative no antibiotic treatment of choice for postpartum endometritis in dairy cattle. P005 GnRH-induced LH and ovulatory responses in dairy heifers during diestrus and proestrus Ambrose, DJ 1 *, Colazo, MG 1 , Bhuwanee, A 2 , Kumpula, B 2 , Lamont, AL 2 , Salmon, F 2 , Suddaby, P 2 , Terletski, S 2 1Agriculture Research Division, Alberta Agriculture and Food, Canada; 2Agricultural Food and Nutritional Science, University of Alberta, Canada The objective was to determine whether pituitary LH response to GnRH in Holstein heifers differed between diestrous and proestrous stages of the estrous cycle. Fifteen postpuberal heifers (13 to 14 mo of age, 404 to 479 kg body weight) were given 25 mg of dinoprost tromethamine (PGF; Lutalyse, Pfizer Animal Health) im. Estrus detection was done twice daily for 4 d after PGF treatment and ovulation (Day 0) confirmed by ultrasonography. On Day (Mean ± SE) 8.5 ± 0.6 after ovulation, 100 µg of gonadorelin acetate (GnRH; Fertiline, Vetoquinol Canada Inc.) was given im to all heifers (Diestrous stage treatment). Blood samples (n=15/heifer; from 15 min before to 8 h after GnRH) were collected and plasma analyzed for LH concentration. One blood sample taken prior to GnRH was analyzed for progesterone. Ovulation was determined by ultrasonography 48 h following GnRH treatment and heifers received an intravaginal progesterone (P4; 1.9 g) insert (CIDR; Pfizer Animal Health) for 7 d, with PGF administered at CIDR removal. Twenty-four h after CIDR removal (Day 15.9 ± 0.6), GnRH was given im (Proestrous stage treatment). Ovulatory response, P4 and LH concentrations were determined as in Diestrus. Data were analyzed by Proc MIXED, ANOVA, and Chi-square. Two heifers did not respond to PGF treatment; both were excluded from the study. Plasma P4 concentration (ng/mL) at GnRH treatment was higher (P < 0.01) in heifers treated during diestrus (5.7 ± 0.7) than during proestrus (1.0 ± 0.2). The ovulatory response to GnRH treatment was higher during proestrus than diestrus (P < 0.02; 61.5 vs 15.4%). GnRH-induced LH peak was lower (P < 0.04; 5.7 ± 0.8 vs 13.6 ± 3.0 ng/mL) and of shorter duration (P < 0.01; 5.4 ± 0.3 vs 6.8 ± 0.3 h) in heifers treated during diestrus than during proestrus. Regardless of the stage of the estrous cycle at treatment, heifers that ovulated after GnRH had a significantly longer interval from GnRH treatment to LH peak than those that did not ovulate (P < 0.01; 1.7 ± 0.1 vs 1.0 ± 0.1 h). However, in heifers treated with GnRH during proestrus, LH concentrations did not differ (P = 0.60) between those that ovulated and those that did not. In conclusion, heifers treated with GnRH during diestrus had lower peak LH concentrations, delayed attainment of LH peak, shorter duration of elevated LH concentrations, and lower ovulatory responses than those treated during proestrus. P006 The Effect of Adding Cholesterol, Vitamin A, Cod Liver or Flax Oil Loaded Cyclodextrin on Bull Sperm Cryosurvival Amorim, EAM 1,2 *, Graham, J 2 , Spizziri, B 2 , Meyers, M 2 , Amorim, LS 1,2 , Torres, CAA 1 1Department of Animal Science, Federal University of Vicosa, Brazil; 2Department of Biomedical Sciences, Colorado State University, USA Introduction Damage occurring to spermatozoa during cryopreservation results in a loss of motile cells and cells that are functionally normal, compared to fresh sperm samples. Treating bull sperm with cholesterol-loaded cyclodextrins (CLCs) prior to cryopreservation results in increased sperm cryosurvival, however, adding cholesterol to bull sperm membranes alters their ability to capacitate in vitro. This study compared the effect of adding four compounds, which should incorporate into and increase membrane fluidity at low temperatures thereby increasing cryosurvival. Method Twenty-five ejaculates from four bulls were collected using artificial vagina and extended with Tris, and then sub-divided into 11 treatments: No additive (negative control); 1.5 mg CLC/120 million sperm (positive control); and 0.75 mg, 1.5 mg and 3.0 mg/120 million sperm of cyclodextrin pre-loaded with vitamin A or Cod Liver oil or Flax oil. Methyl-β-cyclodextrin was preloaded with cholesterol as described by Purdy and Graham (2004). Spermatozoa were incubated for 15 min at 22 o C. Then, samples were diluted 1:1 (v:v) in Tris with 20% Egg Yolk (EY) and cooled to 5 o C. After dilution 1:1 (v:v) with Tris containing 10% EY and 16% glycerol, samples were allowed to equilibrate for 15 min before packaging into 0.5 ml straws, freezing in static liquid nitrogen vapor (4.5 cm above the liquid nitrogen) for 20 min and plunging into liquid nitrogen for storage. Straws were thawed in a water bath at 37ºC for 30 sec and the percentages of total motile in each sample were determined using a computer-assisted sperm analysis (Hamilton Thorne Motility Analyser). Results The percentages of motile total sperm cells were maintained after thawing for bull sperm treated with CLC (45%) compared to all other treatments (26-41%; P0.05). Conclusion Therefore, increasing membrane cholesterol levels by adding CLCs to bull sperm, prior to freezing, is a simple technology that improved cell cryosurvival, whereas treatments with cyclodextrins preloaded with other molecules did not. Acknowledgments Supported by Colorado State University Experiment Station and CNPq/Brazil. P007 Bypass fat supplementation in zebu cows (Bos indicus) in the early postpartum: an alternative to decrease the open days period Mesa, C 1 ; Mahecha, L 2 ; Ruiz, ZT 1 ; Gallo, J 3 ; Angulo, J 1,2 *; Olivera-Angel, M 1 1Grupo de Reproducción - Fisiología y Biotecnología, Facultad de Ciencias Agrarias, Universidad de Antioquia, Colombia; 2 Grupo Grica, Facultad de Ciencias Agrarias, Universidad de Antioquia, Colombia; 3 Soluciones Nutricionales S.A., Medellín, Colombia Introduction Traditional beef cattle production in tropical South America is extensive and based primarily on Bos indicus cattle and its crosses. This productive system involves the permanent contact of the cow with its calf, feeding with low nutritional value grass and the highly seasonal supply of forage. These characteristics are the main causes of postpartum anoestrus and the long calving to conception period (open days). Nowadays, there are some management strategies which help to decrease the postpartum anoestrus and the open days. The use of bypass fat in order to increase the energetic contribution of the diet, and the restricted suckling practices, are some of them. However, these practices are usually used in a separated way. The objective of this study was to evaluate the effect of mixing a temporal
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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