Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 219<br />
<strong>in</strong>ci<strong>de</strong>nce of subcl<strong>in</strong>ical hypocalcaemia varies between 5% and 10%<br />
of all dairy cows, it could be a safe of money.<br />
P579<br />
Boar sperm quality after addition of pentoxifyll<strong>in</strong>e to<br />
short-term exten<strong>de</strong>r<br />
Yeste, M*; Briz, M; P<strong>in</strong>art, E; Sancho, S; Bussalleu, E; Casas, I; Fàbrega, A;<br />
Puigmulé, M; Garcia-Bonavila, E; Bonet, S<br />
Biotechnology of Animal and Human <strong>Reproduction</strong>, Department of Biology,<br />
University of Girona<br />
Pentoxifyll<strong>in</strong>e has been used <strong>in</strong> humans to improve sperm motility <strong>in</strong><br />
low motility ejaculates and for <strong>in</strong>duc<strong>in</strong>g an early onset of sperm<br />
capacitation. The aim of the present study was to assess sperm<br />
viability, motility, morphology and capacitation after add<strong>in</strong>g five<br />
different concentrations of pentoxifyll<strong>in</strong>e (Ptx): 0.25, 0.5, 1, 2 and 4<br />
mM to semen diluted <strong>in</strong> Beltsville thaw<strong>in</strong>g solution (BTS) over twoday<br />
of storage at 15ºC. Semen samples were obta<strong>in</strong>ed from 21 healthy<br />
Piétra<strong>in</strong> post-pubertal boars and sperm parameters were <strong>de</strong>term<strong>in</strong>ed<br />
before (control) and after apply<strong>in</strong>g the five treatments. Sperm viability<br />
was assessed us<strong>in</strong>g a multiple fluorochrome sta<strong>in</strong><strong>in</strong>g procedure, sperm<br />
motility and morphology by means of computer assisted sperm<br />
analysis (CASA) and the capacitation status us<strong>in</strong>g chlortetracycl<strong>in</strong>e<br />
(CTC) antibiotic sta<strong>in</strong><strong>in</strong>g. In each case, the various sperm parameters<br />
outl<strong>in</strong>ed above were assessed after <strong>in</strong>cubation at 37ºC for 15 m<strong>in</strong> (day<br />
0), or after 1 or 2 days of stor<strong>in</strong>g at 15 ºC. Data were transformed to<br />
arcs<strong>in</strong> √x when necessary for accomplish<strong>in</strong>g normality assumptions<br />
and then analysed with a repeated measures ANOVA and post-hoc<br />
Dunnet’s test. The level of significance was set at P