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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal <strong>Reproduction</strong><br />

218 Poster Abstracts<br />

southwest of Ch<strong>in</strong>a. Gayal has a calv<strong>in</strong>g behavior that the cows are<br />

out of the herd lonely when calv<strong>in</strong>g. The special calv<strong>in</strong>g behavior has<br />

not been covered so far. The objective of this study was, through the<br />

<strong>in</strong>vestigation of calv<strong>in</strong>g behavior of to collect relevant <strong>in</strong>formation on<br />

ethology, which, hopefully, would benefit <strong>in</strong> protect<strong>in</strong>g the herds <strong>in</strong><br />

immigration and feed<strong>in</strong>g management whenever mak<strong>in</strong>g the most of<br />

gayal scientifically. The study was carried out on the Mithun<br />

conservation farm of Phenix mounta<strong>in</strong> at Lushui county <strong>in</strong> Yunnan<br />

prov<strong>in</strong>ce (98°59′--99°03′E, 25°58′--26°04′N, an altitu<strong>de</strong> of 2700--<br />

3200m)from June 1 st to 21 st , 2007, by observ<strong>in</strong>g three gayal cows<br />

be<strong>in</strong>g about to <strong>de</strong>livery(two of multiparous, one of primiparous,<br />

average age of 9.0±5.6) and one adult bull(age of 3.5 years old) un<strong>de</strong>r<br />

free graz<strong>in</strong>g on two hectare fenced improved pasture consist<strong>in</strong>g of<br />

Dactylis glomerata, Trifolium repense and free graz<strong>in</strong>g on the sward<br />

of native grasses with the same management. Average temperature<br />

and relative humidity recor<strong>de</strong>d dur<strong>in</strong>g the experiment were<br />

16.85±1.01 and 74.27±4.54%, respectively to an elevation of<br />

2420m. Relative behavior was recor<strong>de</strong>d with digital vidicon and<br />

camera on Day 3 before the parturition, and monitored gayal cow with<br />

full track at daylight and at every 2h <strong>in</strong>terval for 30 m<strong>in</strong> at night. The<br />

results were shown that gayal behaved out a series of preparative<br />

behavior before the <strong>de</strong>livery, namely calv<strong>in</strong>g behavior were expressed<br />

as: 60% cows would leave the herd to look for food and proper<br />

<strong>de</strong>liver<strong>in</strong>g place alone from 10 to 3 days before calv<strong>in</strong>g, prevent<strong>in</strong>g<br />

milk suck<strong>in</strong>g from the previous calves with more watchful walk<strong>in</strong>g<br />

from 24 to 12hours before calv<strong>in</strong>g. Impatience and mount<strong>in</strong>g behavior<br />

(<strong>in</strong>clud<strong>in</strong>g mount<strong>in</strong>g on gayal bull) of the cows were observed from 8<br />

to 1 hour before calv<strong>in</strong>g. In a few hours before calv<strong>in</strong>g, Gayal cows<br />

performed strongly the behavior of signory, ru<strong>de</strong>ly chas<strong>in</strong>g the other<br />

animals. Rather flat sward on the higher place with lower gradient<br />

was naturally selected by cows as parturition site, where the tall grass<br />

<strong>in</strong> 4~6m 2 was cleaned up by the cows to provi<strong>de</strong> a convenient and<br />

safe parturition area for calves. Calv<strong>in</strong>g was from 7 o’clock <strong>in</strong> the<br />

morn<strong>in</strong>g to 6 o’clock <strong>in</strong> the afternoon, tend<strong>in</strong>g towards a sort of<br />

daylight <strong>de</strong>liver<strong>in</strong>g system. Activity of calv<strong>in</strong>g took 63.33±101.16m<strong>in</strong><br />

after chas<strong>in</strong>g other animal and ly<strong>in</strong>g on the ground for calv<strong>in</strong>g,<br />

25.00±5.00m<strong>in</strong>. From calf born to placenta expelled, it took<br />

11.74±10.89h. The number of gett<strong>in</strong>g up and ly<strong>in</strong>g down of cows<br />

were 7.00±6.00 times dur<strong>in</strong>g the <strong>de</strong>livery course. Average birthweight<br />

was 17.83±1.04kg for male and female without any dystocia case. <br />

Activity time of new calf: From birth to the first stand<strong>in</strong>g, it took<br />

52.70±29.69m<strong>in</strong>; from the first stand<strong>in</strong>g to the first milk suck<strong>in</strong>g,<br />

38.7±29.14m<strong>in</strong> and from the first milk suck<strong>in</strong>g to walk<strong>in</strong>g around,<br />

61.33±1.53 m<strong>in</strong>. The study <strong>in</strong>dicated that calv<strong>in</strong>g performance of<br />

gayal was or<strong>de</strong>rly processed with hardly undystocia case, good<br />

maternity, better ability of rear<strong>in</strong>g calf, and the best capability of<br />

reproduction and survival <strong>in</strong> the wild condition.<br />

Keywords System of Semi-<strong>in</strong>tensive management; Mithun(Bos<br />

frontalis); calv<strong>in</strong>g behavior; <strong>in</strong>vestigation.<br />

P577<br />

Treatment of postpartum dairy cows ‘not-<strong>de</strong>tected <strong>in</strong><br />

oestrus’ with gonadotroph<strong>in</strong> releas<strong>in</strong>g hormone,<br />

prostagland<strong>in</strong> and progesterone<br />

McDougall, S<br />

Animal Health Centre, New Zealand<br />

Oestradiol benzoate (ODB) has been extensively used <strong>in</strong> synchrony<br />

programmes <strong>in</strong> dairy cattle to manage follicle wave emergence and<br />

tim<strong>in</strong>g of oestrus. However, ODB has recently been banned by the<br />

European Union and hence removed from other markets. Thus<br />

alternate protocols to synchronise cows are required. The objective of<br />

this study was to assess the efficacy of 3 treatments of dairy cows ‘not<br />

<strong>de</strong>tected <strong>in</strong> oestrus’ before the start of seasonal breed<strong>in</strong>g programme<br />

(PSM). The ovaries of 2222 cows not <strong>de</strong>tected <strong>in</strong> oestrus by 9 days<br />

before the PSM were exam<strong>in</strong>ed us<strong>in</strong>g ultrasonography and randomly<br />

assigned to be treated with: 1. Gonadotroph<strong>in</strong> releas<strong>in</strong>g hormone<br />

(GnRH), prostagland<strong>in</strong> F2a and GnRH at 7 and 2-day <strong>in</strong>tervals,<br />

respectively; with fixed time artificial <strong>in</strong>sem<strong>in</strong>ation 1 day after the<br />

second GnRH treatment (‘GPG’ programme), 2. ‘GPG’ and <strong>in</strong>sertion<br />

of an <strong>in</strong>travag<strong>in</strong>al progesterone-releas<strong>in</strong>g <strong>de</strong>vice between the first<br />

GnRH <strong>in</strong>jection and the PG, with fixed time artificial <strong>in</strong>sem<strong>in</strong>ation 1<br />

day after the second GnRH treatmen (GPG + P4) , 3. As for 2, but<br />

with oestrus <strong>de</strong>tection for 3 days after the PG treatment, and with the<br />

second GnRH treatment occurr<strong>in</strong>g only where oestrus has not been<br />

<strong>de</strong>tected by 3 days after PG (GPG + P4 + heat), or 4. No treatment<br />

(Control). Pregnancy status was assessed us<strong>in</strong>g ultrasonography at<br />

35 days after the PSM. Data were analysed us<strong>in</strong>g logistic regression<br />

mo<strong>de</strong>ls which <strong>in</strong>clu<strong>de</strong>d treatment group, age, herd, CL status<br />

(presence or absence) and days from calv<strong>in</strong>g to the PSM. Estimated<br />

marg<strong>in</strong>al means (95% CI) were calculated and the multiple<br />

comparisons among treatment groups accounted for by us<strong>in</strong>g the<br />

Bonferroni adjustment. The conception rate to service with<strong>in</strong> the first<br />

7 days was higher <strong>in</strong> the treatment groups which <strong>in</strong>clu<strong>de</strong>d<br />

progesterone than <strong>in</strong> the control or GPG groups (0.43 (0.36-0.50),<br />

0.56 (0.49-0.63), 0.48 (0.41-0.55), 0.34 (0.23-0.44) for the GPG,<br />

GPG+P4, GPG+P4+heat and Control groups, respectively; p = 0.48,<br />

0.001, and 0.04 compared to the Control group). Additionally, the<br />

pregnancy rate (i.e. total number of cows confirmed pregnant/total<br />

number of cows treated) varied among groups (0.40 (0.34-0.47), 0.54<br />

(0.47-0.60), 0.46 (0.40-0.54), 0.08 (0.05-0.11) for the GPG, GPG+P4,<br />

GPG+P4+heat and Control groups, respectively. The control was<br />

lower than all other groups (p

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