Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 216 Poster Abstracts Poster 28 – Other topics P570 Opinions and regulations of breed registries in Europe on stud book registration of cloned horses Aurich, J 1 *, Knauss, L 2 , Aurich, C 2 1Animal Breeding and Reproduction, University of Veterinary Sciences, Austria; 2 Graf Lehndorff Institute for Equine Science, Neustadt (Dosse), Germany Cloning of production farm animals so far is virtually irrelevant for breeding programmes. In contrast, horses with high breeding value are cloned with the goal to produce offspring and contribute to genetic progress. Of special interest is the cloning of geldings successful in equestrian sports in order to produce offspring from copies obtained by somatic cell nuclear transfer. The use of cloning requires acceptance of this biotechnology by the regulations of breed registries, i.e. stud book registration of cloned horses and their offspring. We have conducted a survey on the regulations of European breed registries for cloned horses and the opinion of the persons responsible for breeding programmes. A total of 34 questionaires were returned from 8 countries. None of the breed registries have so far registered cloned horses. Only two registries have specific regulations for cloned horses while 29 have not (no answer: 3). With current regulations, 5 breeds would register cloned horses (4 in Germany, one in Austria) and 5 registries are planning changes in the regulations. There are no regulations on validation of cloned status. Registry managers considered cloning mainly a technique for conservation of endangered horse breeds (82%) and less to obtain clones from elite mares (44%), stallions (33%) or geldings (37%) or to obtain copies from individual horses with high emotional value for the owner (4%). Out of the persons asked, only few thought that cloning will contribute to genetic progress (15%) but most expected cloning to help maintaining genetic material in rare breeds (78%). Out of the registry managers, one was of the opinion that performances tests of the „parent“ should be valid for the clone, 30 expected clones to undergo performance testing, 23 agreed that cloned horses should be admitted to equestrian competitions. Ethical objections against assisted reproductive technologies were raised in 15% and against cloning in 35% of the questionaires, only 19% had objections against cloning for animal welfare reasons. When asked on the opinion of their members on cloning, 77% of breed managers expected a rejection of cloning and 13% classified their members as slightly positive, 52% answered that their members were sceptical but interested in current developments on cloning. In conslusion, a board acceptance of cloning by sport horse breed registries is unlikely. There are no major ethical concerns but cloning is not expected to contribute to genetic progress. This biotechnology appears to be more justified for breeding programmes in rare breeds. P571 The effect of addition of vitamin B12 in the extender on the post-thaw motility, acrosome morphology and plasma membrane integrity in the bull semen Hu, JH 1 , Chen, YL 1 *, Li, QW 1,2 , Jia, YH 3 , Zhu, DN 3 , Wang, LQ 1 1College of Animal Science and Technology, Northwest A & F University, Yangling, ShaanXi Province 712100, P.R. China; 2 College of Environment and Chemistry Engineering, YanShan University,Qinhuangdao, HeBei Province 066004, P.R. China; 3 Domestic Animal Improving Station in ShaanXi Province, Jingyang, shaanXi 713702, P.R. China *Corresponding author. Yu-Lin Chen. Present address: College of Animal Science and Technology, Northwest A & F University, Yangling, ShaanXi Province 712100, P.R. China, Tel.:+86 29 87092102; Fax.: +86 29 87092164; E-mail address: myxy11@263.net in the extender, as compared to the control. The results indicated that the motility and VSL, VCL, STR, VAP values of sperm supplemented with 2.50 mg/ml vitamin B 12 were significantly higher than that of other concentrations (p0.05). However, vitamin B 12 significantly decreased sperm motion characteristics and the percentage of grade a spermatozoa at a concentration of 5.00 mg/ml in extender. The percentages of acrosome-intact and plasma membraneintact spermatozoa was significantly improved (p0.05). Therefore, the data were pooled for both cycles and reported as a single data set. New follicle wave emerged on Day 3-4. This follicle reached to 9.51± 0.47 mm in diameter on Day 7 after GnRH treatment. Corpus luteum was detected at the size of 14.86±0.58 (12-17.3) mm on Day 5 after induction of ovulation. Corpus luteum continued to grow until Day 7 when it reached to maximum size of 16.81±0.84 (12-21) mm. Progesterone concentrations initiated to increase on Day 5 and reached to maximum concentrations of 1.12±0.14 ng/ml on Day 7. In conclusion, steroid treatment (2 mg estradiol benzoate + 100 mg progesterone), injected on Day 2 after induction of ovulation, did not have any effect on follicle development, CL formation and progesterone concentrations in Bactrian camel. Key words Bactrian camel; Steroids; GnRH; Follicle development; Progesterone To investigate the effects of supplementation of vitamin B 12 on bovine sperm post-thaw motility and quality, it was added at concentrations of 1.25, 2.50, 3.75 and 5.00 mg/ml to bovine semen cryoprotective medium. Analysis of results showed that the motility and the primary motion characteristics were improved in the presence of vitamin B 12
16 t h International Congress on Animal Reproduction Poster Abstracts 217 P573 Sperm morphology of beef bulls evaluated by nigrosineosin and differential interference phase contras Freneau, G 1 *, Chenoweth, P 2 , Ellis, RW 3 , Rupp, G 4 1Lab. Andrologyand Semen Technology Animal Sc, Veterinary school / Federal University of Goias, Brazil; 2 School of Agricultural and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia; 3 Dept. of Clinical Sciences Integrated Livestock Ma, Colorado State University, United States; 4 Great Plains Veterinary Educational Center, University of Nebraska, United States The objectives of this study were to compare two different methods of evaluating bull sperm morphology: bright-field (BF) microscopy of eosin-nigrosin (EN) stained dry-mount semen smears and differential interference phase contrast (DIC) microscopy of wet-mount semen ‘fixed’ in isotonic formal saline; both at 1000X. Seventy-two ejaculates were evaluated, representing both pre- and post-breeding season ejaculates collected from 40 2-yr. old beef bulls via electroejaculation. For both methods, 200 sperm were counted in random fields with defects categorized as major (MAD) and minor (MID). Sperm abnormalities were also placed into two other categories: those considered to be most influenced by process (wet or dry; METHD) and those whose depiction could be influenced by optics (BF or DIC; OPTD). Differences (P0.05) in percent normal sperm 69.1 / 70.4 or sperm head defects 7.5 / 8.3. Acrosome, tail and droplet defects were observed in 98.2 / 80.5, 86.1 / 100 and 98.2 / 94.4 percent of bulls for DIC and BF respectively (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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