Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 217<br />
P573<br />
Sperm morphology of beef bulls evaluated by nigros<strong>in</strong>eos<strong>in</strong><br />
and differential <strong>in</strong>terference phase contras<br />
Freneau, G 1 *, Chenoweth, P 2 , Ellis, RW 3 , Rupp, G 4<br />
1Lab. Andrologyand Semen Technology Animal Sc, Veter<strong>in</strong>ary school /<br />
Fe<strong>de</strong>ral University of Goias, Brazil; 2 School of Agricultural and Veter<strong>in</strong>ary<br />
Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia; 3 Dept. of<br />
Cl<strong>in</strong>ical Sciences Integrated Livestock Ma, Colorado State University, United<br />
States; 4 Great Pla<strong>in</strong>s Veter<strong>in</strong>ary Educational Center, University of Nebraska,<br />
United States<br />
The objectives of this study were to compare two different methods of<br />
evaluat<strong>in</strong>g bull sperm morphology: bright-field (BF) microscopy of<br />
eos<strong>in</strong>-nigros<strong>in</strong> (EN) sta<strong>in</strong>ed dry-mount semen smears and differential<br />
<strong>in</strong>terference phase contrast (DIC) microscopy of wet-mount semen<br />
‘fixed’ <strong>in</strong> isotonic formal sal<strong>in</strong>e; both at 1000X. Seventy-two<br />
ejaculates were evaluated, represent<strong>in</strong>g both pre- and post-breed<strong>in</strong>g<br />
season ejaculates collected from 40 2-yr. old beef bulls via electroejaculation.<br />
For both methods, 200 sperm were counted <strong>in</strong> random<br />
fields with <strong>de</strong>fects categorized as major (MAD) and m<strong>in</strong>or (MID).<br />
Sperm abnormalities were also placed <strong>in</strong>to two other categories: those<br />
consi<strong>de</strong>red to be most <strong>in</strong>fluenced by process (wet or dry; METHD)<br />
and those whose <strong>de</strong>piction could be <strong>in</strong>fluenced by optics (BF or DIC;<br />
OPTD). Differences (P0.05) <strong>in</strong> percent normal sperm 69.1 /<br />
70.4 or sperm head <strong>de</strong>fects 7.5 / 8.3. Acrosome, tail and droplet<br />
<strong>de</strong>fects were observed <strong>in</strong> 98.2 / 80.5, 86.1 / 100 and 98.2 / 94.4<br />
percent of bulls for DIC and BF respectively (P