Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
216 Poster Abstracts
Poster 28 – Other topics
P570
Opinions and regulations of breed registries in Europe on
stud book registration of cloned horses
Aurich, J 1 *, Knauss, L 2 , Aurich, C 2
1Animal Breeding and Reproduction, University of Veterinary Sciences,
Austria; 2 Graf Lehndorff Institute for Equine Science, Neustadt (Dosse),
Germany
Cloning of production farm animals so far is virtually irrelevant for
breeding programmes. In contrast, horses with high breeding value are
cloned with the goal to produce offspring and contribute to genetic
progress. Of special interest is the cloning of geldings successful in
equestrian sports in order to produce offspring from copies obtained
by somatic cell nuclear transfer. The use of cloning requires
acceptance of this biotechnology by the regulations of breed
registries, i.e. stud book registration of cloned horses and their
offspring. We have conducted a survey on the regulations of European
breed registries for cloned horses and the opinion of the persons
responsible for breeding programmes. A total of 34 questionaires
were returned from 8 countries. None of the breed registries have so
far registered cloned horses. Only two registries have specific
regulations for cloned horses while 29 have not (no answer: 3). With
current regulations, 5 breeds would register cloned horses (4 in
Germany, one in Austria) and 5 registries are planning changes in the
regulations. There are no regulations on validation of cloned status.
Registry managers considered cloning mainly a technique for
conservation of endangered horse breeds (82%) and less to obtain
clones from elite mares (44%), stallions (33%) or geldings (37%) or
to obtain copies from individual horses with high emotional value for
the owner (4%). Out of the persons asked, only few thought that
cloning will contribute to genetic progress (15%) but most expected
cloning to help maintaining genetic material in rare breeds (78%). Out
of the registry managers, one was of the opinion that performances
tests of the „parent“ should be valid for the clone, 30 expected clones
to undergo performance testing, 23 agreed that cloned horses should
be admitted to equestrian competitions. Ethical objections against
assisted reproductive technologies were raised in 15% and against
cloning in 35% of the questionaires, only 19% had objections against
cloning for animal welfare reasons. When asked on the opinion of
their members on cloning, 77% of breed managers expected a
rejection of cloning and 13% classified their members as slightly
positive, 52% answered that their members were sceptical but
interested in current developments on cloning. In conslusion, a board
acceptance of cloning by sport horse breed registries is unlikely.
There are no major ethical concerns but cloning is not expected to
contribute to genetic progress. This biotechnology appears to be more
justified for breeding programmes in rare breeds.
P571
The effect of addition of vitamin B12 in the extender on the
post-thaw motility, acrosome morphology and plasma
membrane integrity in the bull semen
Hu, JH 1 , Chen, YL 1 *, Li, QW 1,2 , Jia, YH 3 , Zhu, DN 3 , Wang, LQ 1
1College of Animal Science and Technology, Northwest A & F University,
Yangling, ShaanXi Province 712100, P.R. China; 2 College of Environment
and Chemistry Engineering, YanShan University,Qinhuangdao, HeBei
Province 066004, P.R. China; 3 Domestic Animal Improving Station in
ShaanXi Province, Jingyang, shaanXi 713702, P.R. China
*Corresponding author. Yu-Lin Chen. Present address: College of Animal
Science and Technology, Northwest A & F University, Yangling, ShaanXi
Province 712100, P.R. China, Tel.:+86 29 87092102; Fax.: +86 29
87092164; E-mail address: myxy11@263.net
in the extender, as compared to the control. The results indicated that
the motility and VSL, VCL, STR, VAP values of sperm supplemented
with 2.50 mg/ml vitamin B 12 were significantly higher than that of
other concentrations (p0.05). However, vitamin B 12
significantly decreased sperm motion characteristics and the
percentage of grade a spermatozoa at a concentration of 5.00 mg/ml in
extender. The percentages of acrosome-intact and plasma membraneintact
spermatozoa was significantly improved (p0.05). Therefore, the data were
pooled for both cycles and reported as a single data set. New follicle
wave emerged on Day 3-4. This follicle reached to 9.51± 0.47 mm in
diameter on Day 7 after GnRH treatment. Corpus luteum was detected
at the size of 14.86±0.58 (12-17.3) mm on Day 5 after induction of
ovulation. Corpus luteum continued to grow until Day 7 when it
reached to maximum size of 16.81±0.84 (12-21) mm. Progesterone
concentrations initiated to increase on Day 5 and reached to maximum
concentrations of 1.12±0.14 ng/ml on Day 7. In conclusion, steroid
treatment (2 mg estradiol benzoate + 100 mg progesterone), injected
on Day 2 after induction of ovulation, did not have any effect on
follicle development, CL formation and progesterone concentrations
in Bactrian camel.
Key words Bactrian camel; Steroids; GnRH; Follicle development;
Progesterone
To investigate the effects of supplementation of vitamin B 12 on bovine
sperm post-thaw motility and quality, it was added at concentrations
of 1.25, 2.50, 3.75 and 5.00 mg/ml to bovine semen cryoprotective
medium. Analysis of results showed that the motility and the primary
motion characteristics were improved in the presence of vitamin B 12