Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
216 Poster Abstracts<br />
Poster 28 – Other topics<br />
P570<br />
Op<strong>in</strong>ions and regulations of breed registries <strong>in</strong> Europe on<br />
stud book registration of cloned horses<br />
Aurich, J 1 *, Knauss, L 2 , Aurich, C 2<br />
1Animal Breed<strong>in</strong>g and <strong>Reproduction</strong>, University of Veter<strong>in</strong>ary Sciences,<br />
Austria; 2 Graf Lehndorff Institute for Equ<strong>in</strong>e Science, Neustadt (Dosse),<br />
Germany<br />
Clon<strong>in</strong>g of production farm animals so far is virtually irrelevant for<br />
breed<strong>in</strong>g programmes. In contrast, horses with high breed<strong>in</strong>g value are<br />
cloned with the goal to produce offspr<strong>in</strong>g and contribute to genetic<br />
progress. Of special <strong>in</strong>terest is the clon<strong>in</strong>g of geld<strong>in</strong>gs successful <strong>in</strong><br />
equestrian sports <strong>in</strong> or<strong>de</strong>r to produce offspr<strong>in</strong>g from copies obta<strong>in</strong>ed<br />
by somatic cell nuclear transfer. The use of clon<strong>in</strong>g requires<br />
acceptance of this biotechnology by the regulations of breed<br />
registries, i.e. stud book registration of cloned horses and their<br />
offspr<strong>in</strong>g. We have conducted a survey on the regulations of European<br />
breed registries for cloned horses and the op<strong>in</strong>ion of the persons<br />
responsible for breed<strong>in</strong>g programmes. A total of 34 questionaires<br />
were returned from 8 countries. None of the breed registries have so<br />
far registered cloned horses. Only two registries have specific<br />
regulations for cloned horses while 29 have not (no answer: 3). With<br />
current regulations, 5 breeds would register cloned horses (4 <strong>in</strong><br />
Germany, one <strong>in</strong> Austria) and 5 registries are plann<strong>in</strong>g changes <strong>in</strong> the<br />
regulations. There are no regulations on validation of cloned status.<br />
Registry managers consi<strong>de</strong>red clon<strong>in</strong>g ma<strong>in</strong>ly a technique for<br />
conservation of endangered horse breeds (82%) and less to obta<strong>in</strong><br />
clones from elite mares (44%), stallions (33%) or geld<strong>in</strong>gs (37%) or<br />
to obta<strong>in</strong> copies from <strong>in</strong>dividual horses with high emotional value for<br />
the owner (4%). Out of the persons asked, only few thought that<br />
clon<strong>in</strong>g will contribute to genetic progress (15%) but most expected<br />
clon<strong>in</strong>g to help ma<strong>in</strong>ta<strong>in</strong><strong>in</strong>g genetic material <strong>in</strong> rare breeds (78%). Out<br />
of the registry managers, one was of the op<strong>in</strong>ion that performances<br />
tests of the „parent“ should be valid for the clone, 30 expected clones<br />
to un<strong>de</strong>rgo performance test<strong>in</strong>g, 23 agreed that cloned horses should<br />
be admitted to equestrian competitions. Ethical objections aga<strong>in</strong>st<br />
assisted reproductive technologies were raised <strong>in</strong> 15% and aga<strong>in</strong>st<br />
clon<strong>in</strong>g <strong>in</strong> 35% of the questionaires, only 19% had objections aga<strong>in</strong>st<br />
clon<strong>in</strong>g for animal welfare reasons. When asked on the op<strong>in</strong>ion of<br />
their members on clon<strong>in</strong>g, 77% of breed managers expected a<br />
rejection of clon<strong>in</strong>g and 13% classified their members as slightly<br />
positive, 52% answered that their members were sceptical but<br />
<strong>in</strong>terested <strong>in</strong> current <strong>de</strong>velopments on clon<strong>in</strong>g. In conslusion, a board<br />
acceptance of clon<strong>in</strong>g by sport horse breed registries is unlikely.<br />
There are no major ethical concerns but clon<strong>in</strong>g is not expected to<br />
contribute to genetic progress. This biotechnology appears to be more<br />
justified for breed<strong>in</strong>g programmes <strong>in</strong> rare breeds.<br />
P571<br />
The effect of addition of vitam<strong>in</strong> B12 <strong>in</strong> the exten<strong>de</strong>r on the<br />
post-thaw motility, acrosome morphology and plasma<br />
membrane <strong>in</strong>tegrity <strong>in</strong> the bull semen<br />
Hu, JH 1 , Chen, YL 1 *, Li, QW 1,2 , Jia, YH 3 , Zhu, DN 3 , Wang, LQ 1<br />
1College of Animal Science and Technology, Northwest A & F University,<br />
Yangl<strong>in</strong>g, ShaanXi Prov<strong>in</strong>ce 712100, P.R. Ch<strong>in</strong>a; 2 College of Environment<br />
and Chemistry Eng<strong>in</strong>eer<strong>in</strong>g, YanShan University,Q<strong>in</strong>huangdao, HeBei<br />
Prov<strong>in</strong>ce 066004, P.R. Ch<strong>in</strong>a; 3 <strong>Domestic</strong> Animal Improv<strong>in</strong>g Station <strong>in</strong><br />
ShaanXi Prov<strong>in</strong>ce, J<strong>in</strong>gyang, shaanXi 713702, P.R. Ch<strong>in</strong>a<br />
*Correspond<strong>in</strong>g author. Yu-L<strong>in</strong> Chen. Present address: College of Animal<br />
Science and Technology, Northwest A & F University, Yangl<strong>in</strong>g, ShaanXi<br />
Prov<strong>in</strong>ce 712100, P.R. Ch<strong>in</strong>a, Tel.:+86 29 87092102; Fax.: +86 29<br />
87092164; E-mail address: myxy11@263.net<br />
<strong>in</strong> the exten<strong>de</strong>r, as compared to the control. The results <strong>in</strong>dicated that<br />
the motility and VSL, VCL, STR, VAP values of sperm supplemented<br />
with 2.50 mg/ml vitam<strong>in</strong> B 12 were significantly higher than that of<br />
other concentrations (p0.05). However, vitam<strong>in</strong> B 12<br />
significantly <strong>de</strong>creased sperm motion characteristics and the<br />
percentage of gra<strong>de</strong> a spermatozoa at a concentration of 5.00 mg/ml <strong>in</strong><br />
exten<strong>de</strong>r. The percentages of acrosome-<strong>in</strong>tact and plasma membrane<strong>in</strong>tact<br />
spermatozoa was significantly improved (p0.05). Therefore, the data were<br />
pooled for both cycles and reported as a s<strong>in</strong>gle data set. New follicle<br />
wave emerged on Day 3-4. This follicle reached to 9.51± 0.47 mm <strong>in</strong><br />
diameter on Day 7 after GnRH treatment. Corpus luteum was <strong>de</strong>tected<br />
at the size of 14.86±0.58 (12-17.3) mm on Day 5 after <strong>in</strong>duction of<br />
ovulation. Corpus luteum cont<strong>in</strong>ued to grow until Day 7 when it<br />
reached to maximum size of 16.81±0.84 (12-21) mm. Progesterone<br />
concentrations <strong>in</strong>itiated to <strong>in</strong>crease on Day 5 and reached to maximum<br />
concentrations of 1.12±0.14 ng/ml on Day 7. In conclusion, steroid<br />
treatment (2 mg estradiol benzoate + 100 mg progesterone), <strong>in</strong>jected<br />
on Day 2 after <strong>in</strong>duction of ovulation, did not have any effect on<br />
follicle <strong>de</strong>velopment, CL formation and progesterone concentrations<br />
<strong>in</strong> Bactrian camel.<br />
Key words Bactrian camel; Steroids; GnRH; Follicle <strong>de</strong>velopment;<br />
Progesterone<br />
To <strong>in</strong>vestigate the effects of supplementation of vitam<strong>in</strong> B 12 on bov<strong>in</strong>e<br />
sperm post-thaw motility and quality, it was ad<strong>de</strong>d at concentrations<br />
of 1.25, 2.50, 3.75 and 5.00 mg/ml to bov<strong>in</strong>e semen cryoprotective<br />
medium. Analysis of results showed that the motility and the primary<br />
motion characteristics were improved <strong>in</strong> the presence of vitam<strong>in</strong> B 12