Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 201<br />
P525<br />
Laparoscopic ovum pick-up (OPU) <strong>in</strong> goat and sheep<br />
Wieczorek, J*, Kosenyuk, Y, Rynska, B; Cegla, M<br />
Department of Biotechnology of Animal <strong>Reproduction</strong>, National Research<br />
Institute of Animal Production, Krakowska 1, Balice/Kraków Poland<br />
New techniques for repeated, m<strong>in</strong>imal-<strong>in</strong>vasive oocyte recovery <strong>in</strong><br />
liv<strong>in</strong>g donor <strong>in</strong>clud<strong>in</strong>g goat and sheep are necessary. The employment<br />
of laparoscopy and vi<strong>de</strong>osurgery allowed for the work<strong>in</strong>g out the OPU<br />
methods. However, their use is restricted by the relatively low<br />
efficacy and reproducibility of the results. The aim of the study was to<br />
<strong>de</strong>velop new techniques for repeated recovery of goat and sheep<br />
oocytes useful for culture and fertilization <strong>in</strong> vitro and clon<strong>in</strong>g and<br />
evaluation of the efficacy of the established method. Oocytes were<br />
aspirated with orig<strong>in</strong>ally <strong>de</strong>signed catheter for aspiration. The oocytes<br />
donors were 65 goats and 45 sheep. Estrus was synchronized with<br />
<strong>in</strong>travag<strong>in</strong>al sponges (Chronogest CR, Intervet) for 14 days.<br />
Superovulation was obta<strong>in</strong>ed by the s<strong>in</strong>gle <strong>in</strong>jection of eCG (1000 IU<br />
IM) 16 - 24 hours before the removal of the sponges. Oocytes were<br />
collected 24 hours after sponge removal. The animals were<br />
premedicated, then general anesthesia was <strong>in</strong>duced. The general<br />
anesthesia lasted about 15 – 20 m<strong>in</strong>. The endoscope was <strong>in</strong>serted <strong>in</strong>to<br />
the abdom<strong>in</strong>al cavity through umbilicus. Two trockars for putt<strong>in</strong>g the<br />
manipulators were <strong>in</strong>serted 15 cm below the ud<strong>de</strong>r. Oocytes were<br />
collected by aspiration of the follicular fluid from the ovarian<br />
follicles. Depend<strong>in</strong>g on the size, the s<strong>in</strong>gle aspiration of up to 8<br />
follicles was performed. The collected oocytes were evaluated un<strong>de</strong>r<br />
stereo microscope. The follow<strong>in</strong>g classification of the oocytes were<br />
established: class I – homogenous cytoplasm, at least 3 layers of the<br />
granulosa cells, class II – homogenous cytoplasm, 1-2 layers of<br />
granulosa cells, class III – homogenous cytoplasm, no granulosa cells,<br />
class IV – heterogenous cytoplasm, <strong>in</strong><strong>de</strong>pen<strong>de</strong>nt of the granulosa<br />
cells. Oocytes class I, II and III were qualified for the culture. Goats:<br />
488 ovarian follicles were aspirated, 276 (56.6%; p