Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
Poster Abstracts 201
P525
Laparoscopic ovum pick-up (OPU) in goat and sheep
Wieczorek, J*, Kosenyuk, Y, Rynska, B; Cegla, M
Department of Biotechnology of Animal Reproduction, National Research
Institute of Animal Production, Krakowska 1, Balice/Kraków Poland
New techniques for repeated, minimal-invasive oocyte recovery in
living donor including goat and sheep are necessary. The employment
of laparoscopy and videosurgery allowed for the working out the OPU
methods. However, their use is restricted by the relatively low
efficacy and reproducibility of the results. The aim of the study was to
develop new techniques for repeated recovery of goat and sheep
oocytes useful for culture and fertilization in vitro and cloning and
evaluation of the efficacy of the established method. Oocytes were
aspirated with originally designed catheter for aspiration. The oocytes
donors were 65 goats and 45 sheep. Estrus was synchronized with
intravaginal sponges (Chronogest CR, Intervet) for 14 days.
Superovulation was obtained by the single injection of eCG (1000 IU
IM) 16 - 24 hours before the removal of the sponges. Oocytes were
collected 24 hours after sponge removal. The animals were
premedicated, then general anesthesia was induced. The general
anesthesia lasted about 15 – 20 min. The endoscope was inserted into
the abdominal cavity through umbilicus. Two trockars for putting the
manipulators were inserted 15 cm below the udder. Oocytes were
collected by aspiration of the follicular fluid from the ovarian
follicles. Depending on the size, the single aspiration of up to 8
follicles was performed. The collected oocytes were evaluated under
stereo microscope. The following classification of the oocytes were
established: class I – homogenous cytoplasm, at least 3 layers of the
granulosa cells, class II – homogenous cytoplasm, 1-2 layers of
granulosa cells, class III – homogenous cytoplasm, no granulosa cells,
class IV – heterogenous cytoplasm, independent of the granulosa
cells. Oocytes class I, II and III were qualified for the culture. Goats:
488 ovarian follicles were aspirated, 276 (56.6%; p