Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 200 Poster Abstracts development. The present study investigated in vitro effects of adding all-trans retinol to media containing fertilizing and developing embryos of mouse. Methods The mice were maintained according to guidelines of the committee at laboratory animals of Razi Institute. Oocytes were produced by the superovulating 150, six week old NMRI female mice. Sperm was obtained from the cauda epididims of 4, four month old NMRI male mice. In first experiment, collected mouse oocytes were fertilized in concentration 0 (control), 1, 5 and 10 micromole of retinol in HTF medium and were transferred to CZB culture medium. In second experiment, fertilized mouse embryos were cultured in concentration 0 (control), 1, 5 and 10 micromole of all-trans retinol in CZB medium. Embryos were evaluated to blastocyst stage every day. The data analysis was carried out by using proc MIXED of SAS and the Duncan’s multiple-range test was used to determine differences among means. Results In the 1 st experiment, addition of 1 or 5 micromole of retinol improved (P
16 t h International Congress on Animal Reproduction Poster Abstracts 201 P525 Laparoscopic ovum pick-up (OPU) in goat and sheep Wieczorek, J*, Kosenyuk, Y, Rynska, B; Cegla, M Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, Balice/Kraków Poland New techniques for repeated, minimal-invasive oocyte recovery in living donor including goat and sheep are necessary. The employment of laparoscopy and videosurgery allowed for the working out the OPU methods. However, their use is restricted by the relatively low efficacy and reproducibility of the results. The aim of the study was to develop new techniques for repeated recovery of goat and sheep oocytes useful for culture and fertilization in vitro and cloning and evaluation of the efficacy of the established method. Oocytes were aspirated with originally designed catheter for aspiration. The oocytes donors were 65 goats and 45 sheep. Estrus was synchronized with intravaginal sponges (Chronogest CR, Intervet) for 14 days. Superovulation was obtained by the single injection of eCG (1000 IU IM) 16 - 24 hours before the removal of the sponges. Oocytes were collected 24 hours after sponge removal. The animals were premedicated, then general anesthesia was induced. The general anesthesia lasted about 15 – 20 min. The endoscope was inserted into the abdominal cavity through umbilicus. Two trockars for putting the manipulators were inserted 15 cm below the udder. Oocytes were collected by aspiration of the follicular fluid from the ovarian follicles. Depending on the size, the single aspiration of up to 8 follicles was performed. The collected oocytes were evaluated under stereo microscope. The following classification of the oocytes were established: class I – homogenous cytoplasm, at least 3 layers of the granulosa cells, class II – homogenous cytoplasm, 1-2 layers of granulosa cells, class III – homogenous cytoplasm, no granulosa cells, class IV – heterogenous cytoplasm, independent of the granulosa cells. Oocytes class I, II and III were qualified for the culture. Goats: 488 ovarian follicles were aspirated, 276 (56.6%; p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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