Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 194 Poster Abstracts re-suspended in fresh medium, motility was evaluated and sperm concentration was determined. The suspension volume was adjusted in order to obtain 4 samples with a final concentrations of 0.025, 0.1, 0.5, 1 x10 6 msp/ml, and supplemented with PHE 20 μl/ml and heparine 10 μg/ml. Matured oocytes were co-cultured in 100 μl droplets of the different IVF suspensions under mineral oil for 18 h at 38.5°C in 5% CO 2 . After fertilization, cumulus cells were removed and presumptive zygotes were cultured in SOFaa plus BSA 16 mg/ml at 38.5 °C in 5% CO 2 . Cleavage was determined after 12 h of IVC and embryos were cultured in the same medium supplemented with 10% FBS until day 9. Results Cleavage rate, morulae-blastocyst rate on day 6 and blastocyst rate on day 9 were lower (p
16 t h International Congress on Animal Reproduction Poster Abstracts 195 cell complexes (OGCs) on the viability and the meiotic competence of in vitro grown oocytes. In this study, we assessed the morphology of OGCs on the antrum formation of OGCs, the viability and the meiotic competence of in vitro grown oocytes. Porcine OGCs were recovered from early antral follicles with diameter of 300-700 μm and intact oocytes with intact granulosa cells were selected. OGCs categorized into 3 categories according to the quantity of granulose cells attached with oocytes (Category A: 1-3 layers of granulose cells (GCs); B: 3 or more layers of GCs attached with a small amount of theca cells; C: 3 or more layers of GCs surrounded with theca cells). OGCs were cultured for 14 days in the medium (containing 2% polyvynilpyrrolidone, estradiol-17β, ascorbic acid, Insulin, sodium selenite, transferring and BSA, Hashimoto et al., 2007) supplemented with 0.02% (w/v) L-Carnitine under 5% oxygen tension. In vitro maturation (IVM) of in vitro grown oocytes was carried out for 44 hours following the culture. The antrum formation rate of OGCs in category A (18%) was significantly lower (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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