Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
Poster Abstracts 191
2007) supplemented with 0, 0.002, 0.02, and 0.2% (w/v) L-Car. In
vitro maturation of in vitro grown oocytes was carried out for 44
hours following the culture. Apoptosis indexes of granulose cells
cultured in 0 or 0.02 % (w/v) L-Car were assessed using In situ Cell
Detection Kit (Roche Diagnostics, Tokyo). To assess the cytchrome c
oxidase activity on electron microscopy, the ultrathin sections of the
in vitro grown and in vivo grown OGCs were observed by TEM. The
proportions of oocytes survived, GVBD and matured were calculated
based on the number of OGCs cultured. Data were analyzed by
Fisher’s PLSD test following ANOVA. Proportions of apoptotic cells
were compared by t test. When OGCs were cultured in 0.02% L-Car,
proportions of oocytes survived after 14-days culture, GVBD and
matured after IVM were 43%, 32%, and 25%, respectively. These
values were significantly higher (P