Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 190 Poster Abstracts with 2 pronuclei and no visible sperm; oocytes with 2 pronuclei and one sperm; oocytes with 1 pronucleus and one sperm; others, oocytes with other nuclear structures, such as more than 2 pronuclei or no analyzable oocytes. Oocytes injected with TX sperm pretreated and caffeine during and after micromanipulation had a lower oocyte activation rate than the other experimental groups (25.4% vs. 54.4- 69.1%; P
16 t h International Congress on Animal Reproduction Poster Abstracts 191 2007) supplemented with 0, 0.002, 0.02, and 0.2% (w/v) L-Car. In vitro maturation of in vitro grown oocytes was carried out for 44 hours following the culture. Apoptosis indexes of granulose cells cultured in 0 or 0.02 % (w/v) L-Car were assessed using In situ Cell Detection Kit (Roche Diagnostics, Tokyo). To assess the cytchrome c oxidase activity on electron microscopy, the ultrathin sections of the in vitro grown and in vivo grown OGCs were observed by TEM. The proportions of oocytes survived, GVBD and matured were calculated based on the number of OGCs cultured. Data were analyzed by Fisher’s PLSD test following ANOVA. Proportions of apoptotic cells were compared by t test. When OGCs were cultured in 0.02% L-Car, proportions of oocytes survived after 14-days culture, GVBD and matured after IVM were 43%, 32%, and 25%, respectively. These values were significantly higher (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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