Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
188 Poster Abstracts<br />
with r 2 = 0.97. The values of L P were <strong>de</strong>term<strong>in</strong>ed to be 0.79 ± 0.26,<br />
0.82 ± 0.22, and 0.64 ± 0.16 μm⋅m<strong>in</strong> -1 ⋅atm -1 and the P CPA values were<br />
<strong>de</strong>term<strong>in</strong>ed to be 2.9 ± 1.3, 2.7 ± 1.3, and 0.27 ± 0.18 x10 -3 cm⋅m<strong>in</strong> -1<br />
for DMSO, EG and GLY respectively. There were no significant<br />
differences (p>0.05) between values for L P and P CPA <strong>in</strong> presence of the<br />
DMSO and EG. However, these values were significantly different<br />
from the values <strong>in</strong> presence of GLY.<br />
Conclusions Similar to human oocytes, rabbit oocytes behave as i<strong>de</strong>al<br />
osmometers <strong>in</strong> the range osmolalities tested with values for V bp nearly<br />
i<strong>de</strong>ntical to human oocytes. However, rabbit oocyte isotonic volume is<br />
smaller than human oocytes. Previously published mean values for L p<br />
<strong>in</strong> human oocytes falls with<strong>in</strong> the 95% confi<strong>de</strong>nce limits for rabbit<br />
oocytes; the same is true for the permeability to DMSO. Rabbit<br />
oocytes are more permeable to EG <strong>in</strong> comparison to human oocytes,<br />
however. Higher P CPA values for rabbit oocytes result <strong>in</strong> less volume<br />
excursions than those for human oocyte dur<strong>in</strong>g CPA addition and<br />
dilution. Supported by Wellcome Trust (Grant No.070246), EU FP6<br />
(MEXT-CT-2003-509582, MRTN-CT-2006-035468) and Ch<strong>in</strong>ese-<br />
Hungarian Bilateral projects (TET CHN-28/04, CHN-41/05).<br />
P487<br />
Different response of Bos <strong>in</strong>dicus Vs Bos taurus oocyte<br />
on maturation, cleavage and embryo <strong>de</strong>velopment un<strong>de</strong>r<br />
<strong>in</strong> vitro system<br />
Escalona, F*; Mercado, J; Rodríguez, A; Rodríguez-Sallaberry, C; Kowalski, AA<br />
Laboratorio <strong>de</strong> Embriología y Endocr<strong>in</strong>ología Molecular, Decanato <strong>de</strong><br />
Agronomía, Universidad Centrocci<strong>de</strong>ntal Lisandro Alvarado (UCLA), Lara,<br />
Venezuela<br />
The <strong>in</strong> vitro of production embryos represents an alternative to the<br />
cattle <strong>in</strong>dustry for generat<strong>in</strong>g large numbers of F1 embryos. The<br />
objective of this study was <strong>de</strong>term<strong>in</strong>e the differences of <strong>in</strong> vitro<br />
maturation, fertilization and culture (IVM, IVF, IVC) of two oocytes<br />
groups; Brahman (Br) and Holste<strong>in</strong> (Ho) oocytes. Ovaries from<br />
slaughterhouse were transported <strong>in</strong> sal<strong>in</strong>e 0.9% at 30 ±1.1°C; complex<br />
oocytes-cumulus (COCs) were collected from Br (52) and Ho (51)<br />
ovaries and used for IVM, IVF and IVC. The number of oocytes from<br />
each ovary were: Br (13.65±1.3) Ho (7.30±3) respectively. COCs<br />
were cultured on TCM-199 supplemented with BSA, piruvate, L-<br />
glutam<strong>in</strong>e, FSH, LH y EGF dur<strong>in</strong>g 22 hrs, and <strong>in</strong>cubated at 38°C on<br />
5% CO 2 <strong>in</strong> a humidified environment. The COCs were transferred to<br />
IVF-TALP supplemented with hepar<strong>in</strong>, penicillam<strong>in</strong>e, hypotaur<strong>in</strong> and<br />
epynephr<strong>in</strong>e. The fertilization was ma<strong>de</strong> with sperm from Ho bulls.<br />
The semen was separated by percoll gradient and washed <strong>in</strong> spermtalp.<br />
In both groups the semen concentration was 1 x 10 6 sperm/mL<br />
and <strong>in</strong>cubated for 18 hrs. The zygotes were <strong>in</strong>cubated for 7 days <strong>in</strong><br />
KSOM supplemented with BSA, L-glutam<strong>in</strong>e, EAA and NEAA. The<br />
cleavage rate was Br (92.36±0.8%) and Ho (69.4±18.7%) (P