Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
184 Poster Abstracts<br />
P475<br />
Effect of <strong>de</strong>nsity gradient and s<strong>in</strong>gle layer centrifugation<br />
on motility and survival of boar spermatozoa<br />
Wallgren, M*, Saravia, F; Rodriguez-Mart<strong>in</strong>ez, H; Morrell, JM<br />
Dept. of Cl<strong>in</strong>ical Science, Div. of <strong>Reproduction</strong>, SLU, Uppsala, Swe<strong>de</strong>n<br />
Density gradient centrifugation was compared with centrifugation on<br />
a s<strong>in</strong>gle layer of colloid for prepar<strong>in</strong>g boar spermatozoa, us<strong>in</strong>g<br />
species-specific colloid formulations <strong>de</strong>veloped at SLU. Ejaculates<br />
(12) from four boars were exten<strong>de</strong>d 1:1 with Beltsville thaw<strong>in</strong>g<br />
solution (BTS) with<strong>in</strong> 15 m<strong>in</strong>utes of collection. The sperm<br />
concentration was adjusted to 100 million per mL, aga<strong>in</strong> with BTS;<br />
the sperm suspensions were used either immediately or after overnight<br />
storage at room temperature. Aliquots (1.5 mL) of the exten<strong>de</strong>d semen<br />
were layered on a <strong>de</strong>nsity gradient of silane-coated silica colloid<br />
formulations (2 mL of a high <strong>de</strong>nsity and 2 mL low <strong>de</strong>nsity colloid<br />
formulations) or a s<strong>in</strong>gle layer (4 ml) of the high <strong>de</strong>nsity colloid<br />
formulation, before centrifugation at 300 g for 20 m<strong>in</strong>utes. The sperm<br />
pellet was subsequently washed <strong>in</strong> BTS by centrifugation at 500 g for<br />
10 m<strong>in</strong>utes and resuspen<strong>de</strong>d <strong>in</strong> 1.0 mL BTS. Sperm motility of<br />
<strong>in</strong>cubated aliquots (37°C for 30 m<strong>in</strong>) of the sperm suspensions was<br />
assessed subjectively every day. Sperm motility was significantly<br />
better (P0.05) of CL counts among Treatments A (8.9 ± 1.3), B (11.4 ±<br />
1.3) and C (12.0 ± 1.1), respectively. The total number of recovered<br />
structures from Treatment A (6.9 ± 1.5) was no different from<br />
Treatments B (5.7 ± 1.5) and C (9.8 ± 1.2). Transferable embryos<br />
from Treatments A (2.4 ± 0.7) and B (1.7 ± 0.6) were lower (P