Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
184 Poster Abstracts
P475
Effect of density gradient and single layer centrifugation
on motility and survival of boar spermatozoa
Wallgren, M*, Saravia, F; Rodriguez-Martinez, H; Morrell, JM
Dept. of Clinical Science, Div. of Reproduction, SLU, Uppsala, Sweden
Density gradient centrifugation was compared with centrifugation on
a single layer of colloid for preparing boar spermatozoa, using
species-specific colloid formulations developed at SLU. Ejaculates
(12) from four boars were extended 1:1 with Beltsville thawing
solution (BTS) within 15 minutes of collection. The sperm
concentration was adjusted to 100 million per mL, again with BTS;
the sperm suspensions were used either immediately or after overnight
storage at room temperature. Aliquots (1.5 mL) of the extended semen
were layered on a density gradient of silane-coated silica colloid
formulations (2 mL of a high density and 2 mL low density colloid
formulations) or a single layer (4 ml) of the high density colloid
formulation, before centrifugation at 300 g for 20 minutes. The sperm
pellet was subsequently washed in BTS by centrifugation at 500 g for
10 minutes and resuspended in 1.0 mL BTS. Sperm motility of
incubated aliquots (37°C for 30 min) of the sperm suspensions was
assessed subjectively every day. Sperm motility was significantly
better (P0.05) of CL counts among Treatments A (8.9 ± 1.3), B (11.4 ±
1.3) and C (12.0 ± 1.1), respectively. The total number of recovered
structures from Treatment A (6.9 ± 1.5) was no different from
Treatments B (5.7 ± 1.5) and C (9.8 ± 1.2). Transferable embryos
from Treatments A (2.4 ± 0.7) and B (1.7 ± 0.6) were lower (P