Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 177<br />
Methods A total of eight cl<strong>in</strong>ically normal, sexually mature twoyears-old<br />
dairy-Gyr bulls (25.7 ± 0.8 months old) with average score<br />
84.4 ± 15.6 po<strong>in</strong>ts <strong>in</strong> the CAP evaluation (zero to 100 scale), scrotal<br />
circumference 34.4 ± 2.5, body weight 354.7 ± 26.3 Kg and scanned<br />
for andrological features from 18,2 ± 0.8 months old had their semen<br />
collected by eletroejaculation. The bulls were raised, from wean<strong>in</strong>g to<br />
21.2 ± 0.8 months old, un<strong>de</strong>r tropical pastures of Brachiaria brizhanta<br />
receiv<strong>in</strong>g m<strong>in</strong>eral salt supplementation and from there they were kept<br />
<strong>in</strong>dividually <strong>in</strong> a feed<strong>in</strong>g-lot un<strong>de</strong>r full diet management based <strong>in</strong> corn<br />
silage and 27% cru<strong>de</strong> prote<strong>in</strong> / 83% total digestible nutrients ration.<br />
Sperm Motility (MOT) and vigor (VIG) were immediately analyzed.<br />
The semen was diluted <strong>in</strong> both EYL and Bioxcell ® diluters until 60.0<br />
x 10 6 sptz/mL and loa<strong>de</strong>d <strong>in</strong> 0.5 mL straws at room temperature be<strong>in</strong>g<br />
stored at 5ºC for a cool<strong>in</strong>g stabilization period from four to six hours.<br />
The negative curve was performed by the Brazilian mach<strong>in</strong>e<br />
CRYOGEN ® until -135ºC, when the straws were immersed <strong>in</strong> liquid<br />
nitrogen. A total of 160 straws were evaluated (10 from each bull per<br />
exten<strong>de</strong>r used) for MOT and VIG post-thaw<strong>in</strong>g and also for the Host<br />
and compared by the SNK test (p