Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 176 Poster Abstracts P450 Factors affecting pregnancy rates following fixed-time AI in beef cattle in Argentina Cutaia, L 1 *, Lopez, P 2 , Videla Dorna, I 2 , Leblic, D 2 , Castagneto, N 2 , Diaz, T 2 , Bo, GA 1 1Instituto de Reproduccion Animal Cordoba (IRAC), Argentina; 2 Syntex SA, Argentina Field data on 54,457 inseminations in 396 herds were analyzed to determine the factors that affected pregnancy rates following fixedtime AI (FTAI) of beef cattle in Argentina between 2004 and 2006. Cattle were treated by 39 different veterinarians with a new or onceused progesterone releasing device (1.0 g progesterone; DIB, Syntex, Argentina) for 7 or 8 days, combined with 2 mg estradiol benzoate (EB, Syntex) at DIB insertion, 150 µg D(+) cloprostenol (Ciclase, Syntex) at DIB removal and 1 mg EB 24 h later. Cattle were FTAI 52 to 56 hours after DIB removal. Lactating beef cows in poor body condition also received 400 IU eCG (Novormon, Syntex) at DIB removal. Pregnancy rates were determined 30 to 60 days following FTAI by ultrasonography or rectal palpation. Data were analyzed by logistic regression. The overall pregnancy rate was 49.5%. Body condition score was not included in the model because of inconsistencies in methodology and scoring among veterinarians. Breed significantly influenced pregnancy rates (P
16 t h International Congress on Animal Reproduction Poster Abstracts 177 Methods A total of eight clinically normal, sexually mature twoyears-old dairy-Gyr bulls (25.7 ± 0.8 months old) with average score 84.4 ± 15.6 points in the CAP evaluation (zero to 100 scale), scrotal circumference 34.4 ± 2.5, body weight 354.7 ± 26.3 Kg and scanned for andrological features from 18,2 ± 0.8 months old had their semen collected by eletroejaculation. The bulls were raised, from weaning to 21.2 ± 0.8 months old, under tropical pastures of Brachiaria brizhanta receiving mineral salt supplementation and from there they were kept individually in a feeding-lot under full diet management based in corn silage and 27% crude protein / 83% total digestible nutrients ration. Sperm Motility (MOT) and vigor (VIG) were immediately analyzed. The semen was diluted in both EYL and Bioxcell ® diluters until 60.0 x 10 6 sptz/mL and loaded in 0.5 mL straws at room temperature being stored at 5ºC for a cooling stabilization period from four to six hours. The negative curve was performed by the Brazilian machine CRYOGEN ® until -135ºC, when the straws were immersed in liquid nitrogen. A total of 160 straws were evaluated (10 from each bull per extender used) for MOT and VIG post-thawing and also for the Host and compared by the SNK test (p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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