Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
172 Poster Abstracts<br />
P438<br />
Correlaction between bov<strong>in</strong>e sperm membrane <strong>in</strong>tegrity<br />
and mitochondrial cytochemical activity <strong>in</strong> Bos taurus<br />
bulls<br />
Zuge, RM 1,2 ; Bertolla, RP 3 ; Nichi, M 1,4 ; Soler, TBS 1,3 ; Cortada, CNM 2 *; Bols,<br />
PEJ 4 ; Barnabe, VH 1<br />
1Department of Animal <strong>Reproduction</strong> (VRA), Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e<br />
and Zootechny (FMVZ), University of São Paulo (USP), Brazil; 2 Paraná<br />
Technology Institute (Tecpar), Brazil; 3 Department of Human <strong>Reproduction</strong>,<br />
Fe<strong>de</strong>ral University of São Paulo (UNIFESP), Brazil; 4 Laboratory of Veter<strong>in</strong>ary<br />
Physiology, Faculty of Pharmaceutical, Biomedical and Veter<strong>in</strong>ary Sciences,<br />
University of Antwerp, Belgium<br />
Introduction Previous studies with varicocele <strong>in</strong> men and European<br />
bulls <strong>in</strong> tropical region, <strong>in</strong>dicated that heat stress would <strong>in</strong>duce<br />
<strong>de</strong>leterious effects to all structures of the spermatozoa, with special<br />
regard to the mitochondria. The disruption of the spermatic<br />
mitochondria could have a potential damag<strong>in</strong>g effect not only to an<br />
<strong>in</strong>dividual cell but to the surround<strong>in</strong>g cells, specially regard<strong>in</strong>g to the<br />
sperm membrane. This could be due to the release of a high amount of<br />
reactive oxygen species (ROS) produced <strong>in</strong> this environment, rich <strong>in</strong><br />
electrons and oxygen that would lead to an event known as lipid<br />
peroxidation.<br />
Methods To study whether sperm cells with disrupted mitochondria,<br />
would show higher amount of membrane damages probably due to<br />
higher levels of ROS attack, semen samples of eleven heat stressed<br />
Simmental bulls were collected by electroejaculation, the method of<br />
choice when work<strong>in</strong>g un<strong>de</strong>r field conditions <strong>in</strong> Brazil. The sta<strong>in</strong><strong>in</strong>g 3-<br />
3’ diam<strong>in</strong>o benzid<strong>in</strong>e (DAB), as an <strong>in</strong><strong>de</strong>x of mitochondrial activity,<br />
the hypo-osmotic swell<strong>in</strong>g test (HOST), as an <strong>in</strong><strong>de</strong>x of membrane<br />
<strong>in</strong>tegrity, and the measurement of tiobarbituric acid reactive<br />
substances (TBARS), an <strong>in</strong><strong>de</strong>x of spontaneous lipid peroxidation were<br />
used. Data was analysed us<strong>in</strong>g the SAS system for W<strong>in</strong>dows.<br />
Results Results showed a positive correlation between sperm cells<br />
with full mitochondrial activity (DAB class A), and percentage of<br />
cells with <strong>in</strong>tact membrane by the HOST (r=0.93, p