Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 170 Poster Abstracts P432 The effect of the addition of seminal plasma and antioxidants to frozen thawed ram semen Sicherle, CC 1 *, Maia, MS 2 , Bicudo, SD 1 , Green, RE 1 , Azevedo, HC 2 1Department of Animal Reproduction and Veterinary Radiology, UNESP, Botucatu, São Paulo, Brazil; 2 Embrapa Semi Árido, Petrolina, Pernambuco, Brazil, d Embrapa Tabuleiros Costeiros, Sergipe, Alagoas, Brazil Intoduction The structural changes in ram spermatozoa after cryopreservation are a fact assumed by several authors (1). This fact can be explained by a combination of factors that include a low level of membrane phospholipids and also by the oxidation process that generate the reactive oxygen species (ROS) which, when in high levels, are toxic to spermatic cells (2). The seminal plasma (SP) is a complex mixture of components as proteins, sugars and antioxidants. Evidence of high levels of pregnancy after cervical insemination in ewes with frozen thawed semen after a swim-up procedure supplemented with SP indicates a way to improve fertility using this method for artificial insemination in sheep (3). The aim of this study was to evaluate the effect of the addition of SP and the antioxidants catalase (CAT) and Trolox (TRO) on the structural and kinetics parameters on frozen thawed ram semen, independent of individual characteristics of cryopreservation resistance. Materials and Methods With this purpose four groups were established, CO (semen sample + 200µL PBS), CAT (12,5mg/mL+ PBS = 200µL), TRO (100µMol/100 x 10 6 sptz + PBS = 200µL) and SP (60% of seminal plasma diluted in PBS) added to semen samples in the proportion of 1:1. The SP was obtained from tree different rams, after semen collection all ejaculates were pooled, centrifuged and filtered as described by Mortimer & Maxwell (4). Semen samples were obtained from 13 rams and after dilution for a final concentration of 100 x 10 6 sptz/0,25mL were frozen. One sample from with ram per group were thawed and immediately mixed on the solutions groups for posterior analyses after 5 minutes of incubation. The kinematics parameters, as total motility, progressive motility, average path velocity, curvilinear velocity and straight-line velocity were analyzed using the computer assisted sperm analyzer (CASA). The membrane integrity (MI) were determined by by the combination of fluorescent probes Propidium Iodide and Carboxyflorescein, and on the capacitation status analyzed by the assay using Chortetracycline (CTC) (4). Results and Discution There were no statistic differences (P>0,05) between groups on the kinematics parameters, on the membrane integrity, and on the capacitation status analyzed by the assay using Chortetracycline (CTC). Probably in our experimental conditions the oxidative stress generated wasn’t high enough to permit the effectiveness of the antioxidants. P433 Semen quality (SQ) and scrotal circumference (SC), in Nelore bulls, from 2 to 6 years old Silva, PAR. 1 *, Emerick, LL. 1 , Martins, JAM. 1 , Andrade, VJ. 1 , Quintao Lana, AM. 1 , Leite, TG. 1 ,Vale Filho, VR. 1 , Salamanca, E. 2 1Medicine Veterinary School, Federal University of Minas Gerais, Brazil; 1Universidad de Ciencias Aplicadas Y ambientales, UDCA, Bogota, Colombia Introduction SC and SQ are important parameters for selecting bulls with adequated reproductive efficiency during the first 21 days of breeding season. However, there are still some doubts related to bull age in relation to semen quality, as far as reproductive efficiency is concerned (Feliciano Silva et al., 1993). The aim of this study was to evaluate the evolution curve of SC and total semen defects (TD) from 2 to 6 year-old Nelore bulls, elucidating doubts concerning SQ as the animal ages. Material and Methods Semen samples from 163 Nelore bulls, aging from 2 to 6 years, were collected by electro ejaculation and evaluated according to Brazilian College of Animal Reproduction (1998). Models of linear and quadratic regression were estimated (Sampaio, 2002), verifying the evolution of TD and SC according to age. Results A linear model (Y=0.59-0.53X; R²=0.92, p
16 t h International Congress on Animal Reproduction Poster Abstracts 171 P435 Potential fertility estimation in bulls using polyacrylamide gel instead of cervical mucus in the sperm penetration test Taş, M 1 *, Bacinoglu, S 2 , Cirit, Ü 2 , Özgümüş, S 3 , Kaşgöz, H 3 , Pabuccuoglu, S 2 1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dicle University, 21280 Diyarbakir, Turkey; 2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey; 3 Sub Department of Chemical Technologies, Department of Chemical Engineering, Faculty of Engineering, Istanbul University, 34320 Istanbul, Turkey The aim of the study was to develop a polyacrylamide gel that could be used instead of bovine cervical mucus in the cervical mucus penetration test (CMPT) to obtain coherent and replicable results in bulls. The frozen semen samples of six Holstein bulls, which were divided into two fertility groups as high and low according to their non-return rate (NRR), were used. The modified CMPT (mCMPT) was carried out within 0.25 ml transparent plastic straws with an inner diameter 1.7 mm in this study. The penetration ability of spermatozoa to bovine cervical mucus and to polyacrylamide gels swollen with two different solutions [NaCl (G1) and PBS (G2)] was compared. For the penetration test, the straws filled with cervical mucus and both gels were dipped into thawed semen samples and incubated at 37 °C for 15 min. After the incubation, straws were frozen in liquid nitrogen vapour and stored at -20 °C. On the evaluation day, the frozen straws were cut at 1.5–1.75 cm (penetration distance range = PDR1), 3.25– 3.5 cm (PDR2) and 5.0–5.25 cm (PDR3), beginning from open-end of the straws. The separated frozen parts were then immediately transferred onto special counting slides by pushing with a mandrel and left to thaw. Thawed samples were covered with cover glass and penetrated spermatozoa in these parts were counted. The relation between the results and fertility of bulls was determined. When compared with the low fertility group, the number of penetrating spermatozoa was found to be significantly higher in the high fertility group in mucus at PDR3 (p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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