Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 168 Poster Abstracts P426 Phenotypic correlations among andrologic markers of Nelore bulls from two to six years old, raised under pasture condition at Minas Gerais state, Brazil Nogueira, LAG. 1 *; Fragua, JD 2 ; Salamanca, E 2 ; Andrade, VJ. 1 ; Martins, JAM 1 ; Emerick, LL. 1 ; Souza, FA. 1 Vale Filho, VR. 1 ¹Veterinary Medicine School, Federal University of Minas Gerais, Brazil; ²University of Applied and Environmental Sciences, UDCA, Bogota, Colombia Introduction Scrotal Circumference (SC), physical and morphological semen characteristics, expressed by Breeding Soundness Evaluation for Zebu (BSE-Z) index, can be taken as reference for evaluation of bulls, since sexual maturity phase. The aim of this study was to estimate phenotypic correlations among andrological markers of Nelore bulls from 2 to 6 years old. Material and Methods A total of 163 Nelore bulls from 2 to 6 years old were evaluated according to CBRA (1998) and classified by BSE- Z according to Vale Filho et al. (1988). Pearson’s and Spearman’s correlations were estimated by CORR procedure using SAS (2002) with 5% of significance. Results High correlations of BSE-Z, motility and total sperm defects (TD) were, respectively, 0.65 and -0.67 (P
16 t h International Congress on Animal Reproduction Poster Abstracts 169 factors such as: blood, urine, bacteria, or debris that possibly lead to reduced fertility. In dogs, blood is known to negatively affect semen preservation. Improving the quality of contaminated semen by purification prior to preservation or fertilization may increase the overall success rate in terms of pregnancy. The density gradient centrifugation method has been demonstrated as simple, inexpensive, and results in favorable sperm cell recovery. Our aim was to compare different commercial media [Isolate®, Percoll®, PureCeption®, PureSperm®] in their ability to optimally separate viable, motile sperm from non-motile sperm and red blood cells (RBC). Methods The sperm-rich 2nd fractions of 4 dogs were pooled. The pooled semen was analyzed with Spermvision for concentration, motility, morphology, and diluted to 300 x106/ml with CaniPro® chilled extender. Canine whole blood (10% v/v) was added to semen to mimic contamination. One ml of the blood/sperm admixture was pipetted over 4 ml of a double layered-column of the following gradients: Isolate®, Percoll®, PureCeption®, and PureSperm®. After centrifugation at 400 x g for 20 min at 23°C, the gradients were divided into 1 ml fractions (Top, B, C, D, E, Pellet). The 1 ml fractions were analyzed with Spermvision. Morphology of the Dif Quik stained smears of the fractions were examined under light microscopy (1000X). For the RBC/sperm ratio, 100 cells were counted in three fields in each slide, and an average was obtained. Results The fraction with the highest concentration of motile sperm and the lowest ratio of RBC for each media is a follows: PureCeption® pellet; PureSperm® E; Percoll® D, pellet; and Isolate® D, pellet. The percent recovery of sperm cells from the fraction with the highest concentration in each media was as follows: PureCeption® (93.0±2.25%) concentration, (72.0±12.97%) motility; PureSperm® (44.8±25.9%) concentration, (53.5±17.11%) motility; Percoll® (31.0±22.48%) concentration, (70.39±32.10%) motility; Isolate® (49.67±41.37%) concentration, (59.7±21.38%) motility. PureCeption® had a significantly higher percent recovery of motile sperm cells compared to the other sperm separation media (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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