Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

More documents

Recommendations

Info

$(Microsoft PowerPoint - Lecci\363n 2_07.ppt)$

16 t h International Congress on Animal Reproduction 162 Poster Abstracts seminiferous tubules (ST), percentual and absolute (AVST) volumes of seminiferous epithelium and testicular interstitium, and number of Sertoli cells/ST cross-section and /testis, diameter of epididymal duct, of epididymal duct lumen, epithelium height of epididymal duct (EHED). Epididymal variables were studied for caput, corpus and cauda epididymal regions. Results (mean±sd) were compared with t tests (p≤0,05). Selected Pearson correlations were studied. Mother body weight (BW) at weaning (g, U vs C: 258.0±25.3***, 361.9±33.1), pup’s BW at slaughter (254.0±27.0***, 342.4±10.2), paired testicular weight (2.7±0.3*; 3.0±0.2), paired epididymal weight (g, 1.1±0.0**, 1.2±0.1), number of Sertoli cells/ST cross-section (18.2±1.2**, 20.2±1.3) and /testis (30.5±4.2x10 6 *, 36.0±5.4x10 6 ) were smaller in group U. Mother BW at weaning was correlated with pup’s BW at slaughter (0.86***), testicular weight (0.72***), AVST (0.73***) and number of Sertoli cells/testis (0.69**). Pup’s BW at slaughter was correlated with testicular weight (0.65**), AVST (0.99***) and number of Sertoli cells/testis (0.64**). Testicular weight was correlated with number of Sertoli cells/testis (0.61**). Only EHED (corpus region) was different between groups (µm, U vs. C: 31.3± 4.4** vs 17.05± 7.8). Epididymal duct diameter from caput and corpus regions (0.039*), and from caput and cauda regions (0.035*) were correlated. The EHED (corpus region) was correlated with maternal BW at weaning (0.059*), pup’s BW at slaughter (0.059*), testicular weight (0.041*) and EHED (caput region, 0.014*). In conclusion, testicular structure, in particular the number of Sertoli cells/testis (and consequently, maximal sperm production) in adult rats is affected by undernutrition during fetal to prepubertal life. Epididymides from such animals are lighter but show minor changes in histological structure. P408 Cryopreservation of ovine semen with different techniques and cryoprotectors Carneiro, GF 1,2 *; Maia, VN 2 ; Silva, SV 1,3 ; Gomes Neto, OC 1,2 ; Procópio, OCS 1 ; Medeiros, LRD 1,2 1Caroatá Genética, Gravatá, PE, Brazil; 2 Clinica de Eqüinos Pedro Zaluski LTDA, Recife, PE, Brazil; 3 Universidade Federal Rural de Pernambuco, Recife, PE, Brazil Introduction The use of Artificial Insemination (AI) associated with semen cryopreservation enable a rapid multiplication in the number of superior animals per sire and gives the possibility of storage and manipulation of genetic material. Objective: To compare the efficiency of three cryoprotectors: 7% glycerol (G), 7% ethilene glycol (EG) and 3% dimethil formamide (DF) on cryopreservation of ovine semen using two frozen techniques, conventional (with liquid nitrogen vapor in the styrofoam box) and automated (with a programmed frozen machine – TK 3000). Methods The study was performed at Caroatá Genética, Gravatá - PE, Brazil from August to November, 2006. Semen was obtained from five Santa Inês breed mature rams with 3-5 years old, with proved seminal quality. Semen collection was performed using artificial vagina with a total of six ejaculates per animal, giving a total of thirty ejaculates. Spermatozoa concentration was performed by spectrophotometry (Spermacue). After evaluation of semen sample, total volume was divided in three equal aliquotes, and diluents with each cryoprotector to be tested was added. Semen was processed in 0,25 mL straws and divided in equal numbers for each frozen technique. Straws were thawed for evaluation at least 5 days after frozen. Progressive individual motility/vigor was assessed subjectively under cover slip on a warm stage by phase contrast microscopy. Data from the variables frozen technique and motility/vigor was performed by chi-square (χ2). Results No difference were seen between the frozen techniques (p
16 t h International Congress on Animal Reproduction Poster Abstracts 163 P410 Effect of Theriogon on fertility of native bulls and buffalobulls El-Amrawi, G.A.* Faculty of Veterinary Medicine, Alexandria University Four native bulls and four buffalo-bulls (7-8 years of age) were used for investigating the effect of Theriogon on libido and fertilizing capacity. Serum and semen samples were collected for three weeks (twice per week) before treatment to serve as controls. A single oral dose of Theriogon (100 mg/ kg. body weight) was given to each animal and serum and semen samples were collected twice per week form all animals for three weeks post treatment. On collection, libido index and reaction time (in seconds) were measured and the collected semen samples were evaluated for: Ejaculate volume, pH, individual sperm motility, percentage of live sperm, sperm cell concentration (in millions/ml), total sperm number per ejaculate and sperm abnormalities (primary and secondary). Testosterone concentration was assessed in serum samples using RIA technique. The fertility of each bull was assessed before and after treatment. The results revealed that, a single oral dose of Theriogon leads to improvement in testosterone level, libido, semen quality and sperm fertilizing capacity in both bulls and buffalo-bulls. P411 Seminal plasma heparin binding protein (HBP) in Gyr bulls and its association with andrological characteristics Folhadella, I. 1 *; Camargo, LSA. 2 ; Castro TS. 1 ; Salvador, DF 3 ; Sá, WF. 2 ; Ferreira, AM. 2 ; Andrade, VJ. 1 ; Vale Filho, VR. 1 1Veterinary School of Federal University of Minas Gerais, Brazil; 2 Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil; 3 CEDERJ, Rio de Janeiro, Brazil Introduction Bulls subfertility represents a negative impact in beef and milk cattle industry. Bulls with normal breeding soundness evaluation (BSE) have different fertility profiles that may be associated with differences in seminal chemical compounds, what leads to a need to look for biochemical markers to these differences. The aim of this study was to identify heparin binding proteins (HBP) of seminal plasma and their association with andrological characteristics such as scrotal circumference, motility, vigor, major and total sperm defects, and Andrological Classification by Points. Material and Methods welve Gyr bulls were evaluated by Breeding Soundness Evaluation for Zebu (BSE-Z), according to Vale Filho (1989). A sample of 1mL of fresh semen was frozen in liquid nitrogen for purification, isolation, quantification and identification of HBP by gel filtration chromatography and chromatography affinity. The concentration was evaluated according to Lowry (1951) and HBP identification by 1-D electrophoresis, with the reader in Totallab 100 program. Statistical analyses of possible associations between BSE-Z and HBP were made by Pearson Correlation, using the SAS (2002). Results and discussion Chromatography profile of HBP showed five heparin affinity peaks. HBP concentrations varied from 0.02096 to 0.19025mg/ml. Within the five HPB peaks were found eighteen HPB with different molecular weights. From these eighteen HBP band found in electrophoresis gel, the most concentrated HPBs were those with 13, 14, 16, 18, 20, 28, 29 and 30 KDa molecular weights. No association was found among proteins and andrological parameters. Conclusions HBP evaluations were not a feasible method for predicting field andrological parameters. Its use alone does not allow consistent results for selecting high reproductive performance bulls. P412 Casa measurements of concentration, motility and viability, compared to established procedures for bull semen analysis: accuracy, reliability and its predictive value for fertility Frijters, A* R&D, CRV Holding BV, Netherlands Introduction The bull AI industry likes to use the most objective and standard semen analysis methods, that are also useful to predict fertility. The CASA instrument IVOS (Hamilton Thorne) was compared to our current tests, of which some are subjective visual microscopic assessments. Materials and methods Ejaculates (n=262) of 39 HF test bulls (12- 15 months old) were collected, processed and frozen (15 million total cells/dose) over a period of 3 months. Concentration and motility were determined of fresh and thawed semen. IVOS was compared to Coulter counting (CC) for concentration measurements, using Heamo cytometry (HC) as the golden reference. IVOS was also compared to our standard tests for motility and viability (both visual microscopic assessments). Viability was only determined for thawed semen, using IDENT/VIADENT stains for IVOS analysis, and Hoechst staining for our standard test. For IVOS analysis 4-chamber slides were used (LEJA). To evaluate reliability, Coefficients of Variation (CV) were determined by processing and measuring samples in duplicate. The predictive value of many sperm characteristics for fertility (NRR) was analysed with GENSTAT (v 8.1). Results Concentrations of fresh semen correlated highly between the used methods (R≥0.89). Values obtained by IVOS and CC respectively were 1.0 ± 16.5 (n=46) and 0.2 ± 8.2% (n=49) lower than by HC (n=49). CV ± SD were 12.0 ± 10.0 (n=252) and 2.0 ± 1.8% (n=254). Using thawed semen, IVOS and CC respectively counted 2.6 ± 10.0 (n=256) more, and 2.1 ± 8.7% (n=256) less cells, compared to the expected dose. CV ± SD were 5.9 ± 5.6 (n=256) and 4.2 ± 4.2% (n=256). Motility measured by IVOS and our standard test did not correlate when fresh or thawed semen was used. The range was higher for thawed semen using IVOS: 18-69 vs 25-55% (n=256). Viability measured by IVOS and our standard test correlated moderately (R=0.76) for thawed semen, and resulted in CV ± SD of 5.6 ± 5.8 (n=255) and 2.3 ± 1.6% (n=256) respectively. The range was higher using IVOS: 19-81 vs 41-78% (n=255). A better prediction of NRR is possible using IVOS, but we were unable to quantify this, due to an insufficient number of inseminations. Conclusions When using IVOS, CC and HC correctly, concentrations are the same. However, high reliability is easier and faster obtained by CC. IVOS and our current tests do not measure motility and viability similarly, while differences are easier detected by IVOS. More inseminations are needed to study if IVOS predicts fertility better in addition to, or instead of our current tests. P413 Recrudescence of spermatogenesis following downregulation with a GnRH-implant in the dog: first morphological and hormone-analytical results Goericke-Pesch, S 1 *; Spang, A 1 ; Bergmann, M 2 ; Hoffmann, B 1 1Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Germany; 2 Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University Giessen, Germany Aberrations in semen quality causing infertility in males must still often be classified as idiopathic as no other deviations from normal, also in respect to peripheral hormone levels, have been found. Hence not an absolute deficit but rather aberrations in the local availability of hormonal control factors may be responsible for disruption of spermatogenesis. Consequently recrudescence of spermatogenesis was monitored after having achieved downregulation of testicular function with a GnRH-implant (Gonazon®, Intervet 18.5 mg Azagly- Nafarelin) in 30 Beagles. Implant removal was after 5 months (week 0) and 3-4 dogs were castrated at weeks 0, 3, 6, 9, 12, 15, 18, 21 and 24, the testes were conserved for further examination. To assess
Page 1 and 2:
REPRODUCTION IN DOMESTIC ANIMALS 43
Page 3 and 4:
16th International Congress on Anim
Page 5 and 6:
16 t h International Congress on An
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114: 16 t h International Congress on An
Page 163: 16 t h International Congress on An
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
show all

Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?