Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
162 Poster Abstracts<br />
sem<strong>in</strong>iferous tubules (ST), percentual and absolute (AVST) volumes<br />
of sem<strong>in</strong>iferous epithelium and testicular <strong>in</strong>terstitium, and number of<br />
Sertoli cells/ST cross-section and /testis, diameter of epididymal duct,<br />
of epididymal duct lumen, epithelium height of epididymal duct<br />
(EHED). Epididymal variables were studied for caput, corpus and<br />
cauda epididymal regions. Results (mean±sd) were compared with t<br />
tests (p≤0,05). Selected Pearson correlations were studied. Mother<br />
body weight (BW) at wean<strong>in</strong>g (g, U vs C: 258.0±25.3***,<br />
361.9±33.1), pup’s BW at slaughter (254.0±27.0***, 342.4±10.2),<br />
paired testicular weight (2.7±0.3*; 3.0±0.2), paired epididymal weight<br />
(g, 1.1±0.0**, 1.2±0.1), number of Sertoli cells/ST cross-section<br />
(18.2±1.2**, 20.2±1.3) and /testis (30.5±4.2x10 6 *, 36.0±5.4x10 6 )<br />
were smaller <strong>in</strong> group U. Mother BW at wean<strong>in</strong>g was correlated with<br />
pup’s BW at slaughter (0.86***), testicular weight (0.72***), AVST<br />
(0.73***) and number of Sertoli cells/testis (0.69**). Pup’s BW at<br />
slaughter was correlated with testicular weight (0.65**), AVST<br />
(0.99***) and number of Sertoli cells/testis (0.64**). Testicular<br />
weight was correlated with number of Sertoli cells/testis (0.61**).<br />
Only EHED (corpus region) was different between groups (µm, U vs.<br />
C: 31.3± 4.4** vs 17.05± 7.8). Epididymal duct diameter from caput<br />
and corpus regions (0.039*), and from caput and cauda regions<br />
(0.035*) were correlated. The EHED (corpus region) was correlated<br />
with maternal BW at wean<strong>in</strong>g (0.059*), pup’s BW at slaughter<br />
(0.059*), testicular weight (0.041*) and EHED (caput region, 0.014*).<br />
In conclusion, testicular structure, <strong>in</strong> particular the number of Sertoli<br />
cells/testis (and consequently, maximal sperm production) <strong>in</strong> adult<br />
rats is affected by un<strong>de</strong>rnutrition dur<strong>in</strong>g fetal to prepubertal life.<br />
Epididymi<strong>de</strong>s from such animals are lighter but show m<strong>in</strong>or changes<br />
<strong>in</strong> histological structure.<br />
P408<br />
Cryopreservation of ov<strong>in</strong>e semen with different<br />
techniques and cryoprotectors<br />
Carneiro, GF 1,2 *; Maia, VN 2 ; Silva, SV 1,3 ; Gomes Neto, OC 1,2 ; Procópio,<br />
OCS 1 ; Me<strong>de</strong>iros, LRD 1,2<br />
1Caroatá Genética, Gravatá, PE, Brazil; 2 Cl<strong>in</strong>ica <strong>de</strong> Eqü<strong>in</strong>os Pedro Zaluski<br />
LTDA, Recife, PE, Brazil; 3 Universida<strong>de</strong> Fe<strong>de</strong>ral Rural <strong>de</strong> Pernambuco,<br />
Recife, PE, Brazil<br />
Introduction The use of Artificial Insem<strong>in</strong>ation (AI) associated with<br />
semen cryopreservation enable a rapid multiplication <strong>in</strong> the number of<br />
superior animals per sire and gives the possibility of storage and<br />
manipulation of genetic material. Objective: To compare the<br />
efficiency of three cryoprotectors: 7% glycerol (G), 7% ethilene<br />
glycol (EG) and 3% dimethil formami<strong>de</strong> (DF) on cryopreservation of<br />
ov<strong>in</strong>e semen us<strong>in</strong>g two frozen techniques, conventional (with liquid<br />
nitrogen vapor <strong>in</strong> the styrofoam box) and automated (with a<br />
programmed frozen mach<strong>in</strong>e – TK 3000).<br />
Methods The study was performed at Caroatá Genética, Gravatá - PE,<br />
Brazil from August to November, 2006. Semen was obta<strong>in</strong>ed from<br />
five Santa Inês breed mature rams with 3-5 years old, with proved<br />
sem<strong>in</strong>al quality. Semen collection was performed us<strong>in</strong>g artificial<br />
vag<strong>in</strong>a with a total of six ejaculates per animal, giv<strong>in</strong>g a total of thirty<br />
ejaculates. Spermatozoa concentration was performed by<br />
spectrophotometry (Spermacue). After evaluation of semen sample,<br />
total volume was divi<strong>de</strong>d <strong>in</strong> three equal aliquotes, and diluents with<br />
each cryoprotector to be tested was ad<strong>de</strong>d. Semen was processed <strong>in</strong><br />
0,25 mL straws and divi<strong>de</strong>d <strong>in</strong> equal numbers for each frozen<br />
technique. Straws were thawed for evaluation at least 5 days after<br />
frozen. Progressive <strong>in</strong>dividual motility/vigor was assessed<br />
subjectively un<strong>de</strong>r cover slip on a warm stage by phase contrast<br />
microscopy. Data from the variables frozen technique and<br />
motility/vigor was performed by chi-square (χ2).<br />
Results No difference were seen between the frozen techniques<br />
(p