Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
160 Poster Abstracts<br />
Malnourished dur<strong>in</strong>g second half of pregnancy, receiv<strong>in</strong>g 70% of their<br />
energy and prote<strong>in</strong> requirements (MN n=12); 3) Malnourished but<br />
supplemented, two weeks before parturition, with 0.6 kg of ground<br />
maize/animal (S, n=14). The follow<strong>in</strong>g variables were recor<strong>de</strong>d: 1)<br />
dystocia, 2) motor activity and head reflex response <strong>in</strong> the first hour<br />
after birth, 3) birth weight and 4) percentage of mortality dur<strong>in</strong>g the<br />
fist 45 days after birth. Proportion of dystocia was significantly higher<br />
<strong>in</strong> MN compared to S and C groups (MN: 16/29, S: 7/32 and C: 3/23<br />
kids, P0.05). After birth, kids from malnourished goats spent<br />
longer time try<strong>in</strong>g to stand up than kids from Control mothers (1380.2<br />
± 291.2 vs 464 ± 75.9 sec., P=0.009), while non significant<br />
differences were found between kids from S and MN, and those from<br />
S and C goats. Similar results were found <strong>in</strong> the kid´s latency to be<br />
completely stood up (2457.4 ± 243 vs 1461.2 ± 199.4 sec., P=0.01).<br />
Proportion of kids show<strong>in</strong>g positive response to the head reflex test<br />
(head ris<strong>in</strong>g after a touch on their nose) was smaller <strong>in</strong> MN than <strong>in</strong> C<br />
and S (MN: 7/16, C: 14/20 and S 20/27, P=0.04).While proportion of<br />
kids shak<strong>in</strong>g their head <strong>in</strong> response to tickl<strong>in</strong>g <strong>in</strong>si<strong>de</strong> their ear ten<strong>de</strong>d<br />
to be smaller <strong>in</strong> kids from MN than those from S and C mothers<br />
(P=0.08). Kids from Control were heavier at birth than those from<br />
Supplemented and Malnourished mothers (Control: 3.54 ± 0.1,<br />
Supplemented: 3.04 ± 0.9 and Malnourished: 3.02 ± 0.1 kg, P=0.01).<br />
Mortality from birth until the first 45 days of life was significantly<br />
higher <strong>in</strong> kids from MN than those from Control and Supplemented<br />
mothers (MN: 40%, C: 12% and S: 18%, P=0.05); non significant<br />
differences were found between Control and Supplemented. It is<br />
conclu<strong>de</strong>d that a high-energetic food supplementation few days before<br />
parturition improves some aspects of vitality and consequently the<br />
viability of kids from un<strong>de</strong>rfed mothers. Supported by PAPIIT<br />
IN217205, FIS B/3872-1 and CATEDRA IN2-07.<br />
P402<br />
Neonatal cl<strong>in</strong>ical evaluation of Holste<strong>in</strong> calves born un<strong>de</strong>r<br />
dist<strong>in</strong>ct obstetric conditions<br />
Rodrigues, JA; Niemeyer, C; Silva, LCG; Lúcio, CF; Veiga, GAL; Vannucchi, CI*<br />
School of Veter<strong>in</strong>ary Medic<strong>in</strong>e and Animal Sciences, University of São Paulo,<br />
Brazil<br />
Dur<strong>in</strong>g normal bov<strong>in</strong>e parturition, uter<strong>in</strong>e contractions compress<br />
umbilical cord and uter<strong>in</strong>e arteries, caus<strong>in</strong>g a reduction of fetal blood<br />
flow and aci<strong>de</strong>mia. However, dur<strong>in</strong>g dystocia, uter<strong>in</strong>e contractions are<br />
more <strong>in</strong>tensive, which even worsened these conditions.<br />
Adm<strong>in</strong>istration of oxytoc<strong>in</strong> dur<strong>in</strong>g maternal dystocia may compromise<br />
fetal well-be<strong>in</strong>g due to maternal hypotension and <strong>in</strong>creased fetal<br />
stress. The aims of this study were to evaluate neonatal blood gases,<br />
acid base and cl<strong>in</strong>ical parameters and to compare the <strong>in</strong>itial period of<br />
metabolic compensation un<strong>de</strong>r dist<strong>in</strong>ct obstetrical conditions. <strong>Animals</strong><br />
were allocated <strong>in</strong>to 3 groups: Group 1–eutocia (n=10); Group 2–fetal<br />
dystocia with obstetric assistance (n=10); Group 3–maternal dystocia<br />
treated with oxytoc<strong>in</strong> and calcium gluconate <strong>in</strong>fusion (n=4). Neonates<br />
were exam<strong>in</strong>ed us<strong>in</strong>g the APGAR scor<strong>in</strong>g and rectal temperature<br />
measurement at birth, 5 and 60 m<strong>in</strong>utes after calv<strong>in</strong>g. Arterial blood<br />
samples were collected at <strong>de</strong>livery and after 60 m<strong>in</strong>utes, <strong>in</strong> or<strong>de</strong>r to<br />
evaluate blood gases, acid base parameters, hematocrit, hemoglob<strong>in</strong>,<br />
Na, K, BUN and glucose. Calves of Group 2 showed reduced vitality<br />
as compared to the other groups. Moreover, pH (7.15±0.15) and BE (-<br />
9.1mmol/L±10.3) at birth were significantly lower than <strong>in</strong> the other<br />
groups, while HCO 3 (19.1mEq/L±8.1) was only lower than the<br />
reference values. Therefore, these results showed that dystocia can<br />
cause fetal distress as <strong>de</strong>monstrated by metabolic acidosis due to<br />
reduced blood supply to vital organs. Calves of Group 3 also showed<br />
metabolic acidosis at birth (pH 7.23±0.03 and BE -4.5mmol/L±2.1)<br />
and low pO2 at both measurements (44.5mmHg±10.6 and<br />
42.2mmHg±11.2). However, there was no difference among groups.<br />
Oxytoc<strong>in</strong> <strong>in</strong>fusion can cause dist<strong>in</strong>ct uter<strong>in</strong>e contraction pattern<br />
dim<strong>in</strong>ish<strong>in</strong>g maternal-fetal circulation and <strong>in</strong>creas<strong>in</strong>g neonatal stress.<br />
Group 3 exhibited significantly higher HCO 3 (26.3mEq/L±2.3) after 1<br />
hour than the other calves, <strong>de</strong>monstrat<strong>in</strong>g the requirement to<br />
compensate hypoxia after <strong>de</strong>livery. Dur<strong>in</strong>g asphyxia, blood flow<br />
redistributes, result<strong>in</strong>g <strong>in</strong> lower renal perfusion and ischemic renal<br />
<strong>in</strong>jury. As a result, electrolytic disturbances such as hyponatremia,<br />
hypopotassemia and uremia were observed. All newborns showed<br />
lower hematocrit and hemoglob<strong>in</strong> results than reference values due to<br />
umbilical hemorrhage or immature erytropoiesis. All calves born<br />
normally or by assistance showed evi<strong>de</strong>nt thermoregulation and<br />
glucose ma<strong>in</strong>tenance1 hour after birth. The obstetric condition was<br />
crucial to neonatal cl<strong>in</strong>ical <strong>de</strong>velopment ma<strong>in</strong>ly affect<strong>in</strong>g maternalfetal<br />
circulation <strong>in</strong> dystocias and compromis<strong>in</strong>g the newborn vitality.<br />
FAPESP 06/50485-7.<br />
Poster 16 - Andrology, Male Genitals<br />
P403<br />
Seric testosterone concentration (STC) <strong>in</strong> young Guzerat<br />
bulls (Bos taurus <strong>in</strong>dicus) and its association with<br />
reproductive traits<br />
Andra<strong>de</strong>, VJ.*; Dias, JC.; Mart<strong>in</strong>s, JAM; Emerick, LL.; Ivo, JC.,Vale Filho, VR;<br />
Silva, MA, Souza, FA.<br />
Universida<strong>de</strong> Fe<strong>de</strong>ral <strong>de</strong> M<strong>in</strong>as Gerais - Belo Horizonte, MG, Brasil<br />
Introduction Positive associations have been found (Gwasdauskas et<br />
al., 1980) among seric testosterone concentrations and reproductive<br />
characteristics, <strong>in</strong>dicative of bull fertility. This study aimed to<br />
evaluate seric testosterone levels and their associations with<br />
andrologic characteristics <strong>in</strong> young Guzerat bulls.<br />
Material and Methods Blood samples collected at two hours<br />
<strong>in</strong>tervals from 7 AM to 7 PM and reproductive characteristics from 24<br />
young Guzerat bulls, ag<strong>in</strong>g from 24 to 34 months, raised un<strong>de</strong>r<br />
pasture conditions were evaluated. STC was <strong>de</strong>term<strong>in</strong>ed by<br />
radioimmunoassay. Andrologic evaluations were performed accord<strong>in</strong>g<br />
to Brazilian College of Animal <strong>Reproduction</strong> (1998) and the animals<br />
submitted to the BSE for zebu (BSE-Z), accord<strong>in</strong>g to Vale Filho<br />
(1989). Pearson & Spearman correlations were used to estimate the<br />
associations among reproductive traits and STC.<br />
Results and discussion Means for age, weight, STC and andrologic<br />
characteristics (Scrotal circumference – SC; Sperm motility – SM;<br />
Vigor – Vig; Semen Volume – SVOL; Sperm Concentration –<br />
SCONC; Major Sperm Defects – MD; Total Sperm Defects – TD and<br />
BSE-Z. Means for age, weight, STC and reproductive characteristics<br />
of Guzerat bulls ag<strong>in</strong>g from 24 to 34 months, raised on pasture STC<br />
varied from 0.18 to 4.10ng/mL. It was also registered variation <strong>in</strong><br />
STC accord<strong>in</strong>g to blood collection time, with highest concentration<br />
(4.42 ng/mL) at 7 AM and lowest (0.36 ng/mL) at 7PM. It was also<br />
recor<strong>de</strong>d correlations (P0.05) among physical and morphological<br />
semen characteristics as reported by Gwasdauskas et al. (1980).<br />
Conclusion It was registered great variability <strong>in</strong> STC <strong>in</strong> young<br />
Guzerat bulls, suggest<strong>in</strong>g that it can be used as an auxiliary parameter<br />
<strong>in</strong> i<strong>de</strong>ntify<strong>in</strong>g bulls with greater reproductive potential, based on its<br />
favorable associations with reproductive characteristics.<br />
P404<br />
Absorptive activities <strong>in</strong> the efferent ducts of adult cat<br />
studied by Aquapor<strong>in</strong> immunohistochemistry and lect<strong>in</strong><br />
histochemistry<br />
Arrighi, S 1 *; Ventriglia, G 2 ; Aralla, M 1 ; Zizza, S 2 ; De Metrio, G 2 ; Desantis, S 2<br />
1Department of Veter<strong>in</strong>ary Science and Technologies for Food Safety, Faculty<br />
of Veter<strong>in</strong>ary Medic<strong>in</strong>e, University of Milan, Italy; 2 Department of Animal<br />
Health and Well-be<strong>in</strong>g, Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e, University of Bari, Italy<br />
Ultrastrucural features of the epithelium l<strong>in</strong><strong>in</strong>g the efferent ducts (ED)<br />
<strong>in</strong> the cat, as <strong>in</strong> other mammalian species, are strongly <strong>in</strong>dicative of an<br />
absorptive activity tak<strong>in</strong>g place towards the <strong>in</strong>tralum<strong>in</strong>al fluids. It is<br />
well-known that more than 95% of the fluid leav<strong>in</strong>g the testis is<br />
reabsorbed by the ED, but the cell structures <strong>in</strong>volved <strong>in</strong> the<br />
reabsorption processes are still a matter of <strong>de</strong>bate. The purpose of the<br />
present work was to study the absorptive pathways <strong>in</strong> the ED of adult