Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 158 Poster Abstracts P395 The effect of intra-mammary infection on 205-day adjusted weight of calves in a Brahman herd Gonzalez-Castro, F 1 *, Scaramelli, A 2 , Cordero, F 2 , Tirado, MA 3 , Diaz, T 4 , Clerc, K 3 1Departamento Medico Quirurgico, Facultad de Ciencias Veterinarias, UCV, Venezuela; 2 Patologia, Facultad de Ciencias Veterinarias, UCV, Venezuela; 3Medico Quirurgico, Facultad de Ciencias Veterinarias, UCV, Venezuela; 4Instituto De Reproduccion Animal, acultad de Ciencias Veterinarias, UCV, Venezuela In order to evaluate the effect of intra-mammary infection (IMI) on 205-day adjusted weight of calves (P205d) in a Brahman herd, 104 Brahman cows with >1 calving were used. Calving season spreads from February 15th to June 15th. Restricted suckling, from day 30 postpartum, allowed 2 hours calf-cow contact in the morning and was maintained until cow was diagnosed pregnant. Bacteriological analyses were performed to individual quarter samples during early lactation (EL; 30±7 d post-partum; n=104) and at weaning (W; 210 d post-partum; n=66). Milk samples and their duplicates were collected aseptically in sterile vials, and stored on ice for transportation to the laboratory for bacteriological analysis, 6-8 h after sampling. Staphylococcus sp. were identified using growth in blood agar (GBA) and salt manitol agar, Gram staining (GS), catalase test (CT), OF glucose test, coagulase test (CT). Streptococcus sp. were identified using GBA, GS, CT, hypurate hydrolysis, aesculin hydrolysis, growth in the presence of bile, tolerance to 6.5% NaCl and CAMP test. Gram + bacilli were identified by GBA, GC, growth in Tween 80 and in the presence of 9.5% NaCl. Frequency distribution was used to determine the IMI prevalence in cows and quarters. ANOVA was used to study the effect of IMI on P205d, and only EL data was considered. The prevalence of IMI in cows during EL and W were: Coagulase negative Staphylococcus sp. different from S. epidermidis (CNS)= 19,2% (20/104) and 15,4% (10/65); Corynebacterium sp.= 7,7% (8/104) and 4,6% (3/65); C. bovis= 3,8% (4/104) and 3,1% (2/65); S. uberis= 3,8% (4/104) and 4,6% (3/65); S. agalactiae= 1% (1/104) and 1,5% (1/65); S. hyicus= 1% (1/104) and 1,5% (1/65); S. intermedius= 1% (1/104) and 0,0% (0/65); mixed infections= 4% (4/104) and 3% (2/65), respectively. The prevalence of IMI in quarters during EL and at W were: CNS= 10,2% (40/392) and 7,2% (18/249); Corynebacterium sp.= 4,8% (19/392) and 3,6% (9/249); C. bovis= 2,6% (10/392) and 4,4% (11/249); S. uberis= 2,0% (8/392) and 1,2% (3/249); S. agalactiae= 0,3% (1/392) and 1,6% (4/249); S. intermedius= 0,5% (2/392) and 0,0% (0/249); S. hyicus= 0,0% (0/392) and 0,4% (1/249); mixed infections= 1,1% (4/392) and 0,8% (2/249), respectively. IMI had no significant effect on P205d. P205d of calves from non-infected cows was 167.23±3 kg (n=52) and from infected cows 163.05±3 kg (n=37). In conclusion, even though a significant effect of IMI on P205d was not found, numeric differences were observed, and non infected cows weaned slightly heavier calves than infected cows. P396 The effect of umbilical cord clamping on acid-base balance of piglets at term Jonker, FH 1 *, Van Dijk, J 2 , Van Loon, TPAM 3 , Taverne, MAM 1 1Farm animal Health, Faculty of Veterinary Medicine, Utrecht University, the Netherlands, Netherlands; 2 Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Netherlands; 3 Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Netherlands Umbilical cord clamping (UCC) was used to mimic the evolvement of birth asphyxia, like observed in natural farrowings. A laparatomy was conducted in 4 sows (on day 112 or 113) under general anaesthesia. Successive fetal compartments were exteriorised and each fetus was placed, with an intact umbilical cord and its head covered by a plastic bag, under a heating lamp. Piglets were alternately subjected either to 5-8 min of UCC (n=23) or served as controls (n=24) during a similar waiting period (WP). After cutting of the cord, 21 control and 21 clamped piglets had to be supported by manual ventilation with room air to establish independent respiration (IR). In 4 control and 7 clamped piglets no IR occurred and these piglets were classified as non-surviving (NSV). Mean birth weight and duration of UCC or WP of NSV piglets did not differ significantly from surviving (SV) piglets. Before UCC / WP, acid-base balance values and heart rates (HR) of piglets did not differ within and between litters throughout the 5 h of surgery (mean 291± 44 min). UCC resulted in an initial fetal bradycardia, with a consecutive gradual increase in HR, followed by a second decrease in HR at which moment UCC was stopped. HR in control piglets remained constant during the WP. Mean body weight (BW) and duration of UCC / WP did not differ between SV control piglets (n=20; BW: 1336± 417 g; WP: 7.0± 1.9 min) and SV clamped piglets (n=16; BW: 1392± 403 g; UCC: 7.0± 1.1 min). Only a mild, mixed respiratory-metabolic acidosis in umbilical artery blood was measured at 10 min after cutting of the cord in SV UCC piglets, with lower pH (7.22; range 7.10-7.32) and BE (2 mmol/L; -3 to 7) and higher pCO2 (9.8 kPa; 7.0 – 12.2) and lactate values (6.5 mmol/L; 5.4 – 8.9) compared to SV control piglets (pH: 7.31, range 7.17 – 7.38; BE: 5 mmol/L, range -6 to 10; pCO2: 8.5 kPa, range 6.8 – 12.2; lactate: 4.0 mmol/L, range 3.0 – 5.6), independently of spontaneous breathing, manual ventilation and BW. This study demonstrates that loss of umbilical cord function alone is not responsible for severe asphyxia during birth. The response to UCC was rather variable with respect to onset of the second HR decrease and the degree of acidosis. The latter was not as severe as reported by Herpin et al. in 1996 (pH < 7.00; pCO2 >11.8 kPa; lactate >7.2 mmol/L ) for highly asphyxiated, vaginally delivered piglets. P397 Biochemical profile of the amniotic fluid at the delivery moment of the Nelore calves conceived by in vitro production and embryo transfer Moya, CF 1 *; Piagentini, M 1 ; Prestes, NC 1 ; Lucidi, CA 2 ; Takahira, RK 2 1Department of Animal Reproduction and Veterinary Radiology, São Paulo State University - FMVZ/UNESP, Brazil; 2 Department of Veterinary Clinics, São Paulo State University - FMVZ/UNESP, Brazil The purpose of the present study was to quantify biochemical constituents of the amniotic fluid of the Nelore calves conceived by in vitro production and embryo transfer at the delivery moment. Forty cows divided in 2 groups were used in this experiment: 01- Twenty cows pregnant with Nelore calves obtained by in vitro production after follicular aspiration; 02 - Twenty cows pregnant with Nelore calves obtained by superovulation of embryo donors. The animals were fed on pasture with mineral salt, supplement of corn silage and ration in the rural area in Avaré, São Paulo, Brazil. Near to the labor, the cows were transferred to a maternal paddock, permitting delivery observation. During the expulsion phase the amnion was punctured and 15mL of fluid were collected, kept in a plastic tube and stored in a freezer. The evaluation of the biochemical parameters were made through commercial kits. Total protein, glucose, chloride and urea were determined by colorimetric spectrophotometry. Creatinine and gama glutamyltransferase (GGT) were determined by kinetic spectrophotometry. Potassium and sodium were determined by flame photometry. Statistics included analysis of variance and Tukey test considering 5% as the level of significance. The mean values and their standard error for the biochemical parameters obtained from the amniotic fluid in the Group 01 were: 0.45±0.46 g/dL for total protein, 4.4±6.1 mg/dL for glucose, 8.0±5.3 mg/dL for creatinin, 40.3±16.5 mg/dL for urea, 17.2±14.6 UI/L for GGT, 63.7±31.5 b mmol/L for chloride, 94.8±33.6 mmol/L for sodium and 8.3±7.1 a mmol/L for potassium. The mean values and their standard error for the biochemical parameters obtained from the amniotic fluid in the Group 02 were: 0.36±0.57 g/dL for total protein, 6.7±7.7 mg/dL for glucose, 5.9±4.9 mg/dL for creatinina, 38.3±20.5 mg/dL for urea, 22.6±13.0 UI/L for GGT, 88.8±23.8 a mmol/L for chloride, 77.7±33.4 mmol/L for sodium and 3.9±1.8 b mmol/L for potassium (p
16 t h International Congress on Animal Reproduction Poster Abstracts 159 P398 Study of pituitary-adrenal axis in the trotter newborn foal Sgorbini, M 1 ; Paci, V 2 *; Maccheroni, M 3 ; Luchetti, E 1 ; Rota, A 1 ; Marmorini, P 4 ; Corazza, M 1 1Dipartimento Clinica Veterinaria, via Livornese lato monte, 56010 San Piero a Grado (PI), Italy; 2 DVM, Florence, Italy; 3 Laboratorio Dosaggi Endocrinologici, Azienda Ospedaliera Pisana, via Bonanno Pisano, 56100 Pisa, Italy; 4 DVM, Pisa, Italy Introduction A rise in foetal plasma cortisol concentration has been detected in many species at a time when the maturational changes are occurring. A distinction between post natal cortisol levels in mature and immature foals is present. The aim of the present work is to study the trend of the plasmatic concentration of ACTH and cortisol in trotter foals during the first 48h of life. Materials and methods Fifty trotter foals different in sex and born in the same farm during breeding seasons 2006 were included in this study. Inclusion criteria: 1) 320-340 gestation days; 2) assisted deliveries; 3) mares vaccination for influenza and EHV-1 and treatment for GI parasites; 4) Apgar ≥7; 5) IgG concentration at 24h ≥800 mg/dl (SNAP ® Foal IgG, IDEXX, USA); 6) immediate suction reflex and standing the head, sternal recumbency within 3 min, quadrupedal position within 160 min, first suckling within 180 min; 7) normal physical examination during the first week of life. Blood samples were collected within 6h from birth (T1), at 24 (T2) and 48h (T3) of life in EDTA and heparinised tubes. Samples were immediately centrifuged and plasma was stored at -20°C. Both ACTH and cortisol were analysed by chemoluminescence (Immunolite 2005 Analyzer, DPC, USA). X±SD were calculated both for ACTH and cortisol. Correlation has been calculated for both the hormones in relation with sampling times. Anova test has been applied both for ACTH and cortisol vs different sampling times and the differences were considered statistically significant for p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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