Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
Poster Abstracts 155
acid supplementation can stimulate follicular dynamics and minimize
post-partum anestrous.
P386
Role of Adiponectin in Regulating Ovarian Theca and
Granulosa Cell Function in Cattle
Spicer, LJ*; Lagaly, DV; Aad, PY; Grado-Ahuir, JA; Hulsey, LB
Animal Science, Oklahoma State University, United States
Adiponectin is an adipokine that has been implicated in insulin
resistance, a condition associated with polycystic ovarian syndrome in
humans, and alters steroid production by rat and pig granulosa cells.
Furthermore, adiponectin receptor mRNA has recently been localized
within ovarian tissue of rats and chickens, but whether adiponectin
can directly affect ovarian theca or granulosa cell function in cattle is
unknown. Therefore, experiments were conducted to determine the
effects of adiponectin on proliferation, steroidogenesis and gene
expression of large-follicle theca and granulosa cells as well as
compare adiponectin receptor 2 (ADIPOR2) mRNA abundance in
theca and granulosa cells of small and large follicles. Fluorescent
real-time quantitative RT-PCR was used to elucidate the effects of
adiponectin on gene expression of side-chain cleavage enzyme
(CYP11A1) and LH receptor (LHR) in large-follicle theca and
granulosa cells, as well as expression of 17-hydroxylase (CYP17A1)
in theca cells and aromatase (CYP19A1) in granulosa cells.
Adiponectin (3 μg/ml) attenuated insulin-like growth factor-I (IGF-I)-
induced LHR, CYP11A1, and CYP17A1 gene expression in theca
cells as well as decreased (P < 0.05) LH plus insulin-induced
progesterone and androstenedione production by theca cells. In
contrast, adiponectin (3 μg/ml) decreased (P < 0.05) LHR mRNA
abundance in granulosa cells but did not affect steroidogenic enzyme
gene expression in granulosa cells. Theca cells from large (8-22 mm)
follicles had fourfold greater (P < 0.05) abundance of ADIPOR2
mRNA than theca cells from small (2-6 mm) follicles. In contrast,
granulosa cells from small and large follicles had similar (P > 0.10)
ADIPOR2 mRNA abundance and these levels did not significantly
differ from those of small-follicle theca cells. To evaluate if
hormones regulate ADIPOR2, theca cells from large follicles were
incubated in the presence of 0 or 30 ng/ml of IGF-I without or with 30
ng/ml of LH for 24 h. IGF-I decreased (P < 0.05) abundance of
ADIPOR2 mRNA by 14% in untreated theca cells but had no effect
(P > 0.10) on ADIPOR2 mRNA abundance in LH-treated largefollicle
theca cells. In contrast, LH increased (P < 0.05) theca cell
ADIPOR2 mRNA abundance by 17% and 27% in the absence and
presence of IGF-I, respectively. These results indicate that the
inhibitory effects of adiponectin on steroidogenesis are primarily
localized to theca cells and that the response system of theca cells to
adiponectin (i.e., ADIPOR2) may be regulated during follicle growth
by LH and IGF-I.
P387
Renal abscesses as a complication of ovaryhisterectomy
in a bitch
Vicente, WRR 1 *, Medeiros, MG 1 , Pereira, ML 2 , Bürger, C 2 , Voorwald, FA 1 ,
Motheo, TF 1 , Carvalho, MB 2 , Toniollo, GH 1
1Animal Reproduction, Faculty of Agriculture and Veterinary Sciences, São
Paulo State University, Brazil; 2 Veterinary Clinics and Surgery, Faculty of
Agriculture and Veterinary Sciences, São Paulo State University, Brazil
Rarely, renal abscesses are seen in dogs and can occur due to
pyelonephritis, nephrolithiasis, and kidney biopsy. The
ovaryhisterectomy (OHE) can show numerous complications such as
hemorrhage, uterine stump pyometra, pyometra, fistules, urether
ligature, hydronephrosis or pyonephrosis, urinary incontinence, partial
colon obstruction and granulomas. To date, there are no reports of
OHE complications resulting in kidney abscesses in this specie. A ten
year-old Rottweiler female was referred to the Veterinary Teaching
Hospital featuring anorexia, oligodipsia and vomiting for three days.
The bitch underwent OHE due to pyometra history three weeks ago.
Physical examination revealed prostration, moderated dehydration and
intense abdominal and lumbar pain. Laboratory findings revealed
normocytic, normochromic anemia, leukocytosis, mild azotemia, and
high levels of alkaline phosphatase. Results of the urinalysis showed
isostenuria, hematuria, leukocyturia and bacteriuria. A large round
mass appeared on abdominal radiograph as a soft tissue-density,
caudal to the right side of the liver. Abdominal ultrasound revealed a
round structure with multiple fluid-filled areas in the same region.
Laparotomy was performed for definitive diagnosis. During the
procedure, the right kidney was large, round, grey to yellowish,
irregular and adhered to the abdominal aorta. Immediately, the animal
underwent nephrectomy. There were cortical abscesses and pelvic
recesses thickening at the sagital section through the kidney. Medical
therapy included fluidtherapy for ten days, in addition to
metronidazole (15 mg/kg PO, bid), tramadol (2 mg/kg PO, tid) and
ranitidine (2.2 mg/kg PO, bid) for five days, and enrofloxacin (5
mg/kg PO, bid) for sixty days. Despite chronic renal failure, the
patient was discharged after sixty days from the surgery, and
recovered uneventfully. In summary, we hypostatized that the
abscesses formation was due to a lesion in the capsule of the kidney
that was promoted by a complication during the OHE procedure itself.
P388
Effects of endothelin-1 (ET-1) a vasoconstrictor and
bradykinin (BK) a vasodilator on luteal function in vitro in
ewes
Weems, Y 1 *; Johnson, D 1 ; Uchima, T 1 ; Raney, A 1 ; Lennon, E 1 ; Bowers, G 1 ;
Randel, R 2 ; Weems, C, 1
1Dept. of HNFAS, University of Hawaii, USA; 2 Agricultural Research and
Extension Center, Texas A&M University at Overton, USA
PGF 2 α is the uterine luteolysin delivered locally from the uterus to the
adjacent corpus luteum (CL)-containining ovary. However, cow CL
secretes PGF 2 α before the onset of luteolysis, but it also secretes the
PGE at a PGE:PGF 2 α ratio of 1:1 and PGE1 and PGE2 prevents
luteolysis (Weems et al. 2006; The Vet J:171:206-228 for review).
ET-1 has been reported to mediate PGF2α-induced luteolysis (Milvae,
Rev. Reprod. 5:1, 2000). Amounts of mRNA encoding ET-1
converting enzyme-1 (ECE-1), pre-pro ET-1, and the ET receptors
(ETA, ETB) increased in bovine luteal tissue from days 1 through day
10 postestrus, amounts on day 17 were similar to day 10, and were not
increased by PGF2α on day 10 when CL are responsive to the
luteolytic actions of PGF2α, but were increased by exogenous PGF2α
on day 17 only when luteolysis was already underway (Choudhary et
al., Domest. Anim. Endocrinol. 27:63, 2004). In addition, Nitric oxide
(NO) has been reported to be luteolytic by some in cows (Weems et
al. 2006; The Vet J:171:206-228 for review). Furthermore, ET-1 and
NO donors increased PGE secretion by bovine CL slices in vitro when
estrus was not synchronized or when estrus was synchronized with
PGF2α and did not affect CL PGF2α or progesterone secretion a . In
addition, ET-1 or NO donors infused chronically intrauterine or into
the interstitial tissue of the CL-containing ovine ovary delayed
luteolysis. Therefore, the objective of this experiment was to
determine whether ET-1 affected progesterone, PGE, or PGF2α
secretion by ovine CL. Days-12 or 16 ovine CL were collected,
weighed, and slices were incubated in vitro with Vehicle, ET-1,
Bradykinin (BK-vasodilator), Bradyzide (BZ-BK2 receptor
antagonist) or HOE-140 (BK1 receptor antagonist) at 39 C for 1 hour
without treatments and for 4 and 8 hours with treatments. Media
collected at 4 and 8 hours were analyzed for progesterone, PGE, and
PGF2α by RIA. CL weights were analyzed by a One Way ANOVA.
Hormone data were analyzed by a 2X5 Factorial Design for ANOVA.
CL weights differed (P