16 t h International Congress on Animal <strong>Reproduction</strong> 154 Poster Abstracts (BHB>1 mmol/l) the average apoptosis <strong>in</strong><strong>de</strong>x was 23,83±6,44%, it is significantly less than the result of the normoketonaemic (BHB
16 t h International Congress on Animal <strong>Reproduction</strong> Poster Abstracts 155 acid supplementation can stimulate follicular dynamics and m<strong>in</strong>imize post-partum anestrous. P386 Role of Adiponect<strong>in</strong> <strong>in</strong> Regulat<strong>in</strong>g Ovarian Theca and Granulosa Cell Function <strong>in</strong> Cattle Spicer, LJ*; Lagaly, DV; Aad, PY; Grado-Ahuir, JA; Hulsey, LB Animal Science, Oklahoma State University, United States Adiponect<strong>in</strong> is an adipok<strong>in</strong>e that has been implicated <strong>in</strong> <strong>in</strong>sul<strong>in</strong> resistance, a condition associated with polycystic ovarian syndrome <strong>in</strong> humans, and alters steroid production by rat and pig granulosa cells. Furthermore, adiponect<strong>in</strong> receptor mRNA has recently been localized with<strong>in</strong> ovarian tissue of rats and chickens, but whether adiponect<strong>in</strong> can directly affect ovarian theca or granulosa cell function <strong>in</strong> cattle is unknown. Therefore, experiments were conducted to <strong>de</strong>term<strong>in</strong>e the effects of adiponect<strong>in</strong> on proliferation, steroidogenesis and gene expression of large-follicle theca and granulosa cells as well as compare adiponect<strong>in</strong> receptor 2 (ADIPOR2) mRNA abundance <strong>in</strong> theca and granulosa cells of small and large follicles. Fluorescent real-time quantitative RT-PCR was used to elucidate the effects of adiponect<strong>in</strong> on gene expression of si<strong>de</strong>-cha<strong>in</strong> cleavage enzyme (CYP11A1) and LH receptor (LHR) <strong>in</strong> large-follicle theca and granulosa cells, as well as expression of 17-hydroxylase (CYP17A1) <strong>in</strong> theca cells and aromatase (CYP19A1) <strong>in</strong> granulosa cells. Adiponect<strong>in</strong> (3 μg/ml) attenuated <strong>in</strong>sul<strong>in</strong>-like growth factor-I (IGF-I)- <strong>in</strong>duced LHR, CYP11A1, and CYP17A1 gene expression <strong>in</strong> theca cells as well as <strong>de</strong>creased (P < 0.05) LH plus <strong>in</strong>sul<strong>in</strong>-<strong>in</strong>duced progesterone and androstenedione production by theca cells. In contrast, adiponect<strong>in</strong> (3 μg/ml) <strong>de</strong>creased (P < 0.05) LHR mRNA abundance <strong>in</strong> granulosa cells but did not affect steroidogenic enzyme gene expression <strong>in</strong> granulosa cells. Theca cells from large (8-22 mm) follicles had fourfold greater (P < 0.05) abundance of ADIPOR2 mRNA than theca cells from small (2-6 mm) follicles. In contrast, granulosa cells from small and large follicles had similar (P > 0.10) ADIPOR2 mRNA abundance and these levels did not significantly differ from those of small-follicle theca cells. To evaluate if hormones regulate ADIPOR2, theca cells from large follicles were <strong>in</strong>cubated <strong>in</strong> the presence of 0 or 30 ng/ml of IGF-I without or with 30 ng/ml of LH for 24 h. IGF-I <strong>de</strong>creased (P < 0.05) abundance of ADIPOR2 mRNA by 14% <strong>in</strong> untreated theca cells but had no effect (P > 0.10) on ADIPOR2 mRNA abundance <strong>in</strong> LH-treated largefollicle theca cells. In contrast, LH <strong>in</strong>creased (P < 0.05) theca cell ADIPOR2 mRNA abundance by 17% and 27% <strong>in</strong> the absence and presence of IGF-I, respectively. These results <strong>in</strong>dicate that the <strong>in</strong>hibitory effects of adiponect<strong>in</strong> on steroidogenesis are primarily localized to theca cells and that the response system of theca cells to adiponect<strong>in</strong> (i.e., ADIPOR2) may be regulated dur<strong>in</strong>g follicle growth by LH and IGF-I. P387 Renal abscesses as a complication of ovaryhisterectomy <strong>in</strong> a bitch Vicente, WRR 1 *, Me<strong>de</strong>iros, MG 1 , Pereira, ML 2 , Bürger, C 2 , Voorwald, FA 1 , Motheo, TF 1 , Carvalho, MB 2 , Toniollo, GH 1 1Animal <strong>Reproduction</strong>, Faculty of Agriculture and Veter<strong>in</strong>ary Sciences, São Paulo State University, Brazil; 2 Veter<strong>in</strong>ary Cl<strong>in</strong>ics and Surgery, Faculty of Agriculture and Veter<strong>in</strong>ary Sciences, São Paulo State University, Brazil Rarely, renal abscesses are seen <strong>in</strong> dogs and can occur due to pyelonephritis, nephrolithiasis, and kidney biopsy. The ovaryhisterectomy (OHE) can show numerous complications such as hemorrhage, uter<strong>in</strong>e stump pyometra, pyometra, fistules, urether ligature, hydronephrosis or pyonephrosis, ur<strong>in</strong>ary <strong>in</strong>cont<strong>in</strong>ence, partial colon obstruction and granulomas. To date, there are no reports of OHE complications result<strong>in</strong>g <strong>in</strong> kidney abscesses <strong>in</strong> this specie. A ten year-old Rottweiler female was referred to the Veter<strong>in</strong>ary Teach<strong>in</strong>g Hospital featur<strong>in</strong>g anorexia, oligodipsia and vomit<strong>in</strong>g for three days. The bitch un<strong>de</strong>rwent OHE due to pyometra history three weeks ago. Physical exam<strong>in</strong>ation revealed prostration, mo<strong>de</strong>rated <strong>de</strong>hydration and <strong>in</strong>tense abdom<strong>in</strong>al and lumbar pa<strong>in</strong>. Laboratory f<strong>in</strong>d<strong>in</strong>gs revealed normocytic, normochromic anemia, leukocytosis, mild azotemia, and high levels of alkal<strong>in</strong>e phosphatase. Results of the ur<strong>in</strong>alysis showed isostenuria, hematuria, leukocyturia and bacteriuria. A large round mass appeared on abdom<strong>in</strong>al radiograph as a soft tissue-<strong>de</strong>nsity, caudal to the right si<strong>de</strong> of the liver. Abdom<strong>in</strong>al ultrasound revealed a round structure with multiple fluid-filled areas <strong>in</strong> the same region. Laparotomy was performed for <strong>de</strong>f<strong>in</strong>itive diagnosis. Dur<strong>in</strong>g the procedure, the right kidney was large, round, grey to yellowish, irregular and adhered to the abdom<strong>in</strong>al aorta. Immediately, the animal un<strong>de</strong>rwent nephrectomy. There were cortical abscesses and pelvic recesses thicken<strong>in</strong>g at the sagital section through the kidney. Medical therapy <strong>in</strong>clu<strong>de</strong>d fluidtherapy for ten days, <strong>in</strong> addition to metronidazole (15 mg/kg PO, bid), tramadol (2 mg/kg PO, tid) and ranitid<strong>in</strong>e (2.2 mg/kg PO, bid) for five days, and enrofloxac<strong>in</strong> (5 mg/kg PO, bid) for sixty days. Despite chronic renal failure, the patient was discharged after sixty days from the surgery, and recovered uneventfully. In summary, we hypostatized that the abscesses formation was due to a lesion <strong>in</strong> the capsule of the kidney that was promoted by a complication dur<strong>in</strong>g the OHE procedure itself. P388 Effects of endothel<strong>in</strong>-1 (ET-1) a vasoconstrictor and bradyk<strong>in</strong><strong>in</strong> (BK) a vasodilator on luteal function <strong>in</strong> vitro <strong>in</strong> ewes Weems, Y 1 *; Johnson, D 1 ; Uchima, T 1 ; Raney, A 1 ; Lennon, E 1 ; Bowers, G 1 ; Ran<strong>de</strong>l, R 2 ; Weems, C, 1 1Dept. of HNFAS, University of Hawaii, USA; 2 Agricultural Research and Extension Center, Texas A&M University at Overton, USA PGF 2 α is the uter<strong>in</strong>e luteolys<strong>in</strong> <strong>de</strong>livered locally from the uterus to the adjacent corpus luteum (CL)-conta<strong>in</strong><strong>in</strong><strong>in</strong>g ovary. However, cow CL secretes PGF 2 α before the onset of luteolysis, but it also secretes the PGE at a PGE:PGF 2 α ratio of 1:1 and PGE1 and PGE2 prevents luteolysis (Weems et al. 2006; The Vet J:171:206-228 for review). ET-1 has been reported to mediate PGF2α-<strong>in</strong>duced luteolysis (Milvae, Rev. Reprod. 5:1, 2000). Amounts of mRNA encod<strong>in</strong>g ET-1 convert<strong>in</strong>g enzyme-1 (ECE-1), pre-pro ET-1, and the ET receptors (ETA, ETB) <strong>in</strong>creased <strong>in</strong> bov<strong>in</strong>e luteal tissue from days 1 through day 10 postestrus, amounts on day 17 were similar to day 10, and were not <strong>in</strong>creased by PGF2α on day 10 when CL are responsive to the luteolytic actions of PGF2α, but were <strong>in</strong>creased by exogenous PGF2α on day 17 only when luteolysis was already un<strong>de</strong>rway (Choudhary et al., Domest. Anim. Endocr<strong>in</strong>ol. 27:63, 2004). In addition, Nitric oxi<strong>de</strong> (NO) has been reported to be luteolytic by some <strong>in</strong> cows (Weems et al. 2006; The Vet J:171:206-228 for review). Furthermore, ET-1 and NO donors <strong>in</strong>creased PGE secretion by bov<strong>in</strong>e CL slices <strong>in</strong> vitro when estrus was not synchronized or when estrus was synchronized with PGF2α and did not affect CL PGF2α or progesterone secretion a . In addition, ET-1 or NO donors <strong>in</strong>fused chronically <strong>in</strong>trauter<strong>in</strong>e or <strong>in</strong>to the <strong>in</strong>terstitial tissue of the CL-conta<strong>in</strong><strong>in</strong>g ov<strong>in</strong>e ovary <strong>de</strong>layed luteolysis. Therefore, the objective of this experiment was to <strong>de</strong>term<strong>in</strong>e whether ET-1 affected progesterone, PGE, or PGF2α secretion by ov<strong>in</strong>e CL. Days-12 or 16 ov<strong>in</strong>e CL were collected, weighed, and slices were <strong>in</strong>cubated <strong>in</strong> vitro with Vehicle, ET-1, Bradyk<strong>in</strong><strong>in</strong> (BK-vasodilator), Bradyzi<strong>de</strong> (BZ-BK2 receptor antagonist) or HOE-140 (BK1 receptor antagonist) at 39 C for 1 hour without treatments and for 4 and 8 hours with treatments. Media collected at 4 and 8 hours were analyzed for progesterone, PGE, and PGF2α by RIA. CL weights were analyzed by a One Way ANOVA. Hormone data were analyzed by a 2X5 Factorial Design for ANOVA. CL weights differed (P