Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

More documents

Recommendations

Info

$(Microsoft PowerPoint - Lecci\363n 2_07.ppt)$

16 t h International Congress on Animal Reproduction 154 Poster Abstracts (BHB>1 mmol/l) the average apoptosis index was 23,83±6,44%, it is significantly less than the result of the normoketonaemic (BHB
16 t h International Congress on Animal Reproduction Poster Abstracts 155 acid supplementation can stimulate follicular dynamics and minimize post-partum anestrous. P386 Role of Adiponectin in Regulating Ovarian Theca and Granulosa Cell Function in Cattle Spicer, LJ*; Lagaly, DV; Aad, PY; Grado-Ahuir, JA; Hulsey, LB Animal Science, Oklahoma State University, United States Adiponectin is an adipokine that has been implicated in insulin resistance, a condition associated with polycystic ovarian syndrome in humans, and alters steroid production by rat and pig granulosa cells. Furthermore, adiponectin receptor mRNA has recently been localized within ovarian tissue of rats and chickens, but whether adiponectin can directly affect ovarian theca or granulosa cell function in cattle is unknown. Therefore, experiments were conducted to determine the effects of adiponectin on proliferation, steroidogenesis and gene expression of large-follicle theca and granulosa cells as well as compare adiponectin receptor 2 (ADIPOR2) mRNA abundance in theca and granulosa cells of small and large follicles. Fluorescent real-time quantitative RT-PCR was used to elucidate the effects of adiponectin on gene expression of side-chain cleavage enzyme (CYP11A1) and LH receptor (LHR) in large-follicle theca and granulosa cells, as well as expression of 17-hydroxylase (CYP17A1) in theca cells and aromatase (CYP19A1) in granulosa cells. Adiponectin (3 μg/ml) attenuated insulin-like growth factor-I (IGF-I)- induced LHR, CYP11A1, and CYP17A1 gene expression in theca cells as well as decreased (P < 0.05) LH plus insulin-induced progesterone and androstenedione production by theca cells. In contrast, adiponectin (3 μg/ml) decreased (P < 0.05) LHR mRNA abundance in granulosa cells but did not affect steroidogenic enzyme gene expression in granulosa cells. Theca cells from large (8-22 mm) follicles had fourfold greater (P < 0.05) abundance of ADIPOR2 mRNA than theca cells from small (2-6 mm) follicles. In contrast, granulosa cells from small and large follicles had similar (P > 0.10) ADIPOR2 mRNA abundance and these levels did not significantly differ from those of small-follicle theca cells. To evaluate if hormones regulate ADIPOR2, theca cells from large follicles were incubated in the presence of 0 or 30 ng/ml of IGF-I without or with 30 ng/ml of LH for 24 h. IGF-I decreased (P < 0.05) abundance of ADIPOR2 mRNA by 14% in untreated theca cells but had no effect (P > 0.10) on ADIPOR2 mRNA abundance in LH-treated largefollicle theca cells. In contrast, LH increased (P < 0.05) theca cell ADIPOR2 mRNA abundance by 17% and 27% in the absence and presence of IGF-I, respectively. These results indicate that the inhibitory effects of adiponectin on steroidogenesis are primarily localized to theca cells and that the response system of theca cells to adiponectin (i.e., ADIPOR2) may be regulated during follicle growth by LH and IGF-I. P387 Renal abscesses as a complication of ovaryhisterectomy in a bitch Vicente, WRR 1 *, Medeiros, MG 1 , Pereira, ML 2 , Bürger, C 2 , Voorwald, FA 1 , Motheo, TF 1 , Carvalho, MB 2 , Toniollo, GH 1 1Animal Reproduction, Faculty of Agriculture and Veterinary Sciences, São Paulo State University, Brazil; 2 Veterinary Clinics and Surgery, Faculty of Agriculture and Veterinary Sciences, São Paulo State University, Brazil Rarely, renal abscesses are seen in dogs and can occur due to pyelonephritis, nephrolithiasis, and kidney biopsy. The ovaryhisterectomy (OHE) can show numerous complications such as hemorrhage, uterine stump pyometra, pyometra, fistules, urether ligature, hydronephrosis or pyonephrosis, urinary incontinence, partial colon obstruction and granulomas. To date, there are no reports of OHE complications resulting in kidney abscesses in this specie. A ten year-old Rottweiler female was referred to the Veterinary Teaching Hospital featuring anorexia, oligodipsia and vomiting for three days. The bitch underwent OHE due to pyometra history three weeks ago. Physical examination revealed prostration, moderated dehydration and intense abdominal and lumbar pain. Laboratory findings revealed normocytic, normochromic anemia, leukocytosis, mild azotemia, and high levels of alkaline phosphatase. Results of the urinalysis showed isostenuria, hematuria, leukocyturia and bacteriuria. A large round mass appeared on abdominal radiograph as a soft tissue-density, caudal to the right side of the liver. Abdominal ultrasound revealed a round structure with multiple fluid-filled areas in the same region. Laparotomy was performed for definitive diagnosis. During the procedure, the right kidney was large, round, grey to yellowish, irregular and adhered to the abdominal aorta. Immediately, the animal underwent nephrectomy. There were cortical abscesses and pelvic recesses thickening at the sagital section through the kidney. Medical therapy included fluidtherapy for ten days, in addition to metronidazole (15 mg/kg PO, bid), tramadol (2 mg/kg PO, tid) and ranitidine (2.2 mg/kg PO, bid) for five days, and enrofloxacin (5 mg/kg PO, bid) for sixty days. Despite chronic renal failure, the patient was discharged after sixty days from the surgery, and recovered uneventfully. In summary, we hypostatized that the abscesses formation was due to a lesion in the capsule of the kidney that was promoted by a complication during the OHE procedure itself. P388 Effects of endothelin-1 (ET-1) a vasoconstrictor and bradykinin (BK) a vasodilator on luteal function in vitro in ewes Weems, Y 1 *; Johnson, D 1 ; Uchima, T 1 ; Raney, A 1 ; Lennon, E 1 ; Bowers, G 1 ; Randel, R 2 ; Weems, C, 1 1Dept. of HNFAS, University of Hawaii, USA; 2 Agricultural Research and Extension Center, Texas A&M University at Overton, USA PGF 2 α is the uterine luteolysin delivered locally from the uterus to the adjacent corpus luteum (CL)-containining ovary. However, cow CL secretes PGF 2 α before the onset of luteolysis, but it also secretes the PGE at a PGE:PGF 2 α ratio of 1:1 and PGE1 and PGE2 prevents luteolysis (Weems et al. 2006; The Vet J:171:206-228 for review). ET-1 has been reported to mediate PGF2α-induced luteolysis (Milvae, Rev. Reprod. 5:1, 2000). Amounts of mRNA encoding ET-1 converting enzyme-1 (ECE-1), pre-pro ET-1, and the ET receptors (ETA, ETB) increased in bovine luteal tissue from days 1 through day 10 postestrus, amounts on day 17 were similar to day 10, and were not increased by PGF2α on day 10 when CL are responsive to the luteolytic actions of PGF2α, but were increased by exogenous PGF2α on day 17 only when luteolysis was already underway (Choudhary et al., Domest. Anim. Endocrinol. 27:63, 2004). In addition, Nitric oxide (NO) has been reported to be luteolytic by some in cows (Weems et al. 2006; The Vet J:171:206-228 for review). Furthermore, ET-1 and NO donors increased PGE secretion by bovine CL slices in vitro when estrus was not synchronized or when estrus was synchronized with PGF2α and did not affect CL PGF2α or progesterone secretion a . In addition, ET-1 or NO donors infused chronically intrauterine or into the interstitial tissue of the CL-containing ovine ovary delayed luteolysis. Therefore, the objective of this experiment was to determine whether ET-1 affected progesterone, PGE, or PGF2α secretion by ovine CL. Days-12 or 16 ovine CL were collected, weighed, and slices were incubated in vitro with Vehicle, ET-1, Bradykinin (BK-vasodilator), Bradyzide (BZ-BK2 receptor antagonist) or HOE-140 (BK1 receptor antagonist) at 39 C for 1 hour without treatments and for 4 and 8 hours with treatments. Media collected at 4 and 8 hours were analyzed for progesterone, PGE, and PGF2α by RIA. CL weights were analyzed by a One Way ANOVA. Hormone data were analyzed by a 2X5 Factorial Design for ANOVA. CL weights differed (P
Page 1 and 2:
REPRODUCTION IN DOMESTIC ANIMALS 43
Page 3 and 4:
16th International Congress on Anim
Page 5 and 6:
16 t h International Congress on An
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106: 16 t h International Congress on An
Page 155: 16 t h International Congress on An
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
show all

Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

Create successful ePaper yourself

Delete template?

Save as template?