Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
138 Poster Abstracts
In this study, 50 male Wister rats, each weighing 282±110g were
used. These animals were divided into five groups of tens: the control
group receiving normal food, the sham group receiving solvent
(distilled water), and three experimental groups receiving 0.05, 0.1,
and 0.2 g/kg extract of spathe respectively. The dosages of distilled
water and extracts were injected intra peritoneally for 14 days. Eight
hours after receiving last doses blood samples were taken and serum
concentration of lutein hormone (LH) follico stimulation hormone
(FSH) and testosterone were measured by RIA method.The results
were evaluated by SPSS and Tukey test.
Statistical analysis of the results indicates that concentration of
testosterone decreases significantly in experimental groups receiving
0.05 and 0.1 g/Kg extract, while serum concentration of LH and FSH
did not show any significant difference (π≤0.05) among various
groups.
According to the results alcoholic extract of phoenix dactylifera
spathe reduces serum testosterone and protein synthesis. In addition,
the presence of phytosterols in the extract which inhibit 5-a-reductase
and aromatase enzymes activities may diminish tissues sensitivity to
androgens. Finally, the reduction of sperms in somniferous tubules
may due to the estrogenic activities of phytosterols and coumarin.
P335
Synchronization of oestrus and ovulation in mice by
administration of progesterone and prostaglandin
analogues
Pallares, P 1 * and Gonzalez-Bulnes, A 2
1Fundacion CNIC. Madrid, Spain; 2 Dpto. Reproduccion Animal. INIA. Madrid,
Spain
Introduction Management of the oestrous cycle in the mouse is
scarce. Usual methods for induction of behavioural oestrus and
ovulation are “male effect”, by the introduction of a male, fertile or
vasectomized and “Whitten effect”, by the exposition to male
pheromones; however, the degree of oestrus synchronization reached
with these methods is very limited. Thus, we aimed to design and
establish a protocol for induction and synchronization of oestrus and
ovulation in mice, based in exogenous hormones.
Material and methods In four consecutive experiments, a total of 72
adult mice in breeding age were used to evaluate effectiveness of the
use, alone and combined, of exogenous progesterone and
prostaglandin analogues. Different strains (37 BALB/c, 10 C57BL/6
and 25 CD1) were treated for testing breed effects. All the animals
were maintained at the facilities of the CNIC Animal Laboratory Unit
in Madrid, Spain.
Results The higher synchronization degree and the higher fertility
rates were obtained by the use of a protocol consisting of two i.p.
doses of 0.5μg of cloprostenol, three days apart, plus a single s.c. dose
of 3μg of progesterone coincidentally with the first injection of
cloprostenol. Within main advantages of the new method, we have to
highlight the short time elapsed for appearance, and the high degree of
synchronization, of oestrus and ovulations (almost 100% of the
animals responding to the treatment in 48h; 78.4% with fertile mates
at 24h), plus the high fertility rate obtained after a programmed
mating (100%). The response was very repeatable between replicates,
which may be related to a high synchronization of the ovarian stage at
induction of luteolysis and introduction of the male. There were not
found significant differences between strains, except the expected
effect on prolificacy (P