Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
138 Poster Abstracts<br />
In this study, 50 male Wister rats, each weigh<strong>in</strong>g 282±110g were<br />
used. These animals were divi<strong>de</strong>d <strong>in</strong>to five groups of tens: the control<br />
group receiv<strong>in</strong>g normal food, the sham group receiv<strong>in</strong>g solvent<br />
(distilled water), and three experimental groups receiv<strong>in</strong>g 0.05, 0.1,<br />
and 0.2 g/kg extract of spathe respectively. The dosages of distilled<br />
water and extracts were <strong>in</strong>jected <strong>in</strong>tra peritoneally for 14 days. Eight<br />
hours after receiv<strong>in</strong>g last doses blood samples were taken and serum<br />
concentration of lute<strong>in</strong> hormone (LH) follico stimulation hormone<br />
(FSH) and testosterone were measured by RIA method.The results<br />
were evaluated by SPSS and Tukey test.<br />
Statistical analysis of the results <strong>in</strong>dicates that concentration of<br />
testosterone <strong>de</strong>creases significantly <strong>in</strong> experimental groups receiv<strong>in</strong>g<br />
0.05 and 0.1 g/Kg extract, while serum concentration of LH and FSH<br />
did not show any significant difference (π≤0.05) among various<br />
groups.<br />
Accord<strong>in</strong>g to the results alcoholic extract of phoenix dactylifera<br />
spathe reduces serum testosterone and prote<strong>in</strong> synthesis. In addition,<br />
the presence of phytosterols <strong>in</strong> the extract which <strong>in</strong>hibit 5-a-reductase<br />
and aromatase enzymes activities may dim<strong>in</strong>ish tissues sensitivity to<br />
androgens. F<strong>in</strong>ally, the reduction of sperms <strong>in</strong> somniferous tubules<br />
may due to the estrogenic activities of phytosterols and coumar<strong>in</strong>.<br />
P335<br />
Synchronization of oestrus and ovulation <strong>in</strong> mice by<br />
adm<strong>in</strong>istration of progesterone and prostagland<strong>in</strong><br />
analogues<br />
Pallares, P 1 * and Gonzalez-Bulnes, A 2<br />
1Fundacion CNIC. Madrid, Spa<strong>in</strong>; 2 Dpto. Reproduccion Animal. INIA. Madrid,<br />
Spa<strong>in</strong><br />
Introduction Management of the oestrous cycle <strong>in</strong> the mouse is<br />
scarce. Usual methods for <strong>in</strong>duction of behavioural oestrus and<br />
ovulation are “male effect”, by the <strong>in</strong>troduction of a male, fertile or<br />
vasectomized and “Whitten effect”, by the exposition to male<br />
pheromones; however, the <strong>de</strong>gree of oestrus synchronization reached<br />
with these methods is very limited. Thus, we aimed to <strong>de</strong>sign and<br />
establish a protocol for <strong>in</strong>duction and synchronization of oestrus and<br />
ovulation <strong>in</strong> mice, based <strong>in</strong> exogenous hormones.<br />
Material and methods In four consecutive experiments, a total of 72<br />
adult mice <strong>in</strong> breed<strong>in</strong>g age were used to evaluate effectiveness of the<br />
use, alone and comb<strong>in</strong>ed, of exogenous progesterone and<br />
prostagland<strong>in</strong> analogues. Different stra<strong>in</strong>s (37 BALB/c, 10 C57BL/6<br />
and 25 CD1) were treated for test<strong>in</strong>g breed effects. All the animals<br />
were ma<strong>in</strong>ta<strong>in</strong>ed at the facilities of the CNIC Animal Laboratory Unit<br />
<strong>in</strong> Madrid, Spa<strong>in</strong>.<br />
Results The higher synchronization <strong>de</strong>gree and the higher fertility<br />
rates were obta<strong>in</strong>ed by the use of a protocol consist<strong>in</strong>g of two i.p.<br />
doses of 0.5μg of cloprostenol, three days apart, plus a s<strong>in</strong>gle s.c. dose<br />
of 3μg of progesterone co<strong>in</strong>ci<strong>de</strong>ntally with the first <strong>in</strong>jection of<br />
cloprostenol. With<strong>in</strong> ma<strong>in</strong> advantages of the new method, we have to<br />
highlight the short time elapsed for appearance, and the high <strong>de</strong>gree of<br />
synchronization, of oestrus and ovulations (almost 100% of the<br />
animals respond<strong>in</strong>g to the treatment <strong>in</strong> 48h; 78.4% with fertile mates<br />
at 24h), plus the high fertility rate obta<strong>in</strong>ed after a programmed<br />
mat<strong>in</strong>g (100%). The response was very repeatable between replicates,<br />
which may be related to a high synchronization of the ovarian stage at<br />
<strong>in</strong>duction of luteolysis and <strong>in</strong>troduction of the male. There were not<br />
found significant differences between stra<strong>in</strong>s, except the expected<br />
effect on prolificacy (P