Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
136 Poster Abstracts
(BSA) as a model for establishing criteria for human IVF and GIFT
procedures.
Design Optimum concentrations of ECS and BFF in Ham's F-10 were
established by measuring blastocyst development of in vivo fertilized
zygotes from a mouse strain.
Animals eight-week-old, superovulated mice.
Result(s) In vivo-derived embryos were cultured in Ham's F-10, to
which one of the following groups: [1] BSA (4 mg/ml), [2] different
concentrations (10 and 20%) of ECS [3] different concentrations (10
and 20%) of FF, added two-cell stage. The proportion of embryos
developing was affected by the type of protein and concentration.
Reduced cleaved rates of embryo development were observed in
Ham's F-10 supplemented with 10% BFF and 10% ECS. The rates of
development of balstocystes in vitro were suppressed when the
embryos were cultured with 10% BFF or 10% ECS as compared with
BSA. Ham's F-10 supplemented with 20% ECS, 20% BFF and BSA
also supported the development of in vitro-derive embryos (P