Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 129<br />
Gona<strong>de</strong>ctomy not only affects hormonal homeostasis but also alters<br />
the turnover of different components of the extracellular matrix <strong>in</strong><br />
urogenital tissues. Collagen is an important component of the blad<strong>de</strong>r<br />
and urethral walls and thus crucial for the mechanical properties of<br />
normal lower ur<strong>in</strong>ary tract (LUT) functions. Collagen synthesis<br />
appears to be modified by the hormonal environment, specifically<br />
oestrogen and gonadotroph<strong>in</strong> levels, and receptors for these hormones<br />
are expressed <strong>in</strong> the LUT. Interest<strong>in</strong>gly, the expression of these<br />
receptors varies between <strong>in</strong>tact and gona<strong>de</strong>ctomised dogs. The<br />
objective of the present study was to <strong>de</strong>term<strong>in</strong>e whether there is any<br />
difference <strong>in</strong> proportions of collagen and muscle tissues <strong>in</strong> the LUT of<br />
<strong>in</strong>tact and gona<strong>de</strong>ctomised male and female dogs.<br />
Materials and Methods All the animals used <strong>in</strong> this study were<br />
cl<strong>in</strong>ically healthy and <strong>in</strong>clu<strong>de</strong>d 10 <strong>in</strong>tact dogs (5 males, 5 females) and<br />
10 gona<strong>de</strong>ctomised dogs (4 males and 6 females). Four regions of<br />
LUT <strong>in</strong>clud<strong>in</strong>g body and neck of the blad<strong>de</strong>r as well as proximal and<br />
distal urethra were collected. The proportion of collagen and muscle<br />
were <strong>de</strong>term<strong>in</strong>ed by sta<strong>in</strong><strong>in</strong>g the tissue sections with Masson’s<br />
trichrome, which highlights collagen as blue and muscle as red.<br />
Colour image analysis was performed on digitized computer data<br />
us<strong>in</strong>g the Leica Q W<strong>in</strong> Version 3 Quips Programm<strong>in</strong>g Software. The<br />
proportion of blue and red sta<strong>in</strong><strong>in</strong>g with<strong>in</strong> each image was calculated<br />
to <strong>de</strong>term<strong>in</strong>e relative proportions of collagen and muscle <strong>in</strong> each<br />
sample. Data were statistically analyzed us<strong>in</strong>g Mixed Mo<strong>de</strong>l<br />
ANOVA.<br />
Results The proportion of collagen and muscle tissue differed with<br />
the reproductive status (<strong>in</strong>tact and gona<strong>de</strong>ctomised) and gen<strong>de</strong>r.<br />
Gona<strong>de</strong>ctomised dogs had a higher (P