Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
124 Poster Abstracts<br />
similar to or even below basel<strong>in</strong>e values. The collected data suggest a<br />
significant and so far not recognized <strong>in</strong>fluence of spermatozoa on the<br />
regulation of the uter<strong>in</strong>e immune responses after <strong>in</strong>sem<strong>in</strong>ation.<br />
P291<br />
Effect of dietary supplementation with salmon oil on<br />
cryopreservation of boar semen<br />
Amorim, LS 1, Torres, CAA 2 *, Amorim, EAM 2 , Graham, J 2<br />
1Department of Biomedical Sciences, Colorado State University, Fort Coll<strong>in</strong>s,<br />
CO, United States; 2 Animal Science Department, Fe<strong>de</strong>ral University of<br />
Viçosa, M<strong>in</strong>as Gerais, Brazil<br />
Cryopreservation of boar semen is not common, as the damage caused<br />
to the cells is extensive. The fatty acid composition of boar<br />
spermatozoa conta<strong>in</strong> some docosapentaenoic acid (DPA) and<br />
docosahexaenoic acid (DHA). The <strong>in</strong>crease <strong>in</strong> the freezability of boar<br />
spermatozoa by enhanc<strong>in</strong>g the DHA content of the plasma membranes<br />
via changes <strong>in</strong> the lipid content of the feed is consi<strong>de</strong>red. The<br />
objective was to f<strong>in</strong>d out whether DHA, given as salmon oil<br />
supplementation, may have a beneficial effect on cryopreservation of<br />
boar semen. Twenty-four boars Dalboard 85, 1–2 years old, were<br />
distributed <strong>in</strong> a completely randomized factorial <strong>de</strong>sign (2×3) with<br />
two oil sources (soybean and salmon) and three levels of antioxidant<br />
(150, 300, and 450 mg of vitam<strong>in</strong> E/kg). The diets consisted of a basal<br />
diet that was supplemented with 35g soybean or salmon oil (SO) per<br />
kg diet. Dur<strong>in</strong>g a period of 10 weeks of feed<strong>in</strong>g the diets, one<br />
ejaculate from each boar was collected per week. An aliquot of the<br />
sperm rich fraction was diluted 1:1 (v:v) <strong>in</strong> BTS and used for<br />
assessment of fresh semen quality and sperm lipid analysis. Semen<br />
was diluted with BTS at 30 o C and after kepted at 24 o C for 1 h, and<br />
then, centrifuged with centrifugation diluent (CD) and rediluted with a<br />
freez<strong>in</strong>g exten<strong>de</strong>r (50 ml of 11% lactose <strong>in</strong> distilled water + 20 ml of<br />
egg yolk, + 25 ml of CD, 1.5 ml Equex, 6 ml of 85% glycerol) to a<br />
f<strong>in</strong>al concentration of 500x106 cells/ml and filled French straws (0.5<br />
ml; M<strong>in</strong>itub, Brazil) and stored for 1 h at 5 oC. After, samples were<br />
frozen 5 cm above liquid nitrogen. Thaw<strong>in</strong>g was <strong>in</strong> a noncirculat<strong>in</strong>g<br />
water-bath 37 o C for 20s. For <strong>de</strong>term<strong>in</strong><strong>in</strong>g the fatty acid composition<br />
of the spermatozoa, a sample of approximately 15 ml was taken from<br />
each ejaculate shortly after collection and centrifuged for 20 m<strong>in</strong> at<br />
1000 x g. The rema<strong>in</strong><strong>in</strong>g semen was frozen until analysed. Sperm<br />
motility, morphology and lipid composition were assessed <strong>in</strong> fresh<br />
and frozen–thawed samples. The DHA <strong>in</strong>creased <strong>in</strong> the SO-group<br />
from 23.3 to 46.7% and the DPA <strong>de</strong>creased from 11.5 to 5.2%<br />
(P