Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 119<br />
P276<br />
Expression of <strong>in</strong>tegr<strong>in</strong>s, transmembrane prote<strong>in</strong> CD9,<br />
leukocyte adhesion molecule CD18, and zonaglycoprote<strong>in</strong>s<br />
<strong>in</strong> porc<strong>in</strong>e oocytes isolated from<br />
multiparous sows and prepuberal gilts<br />
Kempisty, B 1 *, Antosik, P 2 , Bukowska, D 2 , Jackowska, M 1 , Lianeri, M 1 ,<br />
Jaśkowski, JM 2 , Jagodziński, PP 1<br />
1Department of Biochemistry and Molecular Biology, University of Medical<br />
Sciences, Poznań, Poland; 2 Department of Agricultural Veter<strong>in</strong>ary, University<br />
of Agriculture, Poznań, Poland<br />
The <strong>de</strong>velopmental and meiotic competence of oocytes isolated from<br />
age different female donors is well <strong>de</strong>scribed. However, little is yet<br />
known about the fertilization potential of these oocytes. Therefore, the<br />
aim of this study was to <strong>de</strong>term<strong>in</strong>e the differences <strong>in</strong> the expression<br />
pattern of sperm-oocyte <strong>in</strong>teraction molecules <strong>in</strong> oocytes produced by<br />
multiparous sows and prepuberal gilts.<br />
Us<strong>in</strong>g reverse transcription (RT-PCR) and real-time quantitative PCR<br />
(QR-PCR) analysis we compared the expression levels of <strong>in</strong>tegr<strong>in</strong>s<br />
(αL, αM, β1, β6), leukocyte adhesion molecule CD18, transmembrane<br />
prote<strong>in</strong> CD9, and porc<strong>in</strong>e zona pellucida prote<strong>in</strong>s pZPs (pZP1, pZP2,<br />
pZP3 and pZP3α) <strong>in</strong> oocytes isolated from multiparous sows and<br />
prepuberal gilts.<br />
We found statistically significant <strong>in</strong>creased expression of all <strong>in</strong>tegr<strong>in</strong>s,<br />
CD9, pZP2, and pZP3 mRNA <strong>in</strong> oocytes collected from prepuberal<br />
gilts as compared to multiparous sows (P