Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 118 Poster Abstracts P273 Modifications of prostaglandin synthesis enzymes gene expression in the porcine oviduct after intrauterine infusion of seminal plasma Kaczmarek, MM*; Krawczynski, K; Kiewisz, J; Ziecik, AJ Department of Hormonal Action Mechanisms, Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland Introduction Recent studies have indicated that introduction of semen/seminal plasma (SP) into the female reproductive tract orchestrates striking molecular and cellular changes that facilitate embryo development, conception and pregnancy. These changes were also observed in the expression of enzymes of prostaglandin (PG) synthesis pathway, however until now only seminal-induced expression of PGHS-2 (prostaglandin-endoperoxide synthase 2) mRNA in the porcine endometrium has been showed. Since the oviduct plays a decisive role in reproduction providing a beneficial environment for gamete maturation, fertilization and the early embryonic development we have investigated whether intrauterine infusion of SP can modulate PG synthesis in the porcine oviduct through regulation of prostaglandin synthesis enzymes gene expression. Methods Fifty six synchronized crossbred gilts received 100 ml intrauterine infusion of either SP or phosphate buffered saline (PBS; control). Oviducts were collected 1, 3, 5 and 10 Day(s) after SP or PBS-treatment. Expression of PGHS-2, PGF synthase (PGFS), PGE synthase (mPGES-1), PG 9-ketoreductase (9-KR) and PGI synthase (PGIS) mRNA in oviducts was assessed by the real time PCR. Results Among tested enzymes only PGFS and 9-KR mRNA were significantly lower (4.6- and 2.1-fold, respectively) in oviducts 24 h after SP infusion into the uterine horns, when compared to PBStreated animals (P
16 t h International Congress on Animal Reproduction Poster Abstracts 119 P276 Expression of integrins, transmembrane protein CD9, leukocyte adhesion molecule CD18, and zonaglycoproteins in porcine oocytes isolated from multiparous sows and prepuberal gilts Kempisty, B 1 *, Antosik, P 2 , Bukowska, D 2 , Jackowska, M 1 , Lianeri, M 1 , Jaśkowski, JM 2 , Jagodziński, PP 1 1Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland; 2 Department of Agricultural Veterinary, University of Agriculture, Poznań, Poland The developmental and meiotic competence of oocytes isolated from age different female donors is well described. However, little is yet known about the fertilization potential of these oocytes. Therefore, the aim of this study was to determine the differences in the expression pattern of sperm-oocyte interaction molecules in oocytes produced by multiparous sows and prepuberal gilts. Using reverse transcription (RT-PCR) and real-time quantitative PCR (QR-PCR) analysis we compared the expression levels of integrins (αL, αM, β1, β6), leukocyte adhesion molecule CD18, transmembrane protein CD9, and porcine zona pellucida proteins pZPs (pZP1, pZP2, pZP3 and pZP3α) in oocytes isolated from multiparous sows and prepuberal gilts. We found statistically significant increased expression of all integrins, CD9, pZP2, and pZP3 mRNA in oocytes collected from prepuberal gilts as compared to multiparous sows (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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