Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
118 Poster Abstracts
P273
Modifications of prostaglandin synthesis enzymes gene
expression in the porcine oviduct after intrauterine
infusion of seminal plasma
Kaczmarek, MM*; Krawczynski, K; Kiewisz, J; Ziecik, AJ
Department of Hormonal Action Mechanisms, Division of Reproductive
Endocrinology and Pathophysiology, Institute of Animal Reproduction and
Food Research, Polish Academy of Sciences, Olsztyn, Poland
Introduction Recent studies have indicated that introduction of
semen/seminal plasma (SP) into the female reproductive tract
orchestrates striking molecular and cellular changes that facilitate
embryo development, conception and pregnancy. These changes were
also observed in the expression of enzymes of prostaglandin (PG)
synthesis pathway, however until now only seminal-induced
expression of PGHS-2 (prostaglandin-endoperoxide synthase 2)
mRNA in the porcine endometrium has been showed. Since the
oviduct plays a decisive role in reproduction providing a beneficial
environment for gamete maturation, fertilization and the early
embryonic development we have investigated whether intrauterine
infusion of SP can modulate PG synthesis in the porcine oviduct
through regulation of prostaglandin synthesis enzymes gene
expression.
Methods Fifty six synchronized crossbred gilts received 100 ml
intrauterine infusion of either SP or phosphate buffered saline (PBS;
control). Oviducts were collected 1, 3, 5 and 10 Day(s) after SP or
PBS-treatment. Expression of PGHS-2, PGF synthase (PGFS), PGE
synthase (mPGES-1), PG 9-ketoreductase (9-KR) and PGI synthase
(PGIS) mRNA in oviducts was assessed by the real time PCR.
Results Among tested enzymes only PGFS and 9-KR mRNA were
significantly lower (4.6- and 2.1-fold, respectively) in oviducts 24 h
after SP infusion into the uterine horns, when compared to PBStreated
animals (P