Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
118 Poster Abstracts<br />
P273<br />
Modifications of prostagland<strong>in</strong> synthesis enzymes gene<br />
expression <strong>in</strong> the porc<strong>in</strong>e oviduct after <strong>in</strong>trauter<strong>in</strong>e<br />
<strong>in</strong>fusion of sem<strong>in</strong>al plasma<br />
Kaczmarek, MM*; Krawczynski, K; Kiewisz, J; Ziecik, AJ<br />
Department of Hormonal Action Mechanisms, Division of Reproductive<br />
Endocr<strong>in</strong>ology and Pathophysiology, Institute of Animal <strong>Reproduction</strong> and<br />
Food Research, Polish Aca<strong>de</strong>my of Sciences, Olsztyn, Poland<br />
Introduction Recent studies have <strong>in</strong>dicated that <strong>in</strong>troduction of<br />
semen/sem<strong>in</strong>al plasma (SP) <strong>in</strong>to the female reproductive tract<br />
orchestrates strik<strong>in</strong>g molecular and cellular changes that facilitate<br />
embryo <strong>de</strong>velopment, conception and pregnancy. These changes were<br />
also observed <strong>in</strong> the expression of enzymes of prostagland<strong>in</strong> (PG)<br />
synthesis pathway, however until now only sem<strong>in</strong>al-<strong>in</strong>duced<br />
expression of PGHS-2 (prostagland<strong>in</strong>-endoperoxi<strong>de</strong> synthase 2)<br />
mRNA <strong>in</strong> the porc<strong>in</strong>e endometrium has been showed. S<strong>in</strong>ce the<br />
oviduct plays a <strong>de</strong>cisive role <strong>in</strong> reproduction provid<strong>in</strong>g a beneficial<br />
environment for gamete maturation, fertilization and the early<br />
embryonic <strong>de</strong>velopment we have <strong>in</strong>vestigated whether <strong>in</strong>trauter<strong>in</strong>e<br />
<strong>in</strong>fusion of SP can modulate PG synthesis <strong>in</strong> the porc<strong>in</strong>e oviduct<br />
through regulation of prostagland<strong>in</strong> synthesis enzymes gene<br />
expression.<br />
Methods Fifty six synchronized crossbred gilts received 100 ml<br />
<strong>in</strong>trauter<strong>in</strong>e <strong>in</strong>fusion of either SP or phosphate buffered sal<strong>in</strong>e (PBS;<br />
control). Oviducts were collected 1, 3, 5 and 10 Day(s) after SP or<br />
PBS-treatment. Expression of PGHS-2, PGF synthase (PGFS), PGE<br />
synthase (mPGES-1), PG 9-ketoreductase (9-KR) and PGI synthase<br />
(PGIS) mRNA <strong>in</strong> oviducts was assessed by the real time PCR.<br />
Results Among tested enzymes only PGFS and 9-KR mRNA were<br />
significantly lower (4.6- and 2.1-fold, respectively) <strong>in</strong> oviducts 24 h<br />
after SP <strong>in</strong>fusion <strong>in</strong>to the uter<strong>in</strong>e horns, when compared to PBStreated<br />
animals (P