Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 117<br />
embryos cultured without PES (control, 100%; TI=1.0). In<br />
conclusion, our results showed a significant <strong>de</strong>crease of lipid content<br />
<strong>in</strong> lipid droplets of porc<strong>in</strong>e embryos after <strong>in</strong> vitro culture <strong>in</strong> PESconta<strong>in</strong><strong>in</strong>g<br />
medium. The f<strong>in</strong>d<strong>in</strong>gs also suggest that PES reduced<br />
accumulation of lipids <strong>in</strong> cultured pig embryos.<br />
P270<br />
The us<strong>in</strong>g of the DSI telemetry implants <strong>in</strong> the<br />
reproductive tract EMG record<strong>in</strong>g <strong>in</strong> the sows <strong>in</strong> relation<br />
to LH, P4,E2<br />
Gajewski, Z 1 *, Pawliński, B 1 , Ziecik, AJ 2 , Zabielski, R 3<br />
1Animal <strong>Reproduction</strong>, Warsaw University of Life Sciences, Faculty of<br />
Veter<strong>in</strong>ary Medic<strong>in</strong>e, Poland; 2 Institute of Animal Reprod. and Food Res.,<br />
Poland; 3 Physiology, Warsaw University of Life Sciences, Faculty of<br />
Veter<strong>in</strong>ary Medic<strong>in</strong>e, Poland<br />
Introduction The method of radiotelemetry system allows<br />
transformation of biological signals from animal body <strong>in</strong>to the radio<br />
waves. Knowledge on oviduct electrical and motor activity is limited<br />
though crucial for un<strong>de</strong>rstand<strong>in</strong>g the physiology and pathophysiology<br />
of the reproductive system. The objective of the present studies was to<br />
adapt implantable telemetry technique for uterus and oviducts EMG<br />
studies and exam<strong>in</strong>e relationship between LH, E2, P4 level and EMG<br />
activity of oviduct and uter<strong>in</strong>e horn <strong>in</strong> sows with <strong>in</strong>duced estrus<br />
(PMSG/HCG).<br />
Materials and Methods For exam<strong>in</strong>ations we used commercial<br />
implants (DSI, USA) and silver bipolar electro<strong>de</strong>s. 11 nonpregnant<br />
sows were surgically fitted with TL10M3-D70-EEE (DSI, USA)<br />
implants positioned between the abdom<strong>in</strong>al muscles, and 3 silicone<br />
electro<strong>de</strong>s sutured on the left or right oviduct (bulb and mid part) and<br />
the correspond<strong>in</strong>g uterus horn. Three signal channels was filtered<br />
(high cut-off 50 Hz, low-cut 10 Hz) and amplified (BioAmp,<br />
ADInstruments, Australia). A four-channel PowerLab/4e unit and PC<br />
computer with Chart v.4.1 (ADInstruments) software were used to<br />
record, display and analyze the data. Blood samples were taken from<br />
the jugular ve<strong>in</strong>, stored at -20oC, until LH, P4 and E2 RIA analyses.<br />
Results Registration was performed on the end of follicular phase of<br />
estrous cycle i.e. before beg<strong>in</strong>n<strong>in</strong>g of LH surge. A rise of progesterone<br />
secretion (1-4 ng/ml) was found 36-40 hrs after preovulatory LH<br />
surge. The E2 <strong>de</strong>crease after the LH surge from 150-180 pg/ml to 75-<br />
85 pg/ml. Mean burst duration of ampulla contractions was 30.5 ± 2.4<br />
s and the total duration of electrical activity tested 736.4 s. The same<br />
parameters recor<strong>de</strong>d from the uter<strong>in</strong>e horn were 27.0±0.9 and 520.0 s,<br />
respectively dur<strong>in</strong>g 30 m<strong>in</strong> of observation. The frequency of burst was<br />
27 <strong>in</strong> the ampulla of oviduct and 21 <strong>in</strong> the uter<strong>in</strong>e horn for 30 m<strong>in</strong><br />
period of record<strong>in</strong>g. The mean amplitu<strong>de</strong> (mV) of spikes <strong>in</strong>si<strong>de</strong> burst<br />
was significantly higher <strong>in</strong> uter<strong>in</strong>e horn than <strong>in</strong> ampulla of oviduct<br />
(0.86±0.04 vs 0.32±0.01, respectively; p