Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
Poster Abstracts 117
embryos cultured without PES (control, 100%; TI=1.0). In
conclusion, our results showed a significant decrease of lipid content
in lipid droplets of porcine embryos after in vitro culture in PEScontaining
medium. The findings also suggest that PES reduced
accumulation of lipids in cultured pig embryos.
P270
The using of the DSI telemetry implants in the
reproductive tract EMG recording in the sows in relation
to LH, P4,E2
Gajewski, Z 1 *, Pawliński, B 1 , Ziecik, AJ 2 , Zabielski, R 3
1Animal Reproduction, Warsaw University of Life Sciences, Faculty of
Veterinary Medicine, Poland; 2 Institute of Animal Reprod. and Food Res.,
Poland; 3 Physiology, Warsaw University of Life Sciences, Faculty of
Veterinary Medicine, Poland
Introduction The method of radiotelemetry system allows
transformation of biological signals from animal body into the radio
waves. Knowledge on oviduct electrical and motor activity is limited
though crucial for understanding the physiology and pathophysiology
of the reproductive system. The objective of the present studies was to
adapt implantable telemetry technique for uterus and oviducts EMG
studies and examine relationship between LH, E2, P4 level and EMG
activity of oviduct and uterine horn in sows with induced estrus
(PMSG/HCG).
Materials and Methods For examinations we used commercial
implants (DSI, USA) and silver bipolar electrodes. 11 nonpregnant
sows were surgically fitted with TL10M3-D70-EEE (DSI, USA)
implants positioned between the abdominal muscles, and 3 silicone
electrodes sutured on the left or right oviduct (bulb and mid part) and
the corresponding uterus horn. Three signal channels was filtered
(high cut-off 50 Hz, low-cut 10 Hz) and amplified (BioAmp,
ADInstruments, Australia). A four-channel PowerLab/4e unit and PC
computer with Chart v.4.1 (ADInstruments) software were used to
record, display and analyze the data. Blood samples were taken from
the jugular vein, stored at -20oC, until LH, P4 and E2 RIA analyses.
Results Registration was performed on the end of follicular phase of
estrous cycle i.e. before beginning of LH surge. A rise of progesterone
secretion (1-4 ng/ml) was found 36-40 hrs after preovulatory LH
surge. The E2 decrease after the LH surge from 150-180 pg/ml to 75-
85 pg/ml. Mean burst duration of ampulla contractions was 30.5 ± 2.4
s and the total duration of electrical activity tested 736.4 s. The same
parameters recorded from the uterine horn were 27.0±0.9 and 520.0 s,
respectively during 30 min of observation. The frequency of burst was
27 in the ampulla of oviduct and 21 in the uterine horn for 30 min
period of recording. The mean amplitude (mV) of spikes inside burst
was significantly higher in uterine horn than in ampulla of oviduct
(0.86±0.04 vs 0.32±0.01, respectively; p