Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 104 Poster Abstracts area and disturbed uterine hemodynamics at the mesometrial attachment. P229 Effect of protein source in stallion semen diluent on the motility, acrosome integrity and morphology of sperm Gibb, Z 1 *, Morris, L 2 , Grupen, C 1 , Evans, G 1 , Maxwell, C 1 1Faculty of Veterinary Science, The University of Sydney, Sydney, Australia; 2EquiBreed Ltd., Cambridge, New Zealand Introduction Most stallion semen diluents utilise skim milk to provide protective proteins for sperm. However, milk based diluents impede sperm motility assessments and reduce the effectiveness of staining procedures used to process sperm for flow cytometric sorting. In addition, the variable protein levels and the presence of unidentified toxic products in milk may vary results. Therefore, a more precisely defined diluent protein composition is needed. The aim of this study was to ascertain the optimal source of protein in a traditional stallion semen diluent, Kenney’s Modified Tyrode’s 1 (KMT) Medium, for handling and processing stallion sperm prior to flow cytometric sex-sorting. Methods and Materials Two ejaculates were collected from each of three Standardbred stallions. Each ejaculate was diluted in traditional KMT that contained skim milk or KMT without skim milk and supplemented with 0.25mg per ml of either bovine serum albumin (BSA), β-lactoglobulin, glycine or fetal bovine serum (FBS). Diluted semen samples were incubated at 34ºC or 5ºC. Subjective motility, acrosome integrity and morphology were assessed over a 24 h period. Results and Discussion After incubation for 3 h at 34ºC, there was no significant difference between the diluents for total motility, progressive motility or acrosome status of spermatozoa. After incubation for 24 h at 34ºC in KMT, there was a lower incidence of sperm tail abnormalities compared with the other diluents (9.3 vs. 23.2 – 24.5 %; p
16 t h International Congress on Animal Reproduction Poster Abstracts 105 P232 Fertility of Thoroughbred stallions with special respect to their use as dual-hemisphere (shuttle) stallions Aurich, C 1 , Höhndorf, U 2 * 1Dept. for Animal Breeding and Reproduction, Centre for Artificial Insemination and Embryo Transfer, Austria; 2 Veterinaermedizinische Universitaet Wien Thoroughbred stallions with a high genetic potential are often used for breeding on the northern (NH) and southern hemisphere (SH) in the consecutive breeding seasons of the same year. These stallions are called shuttle stallions. It was the aim of this study to compare fertility data of shuttle stallions to data of stallions that were just used for one breeding season, i.e. on one hemisphere. Data from the breed registries of Argentina, Australia, Great Britain/Ireland, New Zealand and the USA on number of covered mares per stallion, number of live foals per stallion and the rate of live foals per stallion and season were statistically compared. Data from a total of 6686 stallions (year 2005) were included, 144 of them were used for breeding in at least one country of the NH and the SH in that year and thus were defined as shuttle stallions. In the shuttle stallions, the number of covered mares (62.4±3.1), live foals (42.7±2.2) and live foal rate (69.5±1.3%) per season (average of the two breeding seasons in 2005) was significantly (p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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