Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
104 Poster Abstracts<br />
area and disturbed uter<strong>in</strong>e hemodynamics at the mesometrial<br />
attachment.<br />
P229<br />
Effect of prote<strong>in</strong> source <strong>in</strong> stallion semen diluent on the<br />
motility, acrosome <strong>in</strong>tegrity and morphology of sperm<br />
Gibb, Z 1 *, Morris, L 2 , Grupen, C 1 , Evans, G 1 , Maxwell, C 1<br />
1Faculty of Veter<strong>in</strong>ary Science, The University of Sydney, Sydney, Australia;<br />
2EquiBreed Ltd., Cambridge, New Zealand<br />
Introduction Most stallion semen diluents utilise skim milk to<br />
provi<strong>de</strong> protective prote<strong>in</strong>s for sperm. However, milk based diluents<br />
impe<strong>de</strong> sperm motility assessments and reduce the effectiveness of<br />
sta<strong>in</strong><strong>in</strong>g procedures used to process sperm for flow cytometric sort<strong>in</strong>g.<br />
In addition, the variable prote<strong>in</strong> levels and the presence of<br />
uni<strong>de</strong>ntified toxic products <strong>in</strong> milk may vary results. Therefore, a<br />
more precisely <strong>de</strong>f<strong>in</strong>ed diluent prote<strong>in</strong> composition is nee<strong>de</strong>d. The<br />
aim of this study was to ascerta<strong>in</strong> the optimal source of prote<strong>in</strong> <strong>in</strong> a<br />
traditional stallion semen diluent, Kenney’s Modified Tyro<strong>de</strong>’s 1<br />
(KMT) Medium, for handl<strong>in</strong>g and process<strong>in</strong>g stallion sperm prior to<br />
flow cytometric sex-sort<strong>in</strong>g.<br />
Methods and Materials Two ejaculates were collected from each of<br />
three Standardbred stallions. Each ejaculate was diluted <strong>in</strong> traditional<br />
KMT that conta<strong>in</strong>ed skim milk or KMT without skim milk and<br />
supplemented with 0.25mg per ml of either bov<strong>in</strong>e serum album<strong>in</strong><br />
(BSA), β-lactoglobul<strong>in</strong>, glyc<strong>in</strong>e or fetal bov<strong>in</strong>e serum (FBS). Diluted<br />
semen samples were <strong>in</strong>cubated at 34ºC or 5ºC. Subjective motility,<br />
acrosome <strong>in</strong>tegrity and morphology were assessed over a 24 h period.<br />
Results and Discussion After <strong>in</strong>cubation for 3 h at 34ºC, there was no<br />
significant difference between the diluents for total motility,<br />
progressive motility or acrosome status of spermatozoa. After<br />
<strong>in</strong>cubation for 24 h at 34ºC <strong>in</strong> KMT, there was a lower <strong>in</strong>ci<strong>de</strong>nce of<br />
sperm tail abnormalities compared with the other diluents (9.3 vs.<br />
23.2 – 24.5 %; p