Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
102 Poster Abstracts<br />
P223<br />
Effect of a steroidal anti-<strong>in</strong>flammatory drug on the<br />
viability of equ<strong>in</strong>e semen cooled for 24 hours<br />
Fioratti, EG*; Melo, CM; Villaver<strong>de</strong>, AISB; Papa, FO; Alvarenga, MA<br />
Department of Animal <strong>Reproduction</strong> and Veter<strong>in</strong>ary Radiology - Faculty of<br />
Veter<strong>in</strong>ary Medic<strong>in</strong>e and Animal Science, UNESP, Botucatu, Brazil<br />
Post-breed<strong>in</strong>g endometritis <strong>in</strong> mares is related to exacerbated<br />
<strong>in</strong>flammatory process, which is stimulated by the presence of sperm<br />
cells <strong>in</strong>si<strong>de</strong> the uterus lead<strong>in</strong>g to the major cause of <strong>in</strong>fertility. The use<br />
of ecbolic drugs associated with uter<strong>in</strong>e flush<strong>in</strong>g and AI with reduced<br />
sperm quantity provi<strong>de</strong>s the most common treatment. Based on<br />
efficiency of steroidal anti-<strong>in</strong>flammatory drugs <strong>in</strong> m<strong>in</strong>imiz<strong>in</strong>g uter<strong>in</strong>e<br />
fluid accumulation by its systemic adm<strong>in</strong>istration and its possible<br />
<strong>in</strong>tra-uter<strong>in</strong>e use, the present study aimed to evaluate the effect of<br />
add<strong>in</strong>g <strong>de</strong>xamethasone to the exten<strong>de</strong>r on equ<strong>in</strong>e sperm viability<br />
dur<strong>in</strong>g two hours of <strong>in</strong>cubation at 37°C and after a 24 hours cool<strong>in</strong>g<br />
period. Four stallions from different breeds were collected twice us<strong>in</strong>g<br />
an artificial vag<strong>in</strong>a, obta<strong>in</strong><strong>in</strong>g a total of eight ejaculates. After<br />
evaluation of sperm motility and concentration, two aliquots<br />
conta<strong>in</strong><strong>in</strong>g 800 x 10 6 of viable sperm were diluted <strong>in</strong> a milk-based<br />
exten<strong>de</strong>r (Botu-Semen®) reach<strong>in</strong>g a volume of 15 mL for each<br />
previous diluted aliquot. These samples were re-diluted with 15 mL of<br />
Botu-semen® non-supplemented (control group) or supplemented<br />
with 2 mg of <strong>de</strong>xamethasone (treatment group), result<strong>in</strong>g <strong>in</strong> a f<strong>in</strong>al<br />
concentration of approximately 0.067 mg/ mL. One part of the<br />
samples from both groups were cooled at 5°C for 24 hours <strong>in</strong> an<br />
equ<strong>in</strong>e semen transport box (Botuta<strong>in</strong>er®) and the other part was<br />
<strong>in</strong>cubated <strong>in</strong> a dry-block at 37°C dur<strong>in</strong>g two hours. Sperm analyses<br />
were performed at 0, 30, 60 and 120 m<strong>in</strong>utes follow<strong>in</strong>g dilution and<br />
after a cool<strong>in</strong>g period of 24 hours us<strong>in</strong>g CASA and a comb<strong>in</strong>ation of<br />
fluorescent probes to assess plasma (Iodi<strong>de</strong> Propidium) and acrosomal<br />
(FITC-PSA) membrane <strong>in</strong>tegrity and mitochondrial transmembrane<br />
potential (JC-1). Compar<strong>in</strong>g both groups among all evaluated<br />
<strong>in</strong>cubation moments, no significant difference (p < 0.05) was<br />
observed for total motility, plasma membrane <strong>in</strong>tegrity and<br />
mitochondrial transmembrane potential. Progressive motility and<br />
percentage of rapid spermatozoa values were higher (p < 0.05) <strong>in</strong> the<br />
control group. The treatment group showed lower (p < 0.05) values<br />
for VAP at 30 and 60 m<strong>in</strong>utes and higher (p < 0.05) values for<br />
percentage of acrosomal membrane <strong>in</strong>tegrity at 0 and lower (p < 0.05)<br />
at 60 m<strong>in</strong>utes of <strong>in</strong>cubation compared to the control group. The results<br />
obta<strong>in</strong>ed <strong>in</strong> 24 hours cooled samples were not different (p < 0.05)<br />
between both groups for all parameters mentioned above, except for<br />
VAP, which was lower (p < 0.05) for the treatment group. In<br />
conclusion, although the treatment group exhibited poor sperm<br />
velocity, the steroidal anti-<strong>in</strong>flammatory drug did not impair sperm<br />
viability.<br />
P224<br />
The effect of equ<strong>in</strong>e growth hormone and its <strong>in</strong>teraction<br />
with gonadotrop<strong>in</strong>s, estradiol and fetal calf serum on<br />
cytoskeleton distribution <strong>in</strong> equ<strong>in</strong>e oocytes matured <strong>in</strong><br />
vitro<br />
Gabriel Pereira, G 1 *, Liu, IKM 1 , Carneiro, GF 1 , Pegoraro, LM 2 , Lorenzo, PL 3<br />
1Population Health and <strong>Reproduction</strong>, University of California, Davis, UCD,<br />
United States; 2 Animal <strong>Reproduction</strong>, Temperate Climate Research<br />
Corporation-EMBRAPA, Brazil; 3 Animal Physiology Department, Universidad<br />
Complutense <strong>de</strong> Madrid, Spa<strong>in</strong><br />
Microtubules and microfilaments are cytoskeletal components that<br />
support cell architecture and modulate cyto- and karyok<strong>in</strong>esis. We<br />
hypothesize that the limited success achieved with <strong>in</strong> vitro maturation<br />
(IVM) of horse oocytes is due to abnormalities associated with<br />
cytoskeleton structures. The objective of this research was to<br />
<strong>in</strong>vestigate the effect and the <strong>in</strong>teractions between equ<strong>in</strong>e growth<br />
hormone (eGH), estradiol (E2), gonadotrop<strong>in</strong>s, and fetal calf serum<br />
(FCS) on the IVM and cytoskeleton organization of equ<strong>in</strong>e oocytes.<br />
Equ<strong>in</strong>e oocytes aspirated from each follicle