75 Integrating Membrane Transport with Male Gametophyte ... - TAIR

More documents

Recommendations

Info

353 Natural Variation Studies For Circadian Clock Input Components In Arabidopsis thaliana Eleni Boikoglou 1, 2 , Amanda Davis 1 , Ferenc Nagy 2 , Seth Davis 1 1 Max Planck Institute for Plant Breeding Research, Koeln, 50829, Germany, 2 Institute of Plant Biology, Biological Research Center, H-6701, Szeged, Hungary The circadian clock is an internal mechanism that regulates various processes in the plant life cycle. The clock is entrained by environmental cues, such as light and temperature. Although light input has extensively been studied, temperature’s role as a clock input signal is as yet unraveled. Recently, studies on circadian mutants have shown that temperature cycles are capable to entrain the clock. Our interest is to dissect the genes that function in this thermo-sensory pathway. Presumably, the genetic architecture of thermo-perception will be similar to, but different from, photo-perception. In this study, natural variation is exploited with respect of thermal entrainment of the circadian clock. Arabidopsis thaliana accessions and Recombinant Inbred Lines (RILS) were transformed using CCR2::LUC reporter. The CCR2 promoter was chosen as its expression is under clock control, and moreover, is thermally regulated. Clock characteristic-traits such as period, phase, and amplitude will be assessed by measuring bioluminescent rhythms of CCR2 transcriptional outputs. Our aim is to identify, map, and characterize temperature-specific genetic components responsible for regulation of the circadian clock. Preliminary results of various lines have revealed that there is low variation within the RILS and high variation amongst them. Thus, it is feasible the scoring of the clock traits will contribute to QTL identification. The identification of these genetic components will lead towards an exciting entry in the temperature entrainment of the circadian clock. 354 Prevalence and mechanisms of F1 incompatibility in Arabidopsis thaliana Kirsten Bomblies, Janne Lempe, Christa Lanz, Norman Warthmann, Detlef Weigel Max Planck Institute for Developmental Biology Postzygotic reproductive incompatibility, that is, the sterility or inviability of hybrids, dramatically illustrates breakdown of gene coordination within a genome and is likely important in speciation. We show here that post-zygotic incompatibilty exists among wild strains of Arabidopsis thaliana. Among over 300 hybrid combinations we identified five F1 hybrids that show environmentally sensitive morphological defects, ranging in severity from leaf chlorosis and twisting, to dwarfism, loss of apical dominance, severe leaf defects and growth arrest. The genetic interactions conform to predictions of the Dobzhansky-Muller model for post-zygotic incompatibility involving two dominant loci. All five hybrids are specific; crosses with other ecotypes did not produce abnormalities. Furthermore, hybrid combinations among the parents of these hybrids show that different genes or alleles underlie the F1 phenotypes. Using micro-arrays, histological and other molecular approaches, we found that these hybrids mount autoimmune responses in the absence of pathogen challenge. Consistent with this, one of two causal regions fine-mapped in one hybrid contains two polymorphic TIR-NBS-LRR class resistance (R) genes. We are now testing candidate genes using artificial micro-RNAs and are mapping the causal loci for the remaining four hybrids. Intriguingly, our Arabidopsis hybrid incompatibility phenotypes (including temperature sensitivity) are remarkably similar to a common F1 hybrid syndrome, hybrid necrosis or weakness, which occurs in a wide variety of other plant species. Based on our data, we hypothesize that rapidly-evolving R genes evolve aberrant interactions with gene variants present in conspecific individuals, resulting in hybrid incompatibility at detectable frequency in Arabidopsis and other plant species. This parallels findings in animals that implicate rapidly evolving genes in reproductive isolation and has important implications for plant evolution and speciation.
355 Phylogenetic Analysis of Arabidopsis PcG Protein EMF2 Lingjing Chen, Renee Sung Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 Arabidopsis Polycomb group (PcG) genes regulate development via repression of distinct MADS-box genes. Arabidopsis proteins EMBRYONIC FLOWER2 (EMF2), VERNALIZATION2 (VRN2), and FERTILIZATION INDEPENDENT ENDOSPERM2 (FIS2) share similar VEF domain to Drosophila PcG protein Su(z)12. Here we report that VEF-domain-containing genes extensively exist in angiosperm, while being a small gene gamily in each species. Three families were recognized based on their domain organization, with EMF2 family that includes EMF2 and VRN2 as the biggest family. Widespread distribution of EMF2 homologs in angiosperm indicates that their early version occurred before dicots and monocots diverged. Global homology strongly suggests that VRN2 subfamily is an abbreviated version from an ancestral EMF2. The members of VRN2 subfamily occur in Salicaceae, Leguminosae, Brassicaceae, and Solanaceae, all members of eudicots, suggesting that they split from EMF2 lineage at a later stage in angiosperm evolution. The INDEL sequences of current members of EMF2 family showed a closest relationship between Nuphar and basal monocot Acorus, as well as Yucca and Asparagus, supporting a Nyphaeaceae-related origin of Monocots. The unique domain organization in FIS2 and VEF-L36 families suggests that they belong to other lineages in VEF superfamily. Intragenic sequence duplication, deletion/insertion, and intergenic exon shuffling found in these three families should account for their functional innovation. 356 Genetic Mechanisms of Seasonal Maternal Effects on Germination Phenology Kathleen Donohue, George Chiang, Deepak Barua, Colleen Colleen, Shane Heschel Harvard University Germination is influenced by seasonal environmental factors experienced during seed maturation on the maternal plant. Both the photoperiod and the temperature during seed maturation alter germination responses to post-dispersal conditions. The response of phytochrome mutants to cool maturation temperatures differed from that of the wild-type, indicating a role of phytochrome in maternal temperature effects on germination. NILs containing different natural allelic variants of the flowering-time gene, FLC, also differed in their germination response to maternal temperature. NILs containing different natural variants of a known dormancy gene, DOG1, differed in their germination response to maternal photoperiod. These are among the first genes to be associated with maternal seasonal effects on germination. Results suggest that genes known to be involved in the regulation of flowering time—phytochromes and FLC—are also likely to be involved in regulating germination phenology, and that natural variation exists for germination responses to maternal seasonal factors that vary with flowering time.
Page 1 and 2:
75 Integrating Membrane Transport w
Page 3 and 4:
79 PREP: Partnership for Research a
Page 5 and 6:
83 Characterization of Orthologous
Page 7 and 8:
87 High-density Arabidopsis Protein
Page 9 and 10:
91 Approaches to Study Homologous R
Page 11 and 12:
95 Predicting the Redundome: A Geno
Page 13 and 14:
99 ReIN: an interactive tool to cre
Page 15 and 16:
103 Proteomic services at the Europ
Page 17 and 18:
107 Ubiquitin Lys 63 Chain Forming
Page 19 and 20:
111 Characterization of the Anti-mi
Page 21 and 22:
115 Molecular Genetic Analysis of P
Page 23 and 24:
119 CLB19, a Novel Pentatricopeptid
Page 25 and 26:
123 The Arabidopsis CDC48 Adapter P
Page 27 and 28:
127 AtCKT1, a Novel Transporter for
Page 29 and 30:
131 Sub-Cellular Localization of DR
Page 31 and 32:
135 Discovering the function of WAV
Page 33 and 34:
139 WRINKLED1, an AP2/EREBP Class T
Page 35 and 36:
143 The Ubiquitin-Specific Protease
Page 37 and 38:
147 Systemic signal transduction of
Page 39 and 40:
151 Dissection of Flowering Pathway
Page 41 and 42:
155 Dissecting genetic pathways und
Page 43 and 44:
159 HANABA TARANU (HAN) Organizes A
Page 45 and 46:
163 What are SCAMPS doing in plants
Page 47 and 48:
167 Analysis of DNA methylation and
Page 49 and 50:
171 Identification of TIA as a Myb-
Page 51 and 52:
175 DEMETER DNA Glycosylase Regulat
Page 53 and 54:
179 Investigation Of The Connection
Page 55 and 56:
183 Genetic Analysis of Vascular Pa
Page 57 and 58:
187 Arabidopsis BRANCHED genes are
Page 59 and 60:
191 Regulation Of BDL Gene Expressi
Page 61 and 62:
195 ARROW1 (ARO1), a Novel Regulato
Page 63 and 64:
199 The Trichome Pock Phenotype of
Page 65 and 66:
203 Control of Leaf Vascular Patter
Page 67 and 68:
207 FAMA Controls The Switch Betwee
Page 69 and 70:
211 Subtilisin-like serine protease
Page 71 and 72:
215 TRIDENT and VARICOSE encode com
Page 73 and 74:
219 Analysis of a Mutant, #1-63, wh
Page 75 and 76:
223 Quantitative Trait Analysis of
Page 77 and 78:
227 LEAFY and the Evolution of Rose
Page 79 and 80:
231 Overexpression of Arabidopsis S
Page 81 and 82:
235 Ethylene Signaling Components A
Page 83 and 84:
239 Accumulation of Reactive Oxygen
Page 85 and 86:
243 Intracellular Production Of Rea
Page 87 and 88:
247 Large-Scale Screen and Isolatio
Page 89 and 90: 251 Ontogeny of the Arabidopsis Cir
Page 91 and 92: 255 Cell type-specific expression a
Page 93 and 94: 259 Characterization of Flowering T
Page 95 and 96: 263 Involvement of Cytokinins and T
Page 97 and 98: 267 Identification of new actors of
Page 99 and 100: 271 Scarecrow-like Protein SCL14 In
Page 101 and 102: 275 Protein Prenylation Is Required
Page 103 and 104: 279 Mechanisms controlling coordina
Page 105 and 106: 283 GLZ1, a member of family 8 glyc
Page 107 and 108: 287 Arabidopsis as a Model System t
Page 109 and 110: 291 The NIMIN-NPR1 Connection: A Mo
Page 111 and 112: 295 A Search for Transcriptional Re
Page 113 and 114: 299 Identification of genes contrib
Page 115 and 116: 303 Regulation of AGAMOUS Expressio
Page 117 and 118: 307 Genetic Control of the Interplo
Page 119 and 120: 311 Centromeric Retrotransposons: P
Page 121 and 122: 315 Finding Intron Sequences That E
Page 123 and 124: 319 Biochemical Characterization Of
Page 125 and 126: 323 Fluorescent amino acid sensors
Page 127 and 128: 327 The Role of Tryptophan Metaboli
Page 129 and 130: 331 A Putative Bifunctional Wax Est
Page 131 and 132: 335 Analysis of the Complex BIO3 /
Page 133 and 134: 339 Exploring of Arabidopsis non-ho
Page 135 and 136: 343 A Yeast-2-Hybrid Assay Reveals
Page 137 and 138: 347 Genetic Mechanisms of Glucosino
Page 139: 351 Potent Induction of flowering b
Page 143 and 144: 359 eQTL-mapping reveals AMP2A, a c
Page 145 and 146: 363 Progress Towards the Cloning of
Page 147 and 148: 367 Natural Variation in Infloresce
Page 149 and 150: 371 Natural Variation for Seed Dorm
Page 151 and 152: 375 Natural genetic and epigenetic
Page 153 and 154: 379 SPIKE1 is a guanine nucleotide
Page 155 and 156: 383 The RAD23 Family of Ubiquitin-L
Page 157 and 158: 387 Molecular Functions of ARL2 and
Page 159 and 160: 391 Characterization of the Sugar-I
Page 161 and 162: 395 Functional analysis of phosphat
Page 163 and 164: 399 Identification and Characteriza
Page 165 and 166: 403 Association and Localization of
Page 167 and 168: 407 Functional Requirements for PIF
Page 169 and 170: 411 Using High-throughput Chemical
Page 171 and 172: 415 The F-box Protein PPS Functions
Page 173 and 174: 419 Round The Clock - The Molecular
Page 175 and 176: 423 Protein Prenylation Process Neg
Page 177 and 178: 427 Integration of light and abscis
Page 179 and 180: 431 Functional analysis of ROP10 sm
Page 181 and 182: 435 The Importance Of RecQ Like Hel
Page 183: 439 A Comparison of Full Versus Par
show all

75 Integrating Membrane Transport with Male Gametophyte ... - TAIR

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?