75 Integrating Membrane Transport with Male Gametophyte ... - TAIR

More documents

Recommendations

Info

285 Screening for Suppressors of Arabidopsis acd11 Frederikke Malinovsky 1 , Peter Brodersen 2 , Daniel Hofius 1 , Lea McKinney 1 , Nikolaj H Petersen 1 , Morten Petersen 1 , Berthe Fiil 1 , John Mundy 1 1 institue of molecular biology, university of copenhagen, 2 Institut de Biologie Moleculaire des Plantes du CNRS Despite attempts to link plant cell death to animal apoptosis, comparative analyses of the genetic determinants of programmed cell death (PCD) in plants and animals have yet to identify conserved gene functions. Loss of function of the Arabidopsis gene Accelerated Cell Death 11 (ACD11; Brodersen et al. 2002 Genes & Develop. 16, 490; Brodersen et al. 2005 Plant Physiol. 138, 1037) activates vegetative cell death and disease resistance responses dependent upon the hormone salicylate (SA). Double mutant analysis showed that of 12 mutants affected in defense-associated PCD (eds1, pad4, eds5, sid2, npr1, rar1, pbs1, pbs3, ein2, etr1, jar1, ndr1), only two (eds1 and pad4) suppress acd11 PCD in the presence of the SA analog BTH. To further understand ACD11 function, we are searching for PCD regulators in a largescale acd11 suppressor screen. More specifically, acd11 homozygotes expressing the bacterial SA hydroxylase nahG were mutagenized and plants surviving BTH treatment isolated as putative suppressors. This identified a number of recessive and dominant suppressors of acd11. Recent work has sorted the recessive mutants fall into fifteen complementation groups. Sequencing has confirmed that two of these groups are allelic to eds1 validating the utility of the screen. 286 Analysis of a nonpathogenic strain of Pseudomonas syringae for use in host range and pathogenicity studies on Arabidopsis thaliana and tomato Toni Mohr 1 , Ryan Anderson 1 , Peter Bowerman 1 , Nina Long 1 , Leiya Williams 1 , Joanna Jelenska 2 , Jose Castillo 2 , Jean Greenberg 2 , Boris Vinatzer 1 1 Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Fralin Biotechnology Center, West Campus Drive, Blacksburg, VA 24061-0346, USA, 2 Department of Molecular Genetics and Cell Biology, The University of Chicago, 1103 East 57th Street, EBC410, Chicago, IL 60637, USA The bacterial plant pathogen Pseudomonas syringae encompasses over 50 pathovars that collectively cause disease on hundreds of plant species. However, any particular strain is limited to one or a few plant hosts. This host range is determined by numerous bacterial effector proteins, which are injected into a plant cell by a Type III secretion system (TTSS). We are examining how TTSS effector proteins affect the host range of Arabidopsis and tomato pathogens. Here we characterize a nonpathogenic strain, Psy508, originally isolated as a biocontrol strain against the apple scab fungus. Although closely related to the bean pathogen PsyB728a, Psy508 is unable to cause disease on any tested plant species. Interestingly, Psy508 does not appear to have a functional TTSS, which is essential for pathogenicity. The typical P. syringae hrp/hrc cluster with flanking effector loci coding for the TTSS machinery and effectors is absent in this strain, and has been replaced by a bacteriophage sequence. Additionally, Psy508 appears to be missing most effector genes elsewhere in the genome. We also sequenced three genomic islands that contain effectors in PsyB728a; these islands are either rearranged, or lack effector genes in Psy508. Psy508 may thus be a useful strain in which to study the effects of individual effectors, or combinations of effectors, isolated from other strains with different host ranges. We are currently cloning the TTSS, without the flanking effector loci, from PsyB728a. By adding this TTSS and individual effectors to Psy508, we plan to analyze the role of effector proteins in determining the host range of Pseudomonas syringae.
287 Arabidopsis as a Model System to Study Plant Defense Against Fusarium graminearum, the Causative Agent of Scab in Wheat and Barley Vamsi Nalam, Ragiba Makandar, Darcy Maier, Harold Trick, Jyoti Shah Kansas State University Fusarium head blight (FHB) is a common and devastating disease of wheat and barley around the world. In the US, yield losses due to FHB in some years have reached $1 billion. Fusarium graminearum Schwabe is the principal causal agent of FHB. Unlike many other diseases, monogenic gene-for-gene resistance to FHB has not been identified and the mechanism(s) involved in signaling and activation of plant defense against F. graminearum are poorly understood. A host-fungus system consisting of Arabidopsis-F. graminearum provides an excellent model system to study and rapidly identify genes involved in signaling and activation of plant defenses. We have observed that constitutive overexpression of the Arabidopsis NPR1 (AtNPR1) gene confers enhanced resistance against F. graminearum in Arabidopsis and wheat (Makandar et al. 2006), suggesting conservation of defense mechanisms against this pathogen in Arabidopsis and wheat. Furthermore, SA and BTH application also enhance resistance against this fungus. Our results indicate that constitutive expression of AtNPR1 primes wheat defenses to respond faster to SA and the fungus. Our studies in Arabidopsis have identified other components of host defense against F. graminearum. In addition, we have identified an Arabidopsis gene, which contributes to F. graminearum virulence. Progress on this work will be presented. Makandar, R., Essig, J. S., Schapaugh, M. A., Trick, H. N. and Shah, J. 2006. Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1. Mol. Plant-Microbe Interact. 19:123-129. 288 Plant Growth and Pathogen-Related Response Are Regulated via Glutathionylation of a Single Protein Ken'ichi Ogawa 1, 2 , Masayoshi Matsumoto 1, 2 , Hatano-Iwasaki Aya 1, 2 , Kenji Henmi 1, 2 1 RIBS OKAYAMA (Res. Inst. Biol. Sci. Okayama), 2 CREST, JST We have recently been reporting that glutathione (GSH), an abundant antioxidant, regulates many physiological events in plants [Ogawa (2005) Antioxid. Redox Signal. 7: 973-981]. Since most of the events that are governed by GSH cannot be controlled by other thiols, we focused on and studied the physiological function of proteins undergoing glutathionylation (covalent biding of the GSH moiety through the disulfide bride). Here I will present a talk showing that one of proteins undergoing glutathionylation in chloroplasts plays a key role in plant growth and pathogen-related response and that glutathionylation determines the function of the protein. Considering that GSH synthesis is strongly dependent on photosynthesis and that plant growth and pathogen-related response are regulated by GSH, it can be concluded that plant growth and pathogen-related response are regulated by photosynthesis via glutathionylation of the single protein.
Page 1 and 2:
75 Integrating Membrane Transport w
Page 3 and 4:
79 PREP: Partnership for Research a
Page 5 and 6:
83 Characterization of Orthologous
Page 7 and 8:
87 High-density Arabidopsis Protein
Page 9 and 10:
91 Approaches to Study Homologous R
Page 11 and 12:
95 Predicting the Redundome: A Geno
Page 13 and 14:
99 ReIN: an interactive tool to cre
Page 15 and 16:
103 Proteomic services at the Europ
Page 17 and 18:
107 Ubiquitin Lys 63 Chain Forming
Page 19 and 20:
111 Characterization of the Anti-mi
Page 21 and 22:
115 Molecular Genetic Analysis of P
Page 23 and 24:
119 CLB19, a Novel Pentatricopeptid
Page 25 and 26:
123 The Arabidopsis CDC48 Adapter P
Page 27 and 28:
127 AtCKT1, a Novel Transporter for
Page 29 and 30:
131 Sub-Cellular Localization of DR
Page 31 and 32:
135 Discovering the function of WAV
Page 33 and 34:
139 WRINKLED1, an AP2/EREBP Class T
Page 35 and 36:
143 The Ubiquitin-Specific Protease
Page 37 and 38:
147 Systemic signal transduction of
Page 39 and 40:
151 Dissection of Flowering Pathway
Page 41 and 42:
155 Dissecting genetic pathways und
Page 43 and 44:
159 HANABA TARANU (HAN) Organizes A
Page 45 and 46:
163 What are SCAMPS doing in plants
Page 47 and 48:
167 Analysis of DNA methylation and
Page 49 and 50:
171 Identification of TIA as a Myb-
Page 51 and 52:
175 DEMETER DNA Glycosylase Regulat
Page 53 and 54:
179 Investigation Of The Connection
Page 55 and 56: 183 Genetic Analysis of Vascular Pa
Page 57 and 58: 187 Arabidopsis BRANCHED genes are
Page 59 and 60: 191 Regulation Of BDL Gene Expressi
Page 61 and 62: 195 ARROW1 (ARO1), a Novel Regulato
Page 63 and 64: 199 The Trichome Pock Phenotype of
Page 65 and 66: 203 Control of Leaf Vascular Patter
Page 67 and 68: 207 FAMA Controls The Switch Betwee
Page 69 and 70: 211 Subtilisin-like serine protease
Page 71 and 72: 215 TRIDENT and VARICOSE encode com
Page 73 and 74: 219 Analysis of a Mutant, #1-63, wh
Page 75 and 76: 223 Quantitative Trait Analysis of
Page 77 and 78: 227 LEAFY and the Evolution of Rose
Page 79 and 80: 231 Overexpression of Arabidopsis S
Page 81 and 82: 235 Ethylene Signaling Components A
Page 83 and 84: 239 Accumulation of Reactive Oxygen
Page 85 and 86: 243 Intracellular Production Of Rea
Page 87 and 88: 247 Large-Scale Screen and Isolatio
Page 89 and 90: 251 Ontogeny of the Arabidopsis Cir
Page 91 and 92: 255 Cell type-specific expression a
Page 93 and 94: 259 Characterization of Flowering T
Page 95 and 96: 263 Involvement of Cytokinins and T
Page 97 and 98: 267 Identification of new actors of
Page 99 and 100: 271 Scarecrow-like Protein SCL14 In
Page 101 and 102: 275 Protein Prenylation Is Required
Page 103 and 104: 279 Mechanisms controlling coordina
Page 105: 283 GLZ1, a member of family 8 glyc
Page 109 and 110: 291 The NIMIN-NPR1 Connection: A Mo
Page 111 and 112: 295 A Search for Transcriptional Re
Page 113 and 114: 299 Identification of genes contrib
Page 115 and 116: 303 Regulation of AGAMOUS Expressio
Page 117 and 118: 307 Genetic Control of the Interplo
Page 119 and 120: 311 Centromeric Retrotransposons: P
Page 121 and 122: 315 Finding Intron Sequences That E
Page 123 and 124: 319 Biochemical Characterization Of
Page 125 and 126: 323 Fluorescent amino acid sensors
Page 127 and 128: 327 The Role of Tryptophan Metaboli
Page 129 and 130: 331 A Putative Bifunctional Wax Est
Page 131 and 132: 335 Analysis of the Complex BIO3 /
Page 133 and 134: 339 Exploring of Arabidopsis non-ho
Page 135 and 136: 343 A Yeast-2-Hybrid Assay Reveals
Page 137 and 138: 347 Genetic Mechanisms of Glucosino
Page 139 and 140: 351 Potent Induction of flowering b
Page 141 and 142: 355 Phylogenetic Analysis of Arabid
Page 143 and 144: 359 eQTL-mapping reveals AMP2A, a c
Page 145 and 146: 363 Progress Towards the Cloning of
Page 147 and 148: 367 Natural Variation in Infloresce
Page 149 and 150: 371 Natural Variation for Seed Dorm
Page 151 and 152: 375 Natural genetic and epigenetic
Page 153 and 154: 379 SPIKE1 is a guanine nucleotide
Page 155 and 156: 383 The RAD23 Family of Ubiquitin-L
Page 157 and 158:
387 Molecular Functions of ARL2 and
Page 159 and 160:
391 Characterization of the Sugar-I
Page 161 and 162:
395 Functional analysis of phosphat
Page 163 and 164:
399 Identification and Characteriza
Page 165 and 166:
403 Association and Localization of
Page 167 and 168:
407 Functional Requirements for PIF
Page 169 and 170:
411 Using High-throughput Chemical
Page 171 and 172:
415 The F-box Protein PPS Functions
Page 173 and 174:
419 Round The Clock - The Molecular
Page 175 and 176:
423 Protein Prenylation Process Neg
Page 177 and 178:
427 Integration of light and abscis
Page 179 and 180:
431 Functional analysis of ROP10 sm
Page 181 and 182:
435 The Importance Of RecQ Like Hel
Page 183:
439 A Comparison of Full Versus Par
show all

75 Integrating Membrane Transport with Male Gametophyte ... - TAIR

Create successful ePaper yourself

Delete template?

Save as template?