cifri annual report 2007- 2008 - Central Institute of Brackishwater ...

A spectrophotomctric procedure for estimation of
phenol in bacterial broth culture has becn
The IINA was also used to amplify the mitochoncirial
DNA genes. Scvcral genes were atnplified,
standardized. Standard curve of phcnol for C'ytochronie b gene, C'ytochrome oxidnsc II. 16s
photometric cstimation of phenol was drawn. Tlirec
bacterial strains wcre examined and were found to
degrade plicnol and pentachlorophcnol. to different
extents.
rKNA gene. The C\~toc*lit-ornc~ h gene :~mpIification
resulted in a a11.lplilication fragmetit of 360 bp. This
360 kp DNA fragment fiom P. ticeto, .Y(*tlc~todoll
c.nnc-ila and Gudlisi~t c-/irrpr.cl were seclucnccd.
Strcss metliatctl gcncfic xltcration in fish
Assessment of gcnctic changes were investigated in
J'trtt tiu,v ticsro, A'~~~tcrtrot/otr c*trt~c.iln, and Gc~dzi.vi(r
c*kap+cr samples fio~n different locations on the rivcr
Damodar representing tlic uy)strearn reference site with
low pollution. Industrit~l polluted sitc arid downstream
of' industrial sitc. 'The 4 RAPD-PCR oligo-dcgcticrate
primers wcrc uscd for amplification of'gcnomic DNA.
Each prinier was found to be highly polymorphic and
the number of fragments amplified was large. l'hc
RAPD patterns wcrc gct~cr;~tccl for all tlic sarnplcs
collected fiom the above-mentioned locations. Tlicsc
fragment pattenis wcrc then analyzcd for tlic prcscncc
and absence of'amplificd bands.
Tahlc 8 : Primer Sequences
Primcrs Sequence 5'- 3' %GC contcnt
i.'ish prolcomics strrdy
'T'issuc proteins viz. scrurn, ~nusclc, lens anti KHC'
mcmbrane of fish Ritu ritu wcre sutjcctcd to
proteomic analysis. Ritc~ rittr wcrc collcc~ed fj.0111 tlic
local markets live. Scrull.1, muscle, and lens prolcins
were analyzed by onc-ditncnsional sodium dodecyl
anellis polyilcrylamidc gel electrophoresis ( I -rl (-;I:)
and protcin profiles werc gcncratcd. 'Tht~s~: tissuc
proteins wcrc also analyzcd by 2-dimvnsional
polyac~ylamidc gcl electrophoresis (2-D GE) fiv
generating protcorric maps.
In the river [lamotfar niusclc ancl lens protcins of.4.
.vc~cti~rtlr~rla wcre analyzed by I- and 2- D GE and
protein profilcs wcrc gcnel-atcd. Imagc irnalysis is
being carried out.
Protein profilc of fish mucus was dctcr~~~irrcd tty SDS-
PAGE for idcntifica~ivn of antibacterial proteins. Tlic
mctliod is fi~rthcr st;rndal-dizcd for rc~noval of'
hindering f'actors like lipid, carboliydlates anrl nonsolublc
protein moieties.
PCR was carricd out in 25 1
reaction mixturc
containing 100 ng DNA, 100 pM of decamer primer,
10 mM dNTP mixture (Dynazymc), 10 X PCR bufTer
(Dynazymc), 1.5 mM MgCl, and 0.05 U of Xlq
polymerases (Dynazymc).
Fig. 7 : Sl!S-l'.\( ;I I",, I'~-olilc jlcl~tidcs fro~ri
sAlri nillctls of'conttx)l fi!,ll