cifri annual report 2007- 2008 - Central Institute of Brackishwater ...
cifri annual report 2007- 2008 - Central Institute of Brackishwater ...
cifri annual report 2007- 2008 - Central Institute of Brackishwater ...
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Exposure <strong>of</strong> the Fish to envirunmcntal pollutant in<br />
iaboratorics for gene spsc~lic tranicrlpt gene<br />
expression was donc /.uhpo milira young fi*hci wcrc<br />
cxposcd to three diffcrcnt concentrations <strong>of</strong>cadni~um<br />
dirsolvcd In 20 liters <strong>of</strong> water alunp with a ci~ntrol<br />
expei>mcnt In whicli the cadmtum wus not added.<br />
Flshes uerr exposcd to 3 dlffcrcnt concentrdaons. I<br />
mgl I. 5 n~gl and 10 mgl ' Tu'o dlfferenl sel or<br />
melallulhionrin apcc~fic gcnc prtmcrs were ulllired<br />
for delecttun ui the exprcwnn <strong>of</strong> TWO di:fercnt<br />
~s<strong>of</strong>urms oimelulluthionrtn pctlcc Thc control fishcs<br />
dld no1 express thr metdlluth~oncin genci as tndicatcd<br />
by ahsence <strong>of</strong> cDNA ampl~ficatton prnduct<br />
Detcctahle gene expresston <strong>of</strong> only one ia~funn <strong>of</strong><br />
inctal;othtonc~n gene was obren,od<br />
Protctns froin foot ttssucs <strong>of</strong> pastropuds (B(~l1arnin<br />
he,igii/p,lsir and Pvh g/mh,riel and foot, gill and<br />
tnantlc tissuc5 <strong>of</strong> thc h~vsivcs (/.rim~ilidr,~r<br />
n~rn~gi,io/i~l ~niolluscs collected from dlfiercnl ritrs ui<br />
thc rlvcr Datnodarwere analyred h one-d~mensiondl<br />
iodiuln dodccyl anell~s polyacryli~m~de gel<br />
elcctrophorcris iorthetrproteinpr<strong>of</strong>ilesanalyr~s.<br />
Experiment wcre conducted ill laboratory *~th<br />
lingerlings <strong>of</strong>Lohrr, wiiiio kept In 30 hrrc jar unteruf<br />
pH 7 8-8 3, rlkailn~ty 1x0-23Umyl and ihardncsr IXO-<br />
200 tngl . Thty were cxpnlcd for 30 dab, lo sub Icrhsl<br />
copperconcentratlonr(0 02 mgl. 0.01 nlgl .0.04 lnpl<br />
and 0.05 mel 1. The rcrults Indic,bte 11ui1 tlte<br />
finpcrlings <strong>of</strong>I.uheti nililie did inut cxh~h~t r~pn>lic.inl<br />
phystological changes ~n terms uiIli~crnaglnb~nti.(1.<br />
8.0), blood pH(h 8-7 0). Cholcrturol (lSX.227<br />
mgidL).Glucosci27.5h1~g~dl) .Tri~I>~cr~de(Sh 6-54<br />
mg'dL) and Control (54-93np;mli O~,tl~ib~ilg IIIC<br />
hon~eustrs~~ui lish<br />
The inelel cmntetit ill uatcr saniplcs cxh~bited ,~hr~~icu<br />
ufrny appl.cciablc atnnunt <strong>of</strong>thc \tud~i'cl mcl.lli (Cci.<br />
Cu. Mn. Ph and Za) Prosence or Mn 'lnd Zit war<br />
noticed ti1 unt; froill 1)urgapur arcs uniy ~n oar<br />
aampltng<br />
ILlkc water, the lneliil conlcnt in liill flcsh ~amplc~<br />
exhtbited absence <strong>of</strong> ni:) apprcc~ahlc amo'unl oi tile<br />
studied mtltiia(Cd. Cu. Mn, PhandZii) Only eonlent<br />
olZn wasnoticed in lish fleshup to5 Rpprn
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