automated blood sampler - Instech Laboratories, Inc.

AUTOMATED BLOOD SAMPLER
for
LABORATORY ANIMAL RESEARCH
The equipment behind the science.

Automated blood sampling systems, properly deployed,
can increase the throughput of drug metabolism
and pharmacokinetics research groups. ABS systems
can also improve the accuracy and repeatability of sample
collection, minimize employee exposure to allergens and
reduce the stress on animals caused by handling and restraint.
THREE UNIQUE FEATURES of the INSTECH ABS
1 Undiluted Samples Verified
In-line sensors built into
the Instech ABS distinguish
between pure blood, diluted
blood and IV fluid. Before a
study begins, these sensors
ensure the system is set up
In-line sensor properly to deliver a pure
sample to the collection vial.
Without analytical guidance, the choice of where to
separate the diluted leading edge from the pure sample
is left to chance. (Visual discrimination is particularly
unreliable.) Trimming too much can lead to wasted
blood and overly long sample collection times; too
little, to diluted samples and inaccurate data.
“
In our laboratory,
one technician collects samples
from thirty-six animals simultaneously.
We are no longer a bottleneck in the drug
development process.
“
During a study the sensors serve a dual role. First,
they verify that blood arrives at the sensors at the
correct time in the sampling process. If not, the
culprit is often a catheter blockage; the system will
respond with an auto-retry routine and alert the user.
Second, the sensors measure the blood’s hematocrit,
providing real-time physiologic data.
saline trim volume pure pure blood
Other blood sampling systems either have no sensors
at all or monitor pressure during the process, a parameter
which is not directly related to sample integrity.
By comparison, the Instech ABS monitors the blood in
the line to ensure that the sampler is set up optimally
before a study, respond to blockages during the study
and provide data for analysis afterward.
Measurement of sample dilution as blood
passes through the in-line sensor

2 Direct Path
Peristaltic pumps in the Instech ABS move blood
samples directly from the animal to the vials in
the refrigerated fraction collector. This design has
several advantages over other systems that use a
syringe pump to pull the entire sample back into
reservoir tubing, then push it forward to the
collection vial: (1) the shorter, direct flow path
causes less mixing, which leads to smaller trim
volumes for undiluted samples and in turn to more
samples per animal; (2) sample cycle times are
faster (samples can be taken closer together); and
(3) sample volumes are not limited by the volume
of the reservoir tubing.
Other systems
(syringe pump driven)
syringe
pump
valve
valve
peristaltic
pump
valve
Instech ABS
(peristaltic pump driven)
3 Flexibility
The Instech ABS is designed to work with a wide
range of animals, from mice and rats to large animals.
With mice, the ability to collect small volumes and
minimize waste is critical to avoid excessive hemodilution.
The ABS’s low loss mode returns the blood at
the leading edge, cutting wasted blood from 40-80µl
to just 3µl per sample. Instech Solomon’s tapered 1Fr
FunnelCath and low-torque swivels are also keys to
success with mice.
For large animal sampling, simply switch to larger
catheters and tubing sets. The ABS can even split
a single sample between two collection tubes
when blood is plentiful.
Even if you work only with rats, the Instech ABS is
remarkably flexible. An automated routine calibrates
the system for any catheter you choose. The sampler’s
small size and several mounting options give you many
choices for caging, including metabolic cages. Use a
two channel swivel for simultaneous sampling and
infusion or BP measurement, or even a five-channel
swivel for simultaneous sampling and microdialysis.
Automated sampling is an investment. Make the
most of it by choosing the most flexible sampler.
One machine, many species

Precision Hardware
Integrated fraction collector
stores up to ten samples at
4-6°C. Wells can be reused as
initial samples are removed.
Five precision peristaltic pumps
move blood and IV fluids
in direct paths through the
system without valves.
Withdrawn blood is replaced
with IV fluid. Low ‘KVO’ rate
maintains catheter patency.
In-line sensors help ensure that
samples are taken as planned.
Fluid swivel allows continuous
one, two, or five channel fluid
access to a freely moving animal.
Use caging of your choice for a
familiar, low stress environment.
Slotted cage top slides off
for oral dosing.
Optional stand for rodent
sampling with integrated
counter-balanced lever arm
and IV bag holder. Other
mounting arrangements
are also possible.

Easy-to-Use Software
Control and monitor up to twelve samplers from a single PC
Program the sample times, volumes, rates, and other
study parameters for each sampler independently or
apply a single protocol to all samplers
Calibrate using automated routines that adapt the system
to the catheters and tubing sets that are best suited to your
animal model and experiment

Specifications
Sample vials
Fraction collector type
Sample storage temperature
Maximum number of sampling events
Maximum duration of sampling session
Auto-retry routine
KVO feature for catheter patency
Time to take one sample
Standard 1.5 ml centrifuge tubes
10 well rotary (more than 10 samples possible once initial samples are removed)
4-6° C
15 programmed plus 5 more on demand
Unlimited
Minimum collected sample size ~25 µl
Maximum collected sample size
Max # of 100 µl samples from a rat* 33
Max # of 25 µl samples from a mouse* 12
Dilution of sample
Volume of blood withdrawn per sample
Tee blood sensors
Computer system requirements
Email status notification
Maximum samplers per computer 12
Communication method
Communication cable length
Power supply
Power consumption
Weight
Dimensions (WxDxH)
Skips to next sample if 2 attempts fail to pull in blood
Adjustable from 0-999 µl/hr; 5 µl per pulse
Varies based on parameter settings (typically 3-5 min)
3 ml
None
Programmed vol. plus 40-80 µl borrowed; all but 2-4 µl of excess returned to animal
Electrical impedance technology
PC or laptop with Windows 2000 or XP, >256M RAM
Using SMTP
RS-485 using RS-232 serial port converter; 1000 ft (300m) transmission capability
6 ft (1.8m) standard; other lengths may be ordered
15VDC, 4.6A; 100-240 VAC input, 47-63 Hz
Less than 40W per unit, excluding computer; UPS recommended
7.4 lbs (3.3 kg)
9.5 x 10 x 6.5 in (24 x25x17 cm)
* Based on limit of 15% of animal’s blood volume from NIH Guidelines for Survival Bleeding of Mice and Rats (Feb 2001).
Assumptions: blood volume of 22 ml in 350g rat, 2.2 ml in 30g mouse; trim loss of 3 µl in Low Loss mode.

Ordering Information
AUTOMATED BLOOD SAMPLERS
ABS110S Automated laboratory animal blood sampler - initial unit. Includes sampler unit with 10x1.5ml
refrigerated fraction collector, five peristaltic pumps and power supply. Also includes one complete
tubing set, two sensor tees, RS-232 cable, RS-485 converter and control software.
ABS110 Automated laboratory animal blood sampler - additional units. As above, but without
software or RS-485 converter.
STAND AND TETHERING EQUIPMENT
ABS1/R Elevated rodent stand with counter-balance lever arm for swivel, IV bag holder. Accommodates
standard 10.5”x19”x8” rodent cages.
375/22 Single channel stainless steel swivel, 22ga.
375/D/22 Dual channel stainless steel swivel, 22ga. Extra channel available for IV drug infusion,
BP monitoring or other applications.
375/25 Single channel stainless steel swivel, 25ga.
CM/T143 Slotted stainless steel top for standard 10.5”x19”x8” rodent cages.
SR750 Swivel mount and strain relief for large animals.
VAH95AB Vascular Access Harness for rats, single catheter.
CIH105 Infusion harness for rats, larger ID for two catheters.
CIH62 Infusion harness for mice.
Various Large animal jacket and tether.
CBAS-C30 Optional CBAS heparin coated polyurethane catheter, 3Fr, 60cm, rounded tip.
PU-C30 Optional uncoated polyurethane catheter, 3Fr, 60cm, rounded tip.
PUFC-C20-10 Polyurethane FunnelCath for mice, 2Fr tapering to 1.2Fr, 60cm.
SAMPLER TUBING SETS
ABS1/TS015 Complete tubing set for ABS110. Includes IV Fluid,Sample, Air and Waste pump tube sets,
injector tube segment and waste collection bag. Most segments .015"ID. EtO sterilized.
Does not include tees or catheter.
ABS1/TS031 Complete tubing set for ABS110. Includes IV Fluid, Sample, Air and Waste pump tube sets,
injector tube segment and waste collection bag. Most segments .031"ID. EtO sterilized.
Does not include tees or catheter.

Instech provides direct sales, installation, training and
technical support to customers in the US and Europe.
For more information please contact:
Headquarters
Plymouth Meeting PA USA
European Sales Office
Almere, The Netherlands
TL 800-443-4227
TL +1-610-941-0132
FX +1-610-941-0134
www.instechlabs.com
Authorized ABS Distributors:
Linton Instrumentation
UNITED KINGDOM
TL 44 (0) 1379 65 13 44
FX 44 (0) 1379 65 09 70
mail@lintoninst.co.uk
www.lintoninst.co.uk
Primetech Corporation
Koisikawa Daikoku Bldg. 9F
1-3-25 Koishikawa, Bunkyo-ku
Tokyo 112-0002
JAPAN
TL 81 3 3816 0851
FX 81 3 3814 5080
www.primetech.co.jp
Kuo Yang Scientific Corp.
4f-1, No. 98, Min Chuan Road
Hsin Tien City
Taipei 231
TAIWAN
TL 886 2 22196600
FX 886 2 22191717
www.kuoyang.com
Walker Scientific
P.O. Box 388
Hillarys 6923
WESTERN AUSTRALIA
TL 61 (0) 8 9240 5255
FX 61 (0) 8 9240 5266
gwalker@business.ibc.com.au
Marsap Services Pvt. Ltd.
29 Ujagar Industrial Estate
WTP Marg, Deonar
Mumbai 400088
INDIA
TL 022-25516908
FX 022-25563356
www.marsap.com
ABS110revF.qxp 6/07