Thermo Scientific Pierce Crosslinking Technical Handbook
Thermo Scientific Pierce Crosslinking Technical Handbook Thermo Scientific Pierce Crosslinking Technical Handbook
Appendix I – Structures (continued) Appendix I Structures Product # Product Name Structure Reference 22339 SDA M.W. 225.20 Spacer Arm 3.9 Å O N O N O O SDA NHS-Diazirine Spacer Arm 3.9 Å N 26167 SDAD M.W. 388.46 Spacer Arm 13.5 Å O N O O O S S SDAD NHS-SS-Diazirine Spacer Arm 13.5 Å H N O N N 26169 SBAP • Inman, J.K., et al. (1991). Bioconjug. Chem. 2, 458-463. M.W. 307.10 Spacer Arm 6.2 Å 22419 SFB SBAP M.W. 307.10 Spacer Arm 6.2 Å M.W. 247.20 Spacer Arm 5.8 Å 22411 SHTH M.W. 311.68 Spacer Arm 7.9 Å 22349 SIA M.W. 283.02 Spacer Arm 1.5 Å • Thorpe, P.E., et al. (1984). Eur. J. Biochem 140, 63-71. • Rector, E.S., et al. (1978). J. Immunol. Methods 24, 321-336. SIA M.W. 283.02 Spacer Arm 1.5 Å 22329 SIAB M.W. 402.14 Spacer Arm 10.6 Å • Cumber, A.J., et al. (1985). Methods Enzymol. 112, 207-225. • Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press, pp. 542, 553, 568. 22360 SMCC M.W. 334.32 Spacer Arm 8.3 Å SMCC M.W. 334.32 Spacer Arm 11.6 Å 38 For more information, or to download product instructions, visit www.thermo.com/pierce
Appendix I Structures Product # Product Name Structure Reference 22102 22103 SM(PEG) 2 M.W. 425.39 Spacer Arm 17.6 Å SM(PEG)2 M.W. 425.39 Spacer Arm 17.6 Å 22104 22107 SM(PEG) 4 M.W. 513.5 Spacer Arm 24.6 Å SM(PEG)4 M.W. 513.50 Spacer Arm 24.6 Å [ ] 22105 SM(PEG) 6 M.W. 601.6 Spacer Arm 32.5 Å SM(PEG)6 M.W. 601.60 Spacer Arm 32.5 Å [ ] 22108 SM(PEG) 8 M.W.689.71 Spacer Arm 39.2 Å SM(PEG)8 M.W. 689.71 Spacer Arm 39.2 Å [ ] 22112 22113 SM(PEG) 12 M.W. 865.92 Spacer Arm 53.4 Å SM(PEG)12 M.W. 865.92 Spacer Arm 53.4 Å [ ] 22114 SM(PEG) 24 M.W. 1394.55 Spacer Arm 95.2 Å SM(PEG)24 M.W. 1394.55 Spacer Arm 95.2 Å [ ] 22416 SMPB M.W. 356.33 Spacer Arm 11.6 Å 22363 SMPH M.W. 379.36 Spacer Arm 14.2 Å SMPB M.W. 356.33 Spacer Arm 11.6 Å • Thorpe, P.E., et al. (1984). Eur. J. Biochem 140, 63-71. • Rector, E.S., et al. (1978). J. Immunol. Methods 24, 321-336. SMPH M.W. 379.36 Spacer Arm 14.3 Å To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor. 39
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- Page 3 and 4: Introduction What is crosslinking C
- Page 5 and 6: Crosslinking Applications Crosslink
- Page 7 and 8: Solid-phase immobilization Proteins
- Page 9 and 10: O NH Biotin HN S SO 3 - Cleavable D
- Page 11 and 12: Sulfo-SBED Reagent Thermo Scientifi
- Page 13 and 14: Figure 3. Applications of Sulfo-SBE
- Page 15 and 16: Structure determination with heavy/
- Page 17 and 18: Bioconjugate Toolkit The next gener
- Page 19 and 20: Crosslinker Reactivities Amine-reac
- Page 21 and 22: Pyridyl disulfides Pyridyl disulfid
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- Page 33 and 34: Appendix I Structures Product # Pro
- Page 35 and 36: Appendix I Structures Product # Pro
- Page 37 and 38: Appendix I Structures Product # Pro
- Page 39: Appendix I Structures Product # Pro
- Page 43 and 44: Appendix I Structures Product # Pro
- Page 45 and 46: Appendix I Structures Product # Pro
- Page 47 and 48: Appendix III - Glossary of Crosslin
Appendix I Structures<br />
Product # Product Name Structure Reference<br />
22102<br />
22103<br />
SM(PEG) 2<br />
M.W. 425.39<br />
Spacer Arm 17.6 Å<br />
SM(PEG)2<br />
M.W. 425.39<br />
Spacer Arm 17.6 Å<br />
22104<br />
22107<br />
SM(PEG) 4<br />
M.W. 513.5<br />
Spacer Arm 24.6 Å<br />
SM(PEG)4<br />
M.W. 513.50<br />
Spacer Arm 24.6 Å<br />
[<br />
]<br />
22105 SM(PEG) 6<br />
M.W. 601.6<br />
Spacer Arm 32.5 Å<br />
SM(PEG)6<br />
M.W. 601.60<br />
Spacer Arm 32.5 Å<br />
[<br />
]<br />
22108 SM(PEG) 8<br />
M.W.689.71<br />
Spacer Arm 39.2 Å<br />
SM(PEG)8<br />
M.W. 689.71<br />
Spacer Arm 39.2 Å<br />
[<br />
]<br />
22112<br />
22113<br />
SM(PEG) 12<br />
M.W. 865.92<br />
Spacer Arm 53.4 Å<br />
SM(PEG)12<br />
M.W. 865.92<br />
Spacer Arm 53.4 Å<br />
[<br />
]<br />
22114 SM(PEG) 24<br />
M.W. 1394.55<br />
Spacer Arm 95.2 Å<br />
SM(PEG)24<br />
M.W. 1394.55<br />
Spacer Arm 95.2 Å<br />
[<br />
]<br />
22416 SMPB<br />
M.W. 356.33<br />
Spacer Arm 11.6 Å<br />
22363 SMPH<br />
M.W. 379.36<br />
Spacer Arm 14.2 Å<br />
SMPB<br />
M.W. 356.33<br />
Spacer Arm 11.6 Å<br />
• Thorpe, P.E., et al. (1984). Eur. J. Biochem 140, 63-71.<br />
• Rector, E.S., et al. (1978). J. Immunol. Methods 24, 321-336.<br />
SMPH<br />
M.W. 379.36<br />
Spacer Arm 14.3 Å<br />
To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.<br />
39
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