Thermo Scientific Pierce Crosslinking Technical Handbook

Appendix I Structures
Product # Product Name Structure Reference
77149
22980
22981
EDC
M.W. 191.70
Spacer Arm 0 Å
• Taniuchi, M., et al. (1986). Proc. Natl. Acad. Sci. USA 83,
4094-4098.
• Grabarek, Z. and Gergely, J. (1990). Anal. Biochem. 185(1),
131-135.
21565 EGS
M.W. 456.36
Spacer Arm 16.1 Å
• Millar, J.B. and Rozengurt, E. (1990). J. Biol. Chem. 265,
12052-12058.
• Browning, J. and Ribolini, A. (1989). J. Immunol. 143,
1859-1867. (EGS example)
22306 EMCA
• Griffith, D.G., et al. (1981). FEBS Lett. 134, 261-263.
M.W. 211.21
Spacer Arm 9.4 Å
22106 EMCH
• Trail, P.A., et al. (1993). Science 261, 212-215.
M.W. 225.24
Spacer Arm 11.8 Å
22308 EMCS
M.W. 308.29
Spacer Arm 9.4 Å
• Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83.
• Peeters, J.M., et al. (1989). J. Immunol. Methods 120,
133-143.
• Hermanson, G.T. (1996). Bioconjugate Techniques,
San Diego: Academic Press, 243-245. (EMCS use can be
modeled after GMBS.)
22309 GMBS
M.W. 280.23
Spacer Arm 7.3 Å
• Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83.
• Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15),
3031-3039.
22211 KMUA
M.W. 281.35
Spacer Arm 15.7 Å
• Rich, D.H., et al. (1975). J. Med. Chem. 18, 1004-1010.
• Moroder, L., et al. (1983). Biopolymers 22(1), 481-486.
22111 KMUH
• Trail, P.A., et al. (1993). Science 261, 212-215.
M.W. 295.38
Spacer Arm 19.0 Å
To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.
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