Thermo Scientific Pierce Crosslinking Technical Handbook
Thermo Scientific Pierce Crosslinking Technical Handbook Thermo Scientific Pierce Crosslinking Technical Handbook
Appendix I – Structures (continued) Appendix I Structures Product # Product Name Structure Reference 21610 BS 2 G-d 0 M.W. 530.35 Spacer Arm 7.7 Å O S O O O O O Na + O – O S O O – Na + N O O N O O 21615 BS 2 G-d 4 M.W. 534.38 Spacer Arm 7.7 Å O S O O O O O Na + O – O S O O – Na + N O O N O D D D D O 21580 BS 3 (Sulfo-DSS) M.W. 572.43 Spacer Arm 11.4 Å BS 2 G-d 4 M.W. 576.45 Spacer Arm 11.4 Å • Knoller, S., et al. (1991). J. Biol. Chem. 266, 2795-2804. 21590 BS 3 -d 0 M.W. 572.43 Spacer Arm 11.4 Å 21595 BS 3 -d 4 M.W. 576.45 Spacer Arm 11.4 Å 21581 BS(PEG) 5 M.W. 532.5 Spacer Arm 21.7 Å O O O O N O O [ ] 5 O O N O 21582 BS(PEG) 9 M.W. 708.71 Spacer Arm 35.8 Å O O Bis (NHS)PEO 5 M.W. 532.50 O O Spacer Arm 21.7 Å N O O [ ] 9 O O N O BS(PEG) 9 M.W. 708.71 Spacer Arm 35.8 Å 32 For more information, or to download product instructions, visit www.thermo.com/pierce
Appendix I Structures Product # Product Name Structure Reference 21600 BSOCOES M.W. 436.35 Spacer Arm 13.0 Å • Bouizar, Z., et al. (1986). Eur. J. Biochem. 155, 141-147. • Zarling, D.A., et al. (1980). J. Immunol. 124, 913-920. 22405 C6-SANH M.W. 403.43 Spacer Arm 14.4 Å 22423 C6-SFB M.W. 360.36 Spacer Arm 13.5 Å 20320 DCC M.W. 206.33 Spacer Arm 0 Å 21525 DFDNB M.W. 204.09 Spacer Arm 3.0 Å • Kornblatt, J.A. and Lake, D.F. (1980). Can J. Biochem. 58, 219-224. 20660 DMA M.W. 245.15 Spacer Arm 8.6 Å • Hartman, F.C. and Wold, F. (1967). Biochemistry 6(8), 2439-2448. 21666 21667 DMP M.W. 259.17 Spacer Arm 9.2 Å • Schneider, C., et al. (1982). J. Biol. Chem. 257(18), 10766-10769. 20700 DMS M.W. 273.20 Spacer Arm 11.0 Å • Wang, D. and Moore, S. (1977). Biochemistry 16(13), 2937-2942. To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor. 33
- Page 1 and 2: Thermo Scientific Pierce Crosslinki
- Page 3 and 4: Introduction What is crosslinking C
- Page 5 and 6: Crosslinking Applications Crosslink
- Page 7 and 8: Solid-phase immobilization Proteins
- Page 9 and 10: O NH Biotin HN S SO 3 - Cleavable D
- Page 11 and 12: Sulfo-SBED Reagent Thermo Scientifi
- Page 13 and 14: Figure 3. Applications of Sulfo-SBE
- Page 15 and 16: Structure determination with heavy/
- Page 17 and 18: Bioconjugate Toolkit The next gener
- Page 19 and 20: Crosslinker Reactivities Amine-reac
- Page 21 and 22: Pyridyl disulfides Pyridyl disulfid
- Page 23 and 24: Phenyl Azide UV Light Nitrene Forma
- Page 25 and 26: Books and Free Technical Handbooks!
- Page 27 and 28: - SH Sulfhydryls X Carbohydrates No
- Page 29 and 30: - SH Sulfhydryls X Carbohydrates No
- Page 31 and 32: - SH Sulfhydryls Carbohydrates Nons
- Page 33: Appendix I Structures Product # Pro
- Page 37 and 38: Appendix I Structures Product # Pro
- Page 39 and 40: Appendix I Structures Product # Pro
- Page 41 and 42: Appendix I Structures Product # Pro
- Page 43 and 44: Appendix I Structures Product # Pro
- Page 45 and 46: Appendix I Structures Product # Pro
- Page 47 and 48: Appendix III - Glossary of Crosslin
Appendix I – Structures (continued)<br />
Appendix I Structures<br />
Product # Product Name Structure Reference<br />
21610 BS 2 G-d 0<br />
M.W. 530.35<br />
Spacer Arm 7.7 Å<br />
O<br />
S<br />
O<br />
O<br />
O<br />
O<br />
O<br />
Na + O – O<br />
S<br />
O<br />
O – Na + N<br />
O<br />
O<br />
N<br />
O<br />
O<br />
21615 BS 2 G-d 4<br />
M.W. 534.38<br />
Spacer Arm 7.7 Å<br />
O<br />
S<br />
O<br />
O<br />
O<br />
O<br />
O<br />
Na + O – O<br />
S<br />
O<br />
O – Na + N<br />
O<br />
O<br />
N<br />
O<br />
D D D<br />
D<br />
O<br />
21580 BS 3 (Sulfo-DSS)<br />
M.W. 572.43<br />
Spacer Arm 11.4 Å<br />
BS 2 G-d 4<br />
M.W. 576.45<br />
Spacer Arm 11.4 Å<br />
• Knoller, S., et al. (1991). J. Biol. Chem. 266, 2795-2804.<br />
21590 BS 3 -d 0<br />
M.W. 572.43<br />
Spacer Arm 11.4 Å<br />
21595 BS 3 -d 4<br />
M.W. 576.45<br />
Spacer Arm 11.4 Å<br />
21581 BS(PEG) 5<br />
M.W. 532.5<br />
Spacer Arm 21.7 Å<br />
O<br />
O<br />
O O<br />
N O<br />
O [ ]<br />
5<br />
O<br />
O<br />
N<br />
O<br />
21582 BS(PEG) 9<br />
M.W. 708.71<br />
Spacer Arm 35.8 Å<br />
O<br />
O Bis (NHS)PEO 5<br />
M.W. 532.50<br />
O O<br />
Spacer Arm 21.7 Å<br />
N O<br />
O [ ]<br />
9<br />
O<br />
O<br />
N<br />
O<br />
BS(PEG) 9<br />
M.W. 708.71<br />
Spacer Arm 35.8 Å<br />
32<br />
For more information, or to download product instructions, visit www.thermo.com/pierce
Change language