Thermo Scientific Pierce Crosslinking Technical Handbook
Thermo Scientific Pierce Crosslinking Technical Handbook Thermo Scientific Pierce Crosslinking Technical Handbook
Appendix I – Structures Appendix I Structures Product # Product Name Structure Reference 21510 ABH M.W. 177.16 Spacer Arm 11.9 Å 22295 AMAS M.W. 252.18 Spacer Arm 4.4 Å • May, J.M. (1989). Biochemistry 28, 1718-1725. • Sayre, L.M., et al. (1984). J. Med. Chem. 27(10), 1325-35. 21451 ANB-NOS M.W. 305.20 Spacer Arm 7.7 Å • Krieg, U.C., et al. (1986). Proc. Natl. Acad. Sci. USA 83, 8604-8608. 27720 APDP M.W. 446.55 Spacer Arm 21.0 Å • Traut, R.R., et al. (1989). Protein Function, A Practical Approach. Oxford: IRL Press, p. 101. 21512 ASBA M.W. 249.27 Spacer Arm 16.3 Å • Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press, pp. 284, 416. 21564 BASED M.W. 474.52 Spacer Arm 21.3 Å • Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press, pp. 214, 416. 30 For more information, or to download product instructions, visit www.thermo.com/pierce
Appendix I Structures Product # Product Name Structure Reference 22331 BMB M.W. 248.23 Spacer Arm 10.9 Å • Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243. 22330 BMH • Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243. M.W. 276.29 Spacer Arm 16.1 Å 22323 BMOE • Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243. M.W. 220.18 Spacer Arm 8.0 Å 22297 BMPH M.W. 297.19 Spacer Arm 8.1 Å • Krieg, U.C., et al. (1986). Proc. Natl. Acad. Sci. USA 83, 8604-8608. • Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039. 22298 BMPS M.W. 266.21 Spacer Arm 5.9 Å • Kitagawa, T., et al. (1981). Chem. Pharm. Bull. 29(4), 1130-1135. • Kitagowa, T. (1981). Enzyme Immunoassay, Tokyo/New York, Igaku-Shoin pp. 81-89. 22336 BM(PEG) 2 M.W. 308.29 Spacer Arm 14.7 Å 22337 BM(PEG) 3 M.W. 352.34 Spacer Arm 17.8 Å To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor. 31
- Page 1 and 2: Thermo Scientific Pierce Crosslinki
- Page 3 and 4: Introduction What is crosslinking C
- Page 5 and 6: Crosslinking Applications Crosslink
- Page 7 and 8: Solid-phase immobilization Proteins
- Page 9 and 10: O NH Biotin HN S SO 3 - Cleavable D
- Page 11 and 12: Sulfo-SBED Reagent Thermo Scientifi
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- Page 15 and 16: Structure determination with heavy/
- Page 17 and 18: Bioconjugate Toolkit The next gener
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- Page 35 and 36: Appendix I Structures Product # Pro
- Page 37 and 38: Appendix I Structures Product # Pro
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Appendix I Structures<br />
Product # Product Name Structure Reference<br />
22331 BMB<br />
M.W. 248.23<br />
Spacer Arm 10.9 Å<br />
• Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243.<br />
22330 BMH<br />
• Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243.<br />
M.W. 276.29<br />
Spacer Arm 16.1 Å<br />
22323 BMOE<br />
• Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243.<br />
M.W. 220.18<br />
Spacer Arm 8.0 Å<br />
22297 BMPH<br />
M.W. 297.19<br />
Spacer Arm 8.1 Å<br />
• Krieg, U.C., et al. (1986). Proc. Natl. Acad. Sci. USA 83,<br />
8604-8608.<br />
• Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15),<br />
3031-3039.<br />
22298 BMPS<br />
M.W. 266.21<br />
Spacer Arm 5.9 Å<br />
• Kitagawa, T., et al. (1981). Chem. Pharm. Bull. 29(4),<br />
1130-1135.<br />
• Kitagowa, T. (1981). Enzyme Immunoassay,<br />
Tokyo/New York, Igaku-Shoin pp. 81-89.<br />
22336 BM(PEG) 2<br />
M.W. 308.29<br />
Spacer Arm 14.7 Å<br />
22337 BM(PEG) 3<br />
M.W. 352.34<br />
Spacer Arm 17.8 Å<br />
To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.<br />
31