Thermo Scientific Pierce Crosslinking Technical Handbook

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Thermo Scientific Pierce Crosslinkers at a Glance Product # Abbreviation Chemical Name Pkg. Size M.W. Spacer Arm – NH 2 Amines 22363 SMPH Succinimidyl-6-(β-maleimidopropionamido)hexanoate 50 mg 379.36 14.2 Å X 21558 SMPT 4-Succinimidyloxycarbonyl-methyl-α-(2-pyridyldithio)toluene 50 mg 388.46 20.0 Å X 23013 SPB Succinimidyl-(4-psoralen-8-yloxy)butyrate 50 mg 385.32 8.5-9.5 Å X 21857 SPDP N-Succinimidyl 3-(2-pyridyldithio)propionate 50 mg 312.37 6.8 Å X 21880 Sulfo-EGS See BS 3 21566 Sulfo-EGS Ethylene glycol bis (sulfo-succinimidyl succinate) 50 mg 660.45 16.1 Å X 22307 Sulfo-EMCS N-(ε-Maleimidocaproyloxy)sulfosuccinimide ester 50 mg 410.33 9.4 Å X 22324 Sulfo-GMBS N-(γ-Maleimidobutryloxy)sulfosuccinimide ester 50 mg 382.28 7.3 Å X 21563 Sulfo-HSAB N-Hydroxysulfosuccinimidyl-4-azidobenzoate 50 mg 362.25 9.0 Å X 21111 Sulfo-KMUS N-(κ-Maleimidoundecanoyloxy)sulfosuccinimide ester 50 mg 485.47 16.3 Å X 26174 Sulfo-LC-SDA Sulfo-NHS-LC-Diazirine 50 mg 440.40 12.5 X 21568 Sulfo-LC-SMPT Sulfosuccinimidyl 6-(α-methyl-α-[2-pyridyldithio]-toluamido) hexanoate 21650 Sulfo-LC-SPDP Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]propionamido)hexanoate 50 mg 603.67 20.0 Å X 50 mg 527.57 15.7 Å X 22312 Sulfo-MBS m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester 50 mg 416.30 7.3 Å X 27735 Sulfo-NHS-LC-ASA* Sulfosuccinimidyl(4-azido-salicylamido) hexanoate 50 mg 491.41 18.0 Å X 33030 Sulfo-SAED Sulfosuccimidyl 2-[7-azido-4-methylcoumarin- 3-acetamido]ethyl-1,3'-dithiopropionate 21549 Sulfo-SAND Sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido) ethyl 1,3'-dithiopropionate 22589 Sulfo-SANPAH Sulfosuccinimidyl 6-(4'-azido-2'- nitrophenylamino)hexanoate 5 mg 621.6 23.6 Å X 50 mg 570.51 18.5Å X 50 mg 492.40 18.2 Å X 33033 Sulfo-SBED** Sulfo-NHS-(2-6-[Biotinamido]-2-(p-azidobezamido) 10 mg 879.98 Sulfo-NHS ester 13.7 Å Phenyl azide 9.1 Å Biotin 19.1 Å 26173 Sulfo-SDA Sulfo-NHS-Diazirine 50 mg 327.25 3.9 X 26175 Sulfo-SDAD Sulfo-NHS-SS-Diazirine 50 mg 490.51 13.5 X 27719 Sulfo-SFAD Sulfosuccinimidyl(perfluoroazidobenzamido) ethyl 1,3'-dithiopropionate 50 mg 597.48 14.6 Å X 22327 Sulfo-SIAB Sulfosuccinimidyl(4-iodo-acetyl)aminobenzoate 50 mg 504.19 10.6 Å X 22322 Sulfo-SMCC Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate 50 mg 436.37 8.3 Å X 22317 Sulfo-SMPB Sulfosuccinimidyl 4-(p-maleimidophenyl)butyrate 50 mg 458.38 11.6 Å X 22607 THPP β-(Tris[hydroxymethyl]phosphine)propionic acid (betaine) 50 mg 197.15 3.03 Å X 33043 TMEA*** Tris-(2-Maleimidoethyl)amine (Trifunctional) 50 mg 386.36 10.3 Å 33063 TSAT*** Tris-(succimimidyl aminotricetate) (Trifunctional) 50 mg 482.36 4.2 Å X * Crosslinker is iodinatable. ** Trifunctional crosslinking reagent; binds to Avidin, Streptavidin or NeutrAvidin Protein. *** Trifunctional crosslinking reagent. **** Reacts selectively with arginine at pH 7-8. ***** Carbonyl reactive. ****** Hydrazine/Hydrazone reactive. 28 For more information, or to download product instructions, visit www.thermo.com/pierce
– SH Sulfhydryls Carbohydrates Nonselective (photo-reactive) – COOH Carboxyls –OH Hydroxyl Heterobifunctional Cleavable By X X Thiols X X X X Thiols X X Hydroxylamine X X X X X X X X X X X X Thiols X X Thiols X X X X X X Thiols X X Thiols X X X X X Thiols X X X X Thiols X X Thiols X X X X X X X To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor. 29
Page 1 and 2: Thermo Scientific Pierce Crosslinki
Page 3 and 4: Introduction What is crosslinking C
Page 5 and 6: Crosslinking Applications Crosslink
Page 7 and 8: Solid-phase immobilization Proteins
Page 9 and 10: O NH Biotin HN S SO 3 - Cleavable D
Page 11 and 12: Sulfo-SBED Reagent Thermo Scientifi
Page 13 and 14: Figure 3. Applications of Sulfo-SBE
Page 15 and 16: Structure determination with heavy/
Page 17 and 18: Bioconjugate Toolkit The next gener
Page 19 and 20: Crosslinker Reactivities Amine-reac
Page 21 and 22: Pyridyl disulfides Pyridyl disulfid
Page 23 and 24: Phenyl Azide UV Light Nitrene Forma
Page 25 and 26: Books and Free Technical Handbooks!
Page 27 and 28: - SH Sulfhydryls X Carbohydrates No
Page 29: - SH Sulfhydryls X Carbohydrates No
Page 33 and 34: Appendix I Structures Product # Pro
Page 47 and 48: Appendix III - Glossary of Crosslin

Thermo Scientific Pierce Crosslinking Technical Handbook

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