Thermo Scientific Pierce Crosslinking Technical Handbook

Thermo Scientific Pierce Crosslinkers at a Glance
Product # Abbreviation Chemical Name Pkg. Size M.W. Spacer Arm – NH 2 Amines
21565 EGS Ethylene glycol bis(succinimidylsuccinate) 1 g 456.36 16.1 Å X
22306 EMCA N-ε-Maleimidocaproic acid 1 g 211.21 9.4 Å X
22106 EMCH N-(ε-Maleimidocaproic acid)hydrazide 50 mg 225.24 11.8 Å
22308 EMCS N-(ε-Maleimidocaproyloxy)succinimide ester 50 mg 308.29 9.4 Å X
22309 GMBS N-(γ-Maleimidobutyryloxy)succinimide ester 50 mg 280.23 7.3 Å X
22211 KMUA N-κ-Maleimidoundecanoic acid 100 mg 281.35 15.7 Å X
22111 KMUH N-(κ-Maleimidoundecanoic acid)hydrazide 50 mg 295.38 19.0 Å
26168 LC-SDA NHS-LC-Diazirine 50 mg 338.36 12.5 X
22362 LC-SMCC Succinimidyl 4-(N-maleimidomethyl)
cyclohexane-1-carboxy-(6-amidocaproate)
21651 LC-SPDP Succinimidyl 6-(3'-[2-pyridyldithio]propionamido)hexanoate
50 mg 447.48 16.2 Å X
50 mg 425.52 15.7 Å X
22311 MBS m-Maleimidobenzoyl-N-hydroxysuccinimide ester 50 mg 314.25 7.3 Å X
22305 MPBH 4-(4-N-Maleimidophenyl)-butyric acid hydrazide•HCI 50 mg 309.75 17.9 Å
33093 Mts-Atf-Biotin** 2-[N2-(4-Azido-2,3,5,6-tetrafluorobenzoyl)-N6-
(6-biotinamidocaproyl)-L-lysinyl]ethylmethanethiosulfate
33083 Mts-Atf-LC-Biotin** 2-{N2-[N6-(4-Azido-2,3,5,6-tetrafluorobenzoyl)-N6-
(6-biotinamidocaproyl)-L-lysinyl]}ethylmethanethiosulfate
5 mg 839.95 Mts-Atf 11.1 Å
Mts-Biotin 29.3 ÅAtf-
Biotin 30.7 Å
5 mg 953.11 Mts-Atf 21.8 Å
Mts-Biotin 29.3 Å
Atf-Biotin 35.2 Å
27714 NHS-ASA* N-Hydroxysuccinimidyl-4-azidosalicylic acid 50 mg 276.21 8.0 Å X
22301 PDPH 3-(2-Pyridyldithio)propionylhydrazide 50 mg 229.32 9.2 Å
28100 PMPI N-(p-Maleimidophenyl)isocyanate 50 mg 214.18 8.7 Å
22400 SANH***** Succinimidyl 4-hydrazinonicotinate acetone hydrazone 25 mg 290.27 6.7 Å X
22600 SANPAH N-Succinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate 50 mg 390.35 18.2 Å X
22339 SBAP Succinimdyl 3-(bromoacetamido)propionate 50 mg 307.10 6.2 Å X
26167 SDA NHS-Diazirine 50 mg 225.20 3.9 X
26169 SDAD NHS-SS-Diazirine 50 mg 388.46 13.5 X
22419 SFB****** Succinimidyl 4-formylbenzoate 100 mg 247.20 5.8 Å X
22411 SHTH***** Succinimidyl 4-hydrazidoterephthalate hydrochloride 25 mg 311.68 7.9 Å X
22349 SIA N-succinimidyl iodoacetate 50 mg 283.02 1.5 Å X
22329 SIAB N-Succinimidyl(4-iodoacetyl)aminobenzoate 50 mg 402.14 10.6 Å X
22360 SMCC Succinimidyl 4-(N-maleimido-methyl)cyclohexane-1-
carboxylate
22102
22103
22104
22107
SM[PEG] 2
NHS-PEG 2 -Maliemide 100 mg
1 g
SM[PEG] 4
NHS-PEG 4 -Maliemide 100 mg
1 g
50 mg 334.32 8.3 Å X
425.39 17.6 Å X
513.5 24.6 Å X
22105 SM(PEG) 6 NHS-PEG 6 -Maleimide 100 mg 601.6 32.5 X
22108 SM[PEG] 8
NHS-PEG 8 -Maliemide 100 mg 689.71 39.2 Å X
22112
22113
SM[PEG] 12
NHS-PEG 12 -Maliemide 100 mg
1 g
865.92 53.4 Å X
22114 SM(PEG) 24 NHS-PEG 24 -Maleimide 102 mg 1394.55 95.2 X
22416 SMPB Succinimidyl 4-(p-maleimido-phenyl)butyrate 50 mg 356.33 11.6 Å X
* Crosslinker is iodinatable.
** Trifunctional crosslinking reagent; binds to Avidin, Streptavidin or NeutrAvidin Protein.
*** Trifunctional crosslinking reagent.
**** Reacts selectively with arginine at pH 7-8.
***** Carbonyl reactive.
****** Hydrazine/Hydrazone reactive.
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