Thermo Scientific Pierce Crosslinking Technical Handbook
Thermo Scientific Pierce Crosslinking Technical Handbook Thermo Scientific Pierce Crosslinking Technical Handbook
Thermo Scientific Pierce Crosslinkers at a Glance Product # Abbreviation Chemical Name Pkg. Size M.W. Spacer Arm – NH 2 Amines 21510 ABH p-Azidobenzoyl hydrazide 50 mg 177.16 11.9 Å 22295 AMAS N -(α-Maleimidoacetoxy)-succinimide ester 50 mg 252.18 4.4 Å X 21451 ANB-NOS N -5-Azido-2-nitrobenzyloxy-succinimide 50 mg 305.2 7.7 Å X 27720 APDP* N -(4-[p-Azidosalicylamido]butyl)- 3'-(2'-pyridyldithio) propionamide 50 mg 446.55 21.0 Å 21512 ASBA* 4-(p-Azidosalicylamido)-butylamine 50 mg 249.27 16.3 Å 21564 BASED* Bis (β-[4-azidosalicylamido]ethyl) disulfide 50 mg 474.52 21.3 Å 22331 BMB 1,4-Bis-Maleimidobutane 50 mg 248.23 10.9 Å 22330 BMH Bis-Maleimidohexane 50 mg 276.29 16.1 Å 22323 BMOE Bis-Maleimidoethane 50 mg 220.18 8.0 Å 22297 BMPH N -(β-Maleimidopropionic acid)hydrazide•TFA 50 mg 297.19 8.1 Å 22298 BMPS N -(β-Maleimidopropyloxy)succinimide ester 50 mg 266.21 5.9 Å X 22336 BM(PEG) 2 1,8-Bis-Maleimidodiethylene-glycol 50 mg 308.29 14.7 Å 22337 BM(PEG) 3 1,11-Bis-Maleimidotriethyleneglycol 50 mg 352.34 17.8 Å 21610 BS 2 G-d 0 Bis (sulfosuccinimidyl)glutarate-d 0 10 mg 530.35 7.7 Å X 21615 BS 2 G-d 4 Bis (sulfosuccinimidyl)2,2,4,4-glutarate-d 4 10 mg 534.38 7.7 Å X 21580 BS 3 (Sulfo-DSS) Bis (sulfosuccinimidyl)suberate 50 mg 572.43 11.4 Å X 21590 BS 3 -d 0 Bis (sulfosuccinimidyl)suberate-d 0 10 mg 572.43 11.4 Å X 21595 BS 3 -d 4 Bis (sulfosuccinimidyl)2,2,7,7-suberate-d 4 10 mg 576.45 11.4 Å X 21581 BS[PEG] 5 Bis (NHS)PEG 5 100 mg 532.5 21.7 Å X 21582 BS(PEG) 9 Bis (NHS)PEG 9 100 mg 708.71 35.8 X 21600 BSOCOES Bis(2-[succinimidoxycarbonyloxy]ethyl)sulfone 50 mg 436.35 13 Å X 22405 C6-SANH***** C6-Succinimidyl 4-hydrazinonicotinate acetone hydrazone 25 mg 403.43 14.4 Å X 22423 C6-SFB****** C6-Succinimidyl 4-formylbenzoate 25 mg 360.36 13.5 Å X 20320 DCC N,N-Dicyclohexylcarbodiimide 100 g 206.33 0 Å X 21525 DFDNB 1-5-Difluoro-2,4-dinitrobenzene 50 mg 204.09 3 Å X 20660 DMA Dimethyl adipimidate•2HCI 1 g 245.15 8.6 Å X 21666 21667 DMP Dimethyl pimelimidate•2HCI 50 mg 1 g 259.17 9.2 Å X 20700 DMS Dimethyl suberimidate•2HCl 1 g 273.20 11 Å X 21702 DPDPB 1,4-Di-(3'-[2'pyridyldithio]propionamido) butane 50 mg 482.71 19.9 Å 20593 DSG Disuccinimidyl glutarate 50 mg 326.26 7.7 Å X 22585 DSP Dithiobis(succimidylpropionate) (Lomant’s Reagent) 1 g 404.42 12 Å X 21655 21555 21658 DSS Disuccinimidyl suberate 50 mg 1 g 8 x 2 mg 368.35 11.4 Å X 20589 DST Disuccinimidyl tartarate 50 mg 344.24 6.4 Å X 20665 DTBP Dimethyl 3,3'-dithiobispropionimidate•2HC 1 g 309.28 11.9 Å X 22335 DTME Dithiobis-maleimidoethane 50 mg 312.37 13.3 Å 21578 DTSSP (Sulfo-DSP) 3,3'-Dithiobis (sulfosuccinimidylpropionate) 50 mg 608.51 12 Å X 77149 22980 22981 EDC 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride 10 mg 5 g 25 g 191.70 0 Å X * Crosslinker is iodinatable. ** Trifunctional crosslinking reagent; binds to Avidin, Streptavidin or NeutrAvidin Protein. *** Trifunctional crosslinking reagent. **** Reacts selectively with arginine at pH 7-8. ***** Carbonyl reactive. ****** Hydrazine/Hydrazone reactive. 24 For more information, or to download product instructions, visit www.thermo.com/pierce
– SH Sulfhydryls X Carbohydrates Nonselective (photo-reactive) – COOH Carboxyls –OH Hydroxyl Heterobifunctional X X X X X X Cleavable By X X X Thiols X X X X X X X X X X X X X X Thiols Base X X X Thiols Thiols X X X Periodate Thiols Thiols Thiols To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor. 25
- Page 1 and 2: Thermo Scientific Pierce Crosslinki
- Page 3 and 4: Introduction What is crosslinking C
- Page 5 and 6: Crosslinking Applications Crosslink
- Page 7 and 8: Solid-phase immobilization Proteins
- Page 9 and 10: O NH Biotin HN S SO 3 - Cleavable D
- Page 11 and 12: Sulfo-SBED Reagent Thermo Scientifi
- Page 13 and 14: Figure 3. Applications of Sulfo-SBE
- Page 15 and 16: Structure determination with heavy/
- Page 17 and 18: Bioconjugate Toolkit The next gener
- Page 19 and 20: Crosslinker Reactivities Amine-reac
- Page 21 and 22: Pyridyl disulfides Pyridyl disulfid
- Page 23 and 24: Phenyl Azide UV Light Nitrene Forma
- Page 25: Books and Free Technical Handbooks!
- Page 29 and 30: - SH Sulfhydryls X Carbohydrates No
- Page 31 and 32: - SH Sulfhydryls Carbohydrates Nons
- Page 33 and 34: Appendix I Structures Product # Pro
- Page 35 and 36: Appendix I Structures Product # Pro
- Page 37 and 38: Appendix I Structures Product # Pro
- Page 39 and 40: Appendix I Structures Product # Pro
- Page 41 and 42: Appendix I Structures Product # Pro
- Page 43 and 44: Appendix I Structures Product # Pro
- Page 45 and 46: Appendix I Structures Product # Pro
- Page 47 and 48: Appendix III - Glossary of Crosslin
<strong>Thermo</strong> <strong>Scientific</strong> <strong>Pierce</strong> Crosslinkers at a Glance<br />
Product # Abbreviation Chemical Name Pkg. Size M.W. Spacer Arm – NH 2 Amines<br />
21510 ABH p-Azidobenzoyl hydrazide 50 mg 177.16 11.9 Å<br />
22295 AMAS N -(α-Maleimidoacetoxy)-succinimide ester 50 mg 252.18 4.4 Å X<br />
21451 ANB-NOS N -5-Azido-2-nitrobenzyloxy-succinimide 50 mg 305.2 7.7 Å X<br />
27720 APDP* N -(4-[p-Azidosalicylamido]butyl)-<br />
3'-(2'-pyridyldithio) propionamide<br />
50 mg 446.55 21.0 Å<br />
21512 ASBA* 4-(p-Azidosalicylamido)-butylamine 50 mg 249.27 16.3 Å<br />
21564 BASED* Bis (β-[4-azidosalicylamido]ethyl) disulfide 50 mg 474.52 21.3 Å<br />
22331 BMB 1,4-Bis-Maleimidobutane 50 mg 248.23 10.9 Å<br />
22330 BMH Bis-Maleimidohexane 50 mg 276.29 16.1 Å<br />
22323 BMOE Bis-Maleimidoethane 50 mg 220.18 8.0 Å<br />
22297 BMPH N -(β-Maleimidopropionic acid)hydrazide•TFA 50 mg 297.19 8.1 Å<br />
22298 BMPS N -(β-Maleimidopropyloxy)succinimide ester 50 mg 266.21 5.9 Å X<br />
22336 BM(PEG) 2 1,8-Bis-Maleimidodiethylene-glycol 50 mg 308.29 14.7 Å<br />
22337 BM(PEG) 3 1,11-Bis-Maleimidotriethyleneglycol 50 mg 352.34 17.8 Å<br />
21610 BS 2 G-d 0 Bis (sulfosuccinimidyl)glutarate-d 0 10 mg 530.35 7.7 Å X<br />
21615 BS 2 G-d 4 Bis (sulfosuccinimidyl)2,2,4,4-glutarate-d 4 10 mg 534.38 7.7 Å X<br />
21580 BS 3 (Sulfo-DSS) Bis (sulfosuccinimidyl)suberate 50 mg 572.43 11.4 Å X<br />
21590 BS 3 -d 0 Bis (sulfosuccinimidyl)suberate-d 0 10 mg 572.43 11.4 Å X<br />
21595 BS 3 -d 4 Bis (sulfosuccinimidyl)2,2,7,7-suberate-d 4 10 mg 576.45 11.4 Å X<br />
21581 BS[PEG] 5<br />
Bis (NHS)PEG 5 100 mg 532.5 21.7 Å X<br />
21582 BS(PEG) 9 Bis (NHS)PEG 9 100 mg 708.71 35.8 X<br />
21600 BSOCOES Bis(2-[succinimidoxycarbonyloxy]ethyl)sulfone 50 mg 436.35 13 Å X<br />
22405 C6-SANH***** C6-Succinimidyl 4-hydrazinonicotinate acetone hydrazone 25 mg 403.43 14.4 Å X<br />
22423 C6-SFB****** C6-Succinimidyl 4-formylbenzoate 25 mg 360.36 13.5 Å X<br />
20320 DCC N,N-Dicyclohexylcarbodiimide 100 g 206.33 0 Å X<br />
21525 DFDNB 1-5-Difluoro-2,4-dinitrobenzene 50 mg 204.09 3 Å X<br />
20660 DMA Dimethyl adipimidate•2HCI 1 g 245.15 8.6 Å X<br />
21666<br />
21667<br />
DMP Dimethyl pimelimidate•2HCI 50 mg<br />
1 g<br />
259.17 9.2 Å X<br />
20700 DMS Dimethyl suberimidate•2HCl 1 g 273.20 11 Å X<br />
21702 DPDPB 1,4-Di-(3'-[2'pyridyldithio]propionamido) butane 50 mg 482.71 19.9 Å<br />
20593 DSG Disuccinimidyl glutarate 50 mg 326.26 7.7 Å X<br />
22585 DSP Dithiobis(succimidylpropionate) (Lomant’s Reagent) 1 g 404.42 12 Å X<br />
21655<br />
21555<br />
21658<br />
DSS Disuccinimidyl suberate 50 mg<br />
1 g<br />
8 x 2 mg<br />
368.35 11.4 Å X<br />
20589 DST Disuccinimidyl tartarate 50 mg 344.24 6.4 Å X<br />
20665 DTBP Dimethyl 3,3'-dithiobispropionimidate•2HC 1 g 309.28 11.9 Å X<br />
22335 DTME Dithiobis-maleimidoethane 50 mg 312.37 13.3 Å<br />
21578 DTSSP (Sulfo-DSP) 3,3'-Dithiobis (sulfosuccinimidylpropionate) 50 mg 608.51 12 Å X<br />
77149<br />
22980<br />
22981<br />
EDC<br />
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide<br />
hydrochloride<br />
10 mg<br />
5 g<br />
25 g<br />
191.70 0 Å X<br />
* Crosslinker is iodinatable.<br />
** Trifunctional crosslinking reagent; binds to Avidin, Streptavidin or NeutrAvidin Protein.<br />
*** Trifunctional crosslinking reagent.<br />
**** Reacts selectively with arginine at pH 7-8.<br />
***** Carbonyl reactive.<br />
****** Hydrazine/Hydrazone reactive.<br />
24<br />
For more information, or to download product instructions, visit www.thermo.com/pierce
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